Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cell Calcium
2009 Jan 01;451:65-76. doi: 10.1016/j.ceca.2008.06.001.
Show Gene links
Show Anatomy links
Localization of puff sites adjacent to the plasma membrane: functional and spatial characterization of Ca2+ signaling in SH-SY5Y cells utilizing membrane-permeant caged IP3.
Smith IF
,
Wiltgen SM
,
Parker I
.
???displayArticle.abstract???
The Xenopus oocyte has been a favored model system in which to study spatio-temporal mechanisms of intracellular Ca2+ dynamics, in large part because this giant cell facilitates intracellular injections of Ca2+ indicator dyes, buffers and caged compounds. However, the recent commercial availability of membrane-permeant ester forms of caged IP3 (ci-IP3) and EGTA, now allows for facile loading of these compounds into smaller mammalian cells, permitting control of [IP3]i and cytosolic Ca2+ buffering. Here, we establish the human neuroblastoma SH-SY5Y cell line as an advantageous experimental system for imaging Ca2+ signaling, and characterize IP3-mediated Ca2+ signaling mechanisms in these cells. Flash photo-release of increasing amounts of i-IP3 evokes Ca2+ puffs that transition to waves, but intracellular loading of EGTA decouples release sites, allowing discrete puffs to be studied over a wide range of [IP3]. Puff activity persists for minutes following a single photo-release, pointing to a slow rate of i-IP3 turnover in these cells and suggesting that repetitive Ca2+ spikes with periods of 20-30s are not driven by oscillations in [IP3]. Puff amplitudes are independent of [IP3], whereas their frequencies increase with increasing photo-release. Puff sites in SH-SY5Y cells are not preferentially localized near the nucleus, but instead are concentrated close to the plasma membrane where they can be visualized by total internal reflection microscopy, offering the potential for unprecedented spatio-temporal resolution of Ca2+ puff kinetics.
Allbritton,
Range of messenger action of calcium ion and inositol 1,4,5-trisphosphate.
1992, Pubmed,
Xenbase
Allbritton,
Range of messenger action of calcium ion and inositol 1,4,5-trisphosphate.
1992,
Pubmed
,
Xenbase
Berridge,
Elementary and global aspects of calcium signalling.
1997,
Pubmed
Bezprozvanny,
Bell-shaped calcium-response curves of Ins(1,4,5)P3- and calcium-gated channels from endoplasmic reticulum of cerebellum.
1991,
Pubmed
Bootman,
Imaging the hierarchical Ca2+ signalling system in HeLa cells.
1997,
Pubmed
Bootman,
Cooking with calcium: the recipes for composing global signals from elementary events.
1997,
Pubmed
Busa,
Activation of frog (Xenopus laevis) eggs by inositol trisphosphate. I. Characterization of Ca2+ release from intracellular stores.
1985,
Pubmed
,
Xenbase
Callamaras,
Caged inositol 1,4,5-trisphosphate for studying release of Ca2+ from intracellular stores.
1998,
Pubmed
,
Xenbase
Callamaras,
Radial localization of inositol 1,4,5-trisphosphate-sensitive Ca2+ release sites in Xenopus oocytes resolved by axial confocal linescan imaging.
1999,
Pubmed
,
Xenbase
Callamaras,
Phasic characteristic of elementary Ca(2+) release sites underlies quantal responses to IP(3).
2000,
Pubmed
,
Xenbase
Callamaras,
Activation and co-ordination of InsP3-mediated elementary Ca2+ events during global Ca2+ signals in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Dakin,
Cell membrane permeable esters of D-myo-inositol 1,4,5-trisphosphate.
2007,
Pubmed
Dargan,
Imaging Ca2+ signals in Xenopus oocytes.
2006,
Pubmed
,
Xenbase
Dargan,
Buffer kinetics shape the spatiotemporal patterns of IP3-evoked Ca2+ signals.
2003,
Pubmed
,
Xenbase
Dargan,
Spatiotemporal patterning of IP3-mediated Ca2+ signals in Xenopus oocytes by Ca2+-binding proteins.
2004,
Pubmed
,
Xenbase
DeLisle,
Inositol trisphosphate is required for the propagation of calcium waves in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Demuro,
"Optical patch-clamping": single-channel recording by imaging Ca2+ flux through individual muscle acetylcholine receptor channels.
2005,
Pubmed
,
Xenbase
Demuro,
Multi-dimensional resolution of elementary Ca2+ signals by simultaneous multi-focal imaging.
2008,
Pubmed
,
Xenbase
Fogarty,
Mechanisms underlying InsP3-evoked global Ca2+ signals in mouse pancreatic acinar cells.
2000,
Pubmed
Foskett,
Inositol trisphosphate receptor Ca2+ release channels.
2007,
Pubmed
Hillson,
Localised and rapid Ca2+ micro-events in human neutrophils: conventional Ca2+ puffs and global waves without peripheral-restriction or wave cycling.
2007,
Pubmed
Hirose,
Spatiotemporal dynamics of inositol 1,4,5-trisphosphate that underlies complex Ca2+ mobilization patterns.
