Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
We identified a functional domain (XlePABP2-TRP) of Xenopus laevis embryonic type II poly(A)-binding protein (XlePABP2). The NMR structure of XlePABP2-TRP revealed that the protein is a homodimer formed by the antiparallel association of beta-strands from the single RNA recognition motif (RRM) domain of each subunit. In each subunit of the homodimer, the canonical RNA recognition site is occluded by a polyproline motif. Upon poly(A) binding, XlePABP2-TRP undergoes a dimer-monomer transition that removes the polyproline motif from the RNA recognition site and allows it to be replaced by the adenosine nucleotides of poly(A). Our results provide high-resolution structural information concerning type II PABPs and an example of a single RRM domain protein that transitions from a homodimer to a monomer upon RNA binding. These findings advance our understanding of RRM domain regulation, poly(A) recognition, and are relevant to understanding how type II PABPs function in mRNA processing and human disease.
Allain,
Specificity of ribonucleoprotein interaction determined by RNA folding during complex formulation.
1996, Pubmed
Allain,
Specificity of ribonucleoprotein interaction determined by RNA folding during complex formulation.
1996,
Pubmed
Bienroth,
Assembly of a processive messenger RNA polyadenylation complex.
1993,
Pubmed
Brünger,
Crystallography & NMR system: A new software suite for macromolecular structure determination.
1998,
Pubmed
Chartier,
A Drosophila model of oculopharyngeal muscular dystrophy reveals intrinsic toxicity of PABPN1.
2006,
Pubmed
Cléry,
RNA recognition motifs: boring? Not quite.
2008,
Pubmed
Cornilescu,
Protein backbone angle restraints from searching a database for chemical shift and sequence homology.
1999,
Pubmed
Cosson,
Identification of a novel Xenopus laevis poly (A) binding protein.
2004,
Pubmed
,
Xenbase
Deo,
Recognition of polyadenylate RNA by the poly(A)-binding protein.
1999,
Pubmed
Good,
Xenopus embryonic poly(A) binding protein 2 (ePABP2) defines a new family of cytoplasmic poly(A) binding proteins expressed during the early stages of vertebrate development.
2004,
Pubmed
,
Xenbase
Güntert,
Torsion angle dynamics for NMR structure calculation with the new program DYANA.
1997,
Pubmed
Handa,
Structural basis for recognition of the tra mRNA precursor by the Sex-lethal protein.
1999,
Pubmed
Jeon,
High-throughput purification and quality assurance of Arabidopsis thaliana proteins for eukaryotic structural genomics.
2005,
Pubmed
Kahvejian,
The mRNA closed-loop model: the function of PABP and PABP-interacting proteins in mRNA translation.
2001,
Pubmed
Kühn,
The RNA binding domains of the nuclear poly(A)-binding protein.
2003,
Pubmed
Kühn,
Structure and function of poly(A) binding proteins.
2004,
Pubmed
Laskowski,
AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR.
1996,
Pubmed
Linge,
Refinement of protein structures in explicit solvent.
2003,
Pubmed
Mangus,
Poly(A)-binding proteins: multifunctional scaffolds for the post-transcriptional control of gene expression.
2003,
Pubmed
Maris,
The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression.
2005,
Pubmed
Mazza,
Large-scale induced fit recognition of an m(7)GpppG cap analogue by the human nuclear cap-binding complex.
2002,
Pubmed
,
Xenbase
Messaed,
Soluble expanded PABPN1 promotes cell death in oculopharyngeal muscular dystrophy.
2007,
Pubmed
Nagai,
The RNP domain: a sequence-specific RNA-binding domain involved in processing and transport of RNA.
1995,
Pubmed
Schwieters,
The Xplor-NIH NMR molecular structure determination package.
2003,
Pubmed
Song,
Three-dimensional structure determined for a subunit of human tRNA splicing endonuclease (Sen15) reveals a novel dimeric fold.
2007,
Pubmed
Sorokina,
Archived gels as a tool for identification of protein complexes: Polo kinase cofractionates with Drosophila 205-kDa MAP and ncd in mitotic embryonic extracts.
2005,
Pubmed
Thao,
Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination.
2004,
Pubmed
Tyler,
Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination.
2005,
Pubmed
Varani,
RNA recognition by RNP proteins during RNA processing.
1998,
Pubmed
Wahle,
Purification and characterization of a mammalian polyadenylate polymerase involved in the 3' end processing of messenger RNA precursors.
1991,
Pubmed
Wahle,
Mammalian poly(A)-binding protein II. Physical properties and binding to polynucleotides.
1993,
Pubmed
Wang,
Structural basis for recognition of AU-rich element RNA by the HuD protein.
2001,
Pubmed
Wells,
Circularization of mRNA by eukaryotic translation initiation factors.
1998,
Pubmed
Zweckstetter,
Prediction of charge-induced molecular alignment of biomolecules dissolved in dilute liquid-crystalline phases.
2004,
Pubmed
