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XB-ART-38157
Nat Genet. July 1, 2008; 40 (7): 871-9.

Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells.

Park TJ , Mitchell BJ , Abitua PB , Kintner C , Wallingford JB .


Abstract
The planar cell polarity (PCP) signaling system governs many aspects of polarized cell behavior. Here, we use an in vivo model of vertebrate mucociliary epithelial development to show that Dishevelled (Dvl) is essential for the apical positioning of basal bodies. We find that Dvl and Inturned mediate the activation of the Rho GTPase specifically at basal bodies, and that these three proteins together mediate the docking of basal bodies to the apical plasma membrane. Moreover, we find that this docking involves a Dvl-dependent association of basal bodies with membrane-bound vesicles and the vesicle-trafficking protein, Sec8. Once docked, basal bodies again require Dvl and Rho for the planar polarization that underlies directional beating of cilia. These results demonstrate previously undescribed functions for PCP signaling components and suggest that a common signaling apparatus governs both apical docking and planar polarization of basal bodies.

PubMed ID: 18552847
PMC ID: PMC2771675
Article link: Nat Genet.
Grant support: R01 GM074104-04 NIGMS NIH HHS , R01 GM074104-04S1 NIGMS NIH HHS , R01 GM076507-04 NIGMS NIH HHS , R01 GM074104 NIGMS NIH HHS , R01 GM076507 NIGMS NIH HHS , R01 GM074104-04S1 NIGMS NIH HHS , R01 GM074104 NIGMS NIH HHS , R01 GM076507 NIGMS NIH HHS , R01 GM074104-04 NIGMS NIH HHS , R01 GM076507-04 NIGMS NIH HHS

Genes referenced: cetn1 dvl1 dvl2 exoc4 intu rho rho.2 tjp1 tuba4b tubg1 tuba8

Antibodies referenced: Cetn1 Ab1 Dvl2 Ab2 Dvl3 Ab1 Tjp1 Ab1 Tuba4b Ab2 Tubg1 Ab3
Morpholinos referenced: dvl1 MO1 dvl2 MO1 dvl3 MO1 p2rx1 MO1

References:
Adler, 2004, Pubmed[+]


Article Images: [+] show captions

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