1999,
Pubmed
Hoesch,
Localized IP3-evoked Ca2+ release activates a K+ current in primary vagal sensory neurons.
2004,
Pubmed
Izu,
Theoretical analysis of the Ca2+ spark amplitude distribution.
1998,
Pubmed
Koizumi,
Characterization of elementary Ca2+ release signals in NGF-differentiated PC12 cells and hippocampal neurons.
1999,
Pubmed
Lechleiter,
Molecular mechanisms of intracellular calcium excitability in X. laevis oocytes.
1992,
Pubmed
,
Xenbase
Li,
Cell-permeant caged InsP3 ester shows that Ca2+ spike frequency can optimize gene expression.
1998,
Pubmed
Lipp,
A hierarchical concept of cellular and subcellular Ca(2+)-signalling.
1996,
Pubmed
Lipp,
Nuclear calcium signalling by individual cytoplasmic calcium puffs.
1997,
Pubmed
Marchant,
Functional interactions in Ca(2+) signaling over different time and distance scales.
2000,
Pubmed
,
Xenbase
Marchant,
Role of elementary Ca(2+) puffs in generating repetitive Ca(2+) oscillations.
2001,
Pubmed
,
Xenbase
Matsu-ura,
Cytosolic inositol 1,4,5-trisphosphate dynamics during intracellular calcium oscillations in living cells.
2006,
Pubmed
Parker,
Photonics for biologists.
2003,
Pubmed
Parker,
Changes in intracellular calcium and in membrane currents evoked by injection of inositol trisphosphate into Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Parker,
Regenerative release of calcium from functionally discrete subcellular stores by inositol trisphosphate.
1991,
Pubmed
,
Xenbase
Parker,
Confocal microfluorimetry of Ca2+ signals evoked in Xenopus oocytes by photoreleased inositol trisphosphate.
1993,
Pubmed
,
Xenbase
Parker,
Ca2+ transients associated with openings of inositol trisphosphate-gated channels in Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Parker,
Elementary events of InsP3-induced Ca2+ liberation in Xenopus oocytes: hot spots, puffs and blips.
1996,
Pubmed
,
Xenbase
Parys,
Isolation, characterization, and localization of the inositol 1,4,5-trisphosphate receptor protein in Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
Riddoch,
Release and sequestration of Ca2+ by a caffeine- and ryanodine-sensitive store in a sub-population of human SH-SY5Y neuroblastoma cells.
2005,
Pubmed
Rose,
'Trigger' events precede calcium puffs in Xenopus oocytes.
2006,
Pubmed
,
Xenbase
Samways,
Co-incident signalling between mu-opioid and M3 muscarinic receptors at the level of Ca2+ release from intracellular stores: lack of evidence for Ins(1,4,5)P3 receptor sensitization.
2003,
Pubmed
Shuai,
Optical single-channel recording by imaging Ca2+ flux through individual ion channels: theoretical considerations and limits to resolution.
2005,
Pubmed
,
Xenbase
Shuai,
A kinetic model of single and clustered IP3 receptors in the absence of Ca2+ feedback.
2007,
Pubmed
,
Xenbase
Stutzmann,
Enhanced ryanodine receptor recruitment contributes to Ca2+ disruptions in young, adult, and aged Alzheimer's disease mice.
2006,
Pubmed
Stutzmann,
Ca2+ signaling in mouse cortical neurons studied by two-photon imaging and photoreleased inositol triphosphate.
2003,
Pubmed
Stutzmann,
Dysregulated IP3 signaling in cortical neurons of knock-in mice expressing an Alzheimer's-linked mutation in presenilin1 results in exaggerated Ca2+ signals and altered membrane excitability.
2004,
Pubmed
Thomas,
Hormone-evoked elementary Ca2+ signals are not stereotypic, but reflect activation of different size channel clusters and variable recruitment of channels within a cluster.
1998,
Pubmed
Thomas,
Microscopic properties of elementary Ca2+ release sites in non-excitable cells.
2000,
Pubmed
Thorn,
Local and global cytosolic Ca2+ oscillations in exocrine cells evoked by agonists and inositol trisphosphate.
1993,
Pubmed
Tovey,
Calcium puffs are generic InsP(3)-activated elementary calcium signals and are downregulated by prolonged hormonal stimulation to inhibit cellular calcium responses.
2001,
Pubmed
Van Acker,
IP(3)-mediated Ca(2+) signals in human neuroblastoma SH-SY5Y cells with exogenous overexpression of type 3 IP(3) receptor.
2002,
Pubmed
Wagner,
Phosphorylation of type-1 inositol 1,4,5-trisphosphate receptors by cyclic nucleotide-dependent protein kinases: a mutational analysis of the functionally important sites in the S2+ and S2- splice variants.
2003,
Pubmed
Yao,
Ca2+ influx modulation of temporal and spatial patterns of inositol trisphosphate-mediated Ca2+ liberation in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Yao,
Quantal puffs of intracellular Ca2+ evoked by inositol trisphosphate in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
