XB-ART-38587
J Biol Chem
January 23, 2009;
284
(4):
2397-408.
Eomesodermin requires transforming growth factor-beta/activin signaling and binds Smad2 to activate mesodermal genes.
Picozzi P
,
Wang F
,
Cronk K
,
Ryan K
.
Abstract
The T-box gene
Eomesodermin (
Eomes) is required for early embryonic
mesoderm differentiation in mouse, frog (Xenopus laevis), and zebrafish, is important in late cardiac development in Xenopus, and for CD8+ T effector cell function in mouse.
Eomes can ectopically activate many mesodermal genes. However, the mechanism by which
Eomes activates transcription of these genes is poorly understood. We report that
Eomes protein interacts with
Smad2 and is capable of working in a non-cell autonomous manner via transfer of
Eomes protein between adjacent embryonic cells. Blocking of
Eomes protein transfer using a farnesylated red fluorescent protein (CherryF) also prevents
Eomes nuclear accumulation. Transfer of
Eomes protein between cells is mediated by the
Eomes carboxyl terminus (456-692). A carbohydrate binding domain within the
Eomes carboxyl-terminal region is sufficient for transfer and important for gene activation. We propose a novel mechanism by which
Eomes helps effect a cellular response to a morphogen gradient.
PubMed ID:
19036723
PMC ID:
PMC2629102
Article link:
J Biol Chem
Grant support:
[+]
Species referenced:
Xenopus laevis
Genes referenced:
eomes
smad2
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