Due to necessary maintenance, Xenbase will be unavailable December 24-30, 2014. We apologize for the inconvenience.

Click on this message to dismiss it.
Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Nature. June 19, 2008; 453 (7198): 1132-6.

Midzone activation of aurora B in anaphase produces an intracellular phosphorylation gradient.

Fuller BG , Lampson MA , Foley EA , Rosasco-Nitcher S , Le KV , Tobelmann P , Brautigan DL , Stukenberg PT , Kapoor TM .

Proper partitioning of the contents of a cell between two daughters requires integration of spatial and temporal cues. The anaphase array of microtubules that self-organize at the spindle midzone contributes to positioning the cell-division plane midway between the segregating chromosomes. How this signalling occurs over length scales of micrometres, from the midzone to the cell cortex, is not known. Here we examine the anaphase dynamics of protein phosphorylation by aurora B kinase, a key mitotic regulator, using fluorescence resonance energy transfer (FRET)-based sensors in living HeLa cells and immunofluorescence of native aurora B substrates. Quantitative analysis of phosphorylation dynamics, using chromosome- and centromere-targeted sensors, reveals that changes are due primarily to position along the division axis rather than time. These dynamics result in the formation of a spatial phosphorylation gradient early in anaphase that is centred at the spindle midzone. This gradient depends on aurora B targeting to a subpopulation of microtubules that activate it. Aurora kinase activity organizes the targeted microtubules to generate a structure-based feedback loop. We propose that feedback between aurora B kinase activation and midzone microtubules generates a gradient of post-translational marks that provides spatial information for events in anaphase and cytokinesis.

PubMed ID: 18463638
PMC ID: PMC2724008
Article link: Nature.
Grant support: R01 GM063045-08 NIGMS NIH HHS , R01 GM065933-06 NIGMS NIH HHS , R01 GM083988-01 NIGMS NIH HHS , R01 GM083988-05 NIGMS NIH HHS , R01 GM063045 NIGMS NIH HHS , R01 GM065933 NIGMS NIH HHS , R01 GM083988 NIGMS NIH HHS

Genes referenced: aurkb
Antibodies referenced:
Morpholinos referenced:

My Xenbase: [ Log-in / Register ]
version: [3.3.1]

Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556