XB-ART-38587
J Biol Chem
January 23, 2009;
284
(4):
2397-408.
Eomesodermin requires transforming growth factor-beta/activin signaling and binds Smad2 to activate mesodermal genes.
Picozzi P
,
Wang F
,
Cronk K
,
Ryan K
.
Abstract
The T-box gene
Eomesodermin (
Eomes) is required for early embryonic
mesoderm differentiation in mouse, frog (Xenopus laevis), and zebrafish, is important in late cardiac development in Xenopus, and for CD8+ T effector cell function in mouse.
Eomes can ectopically activate many mesodermal genes. However, the mechanism by which
Eomes activates transcription of these genes is poorly understood. We report that
Eomes protein interacts with
Smad2 and is capable of working in a non-cell autonomous manner via transfer of
Eomes protein between adjacent embryonic cells. Blocking of
Eomes protein transfer using a farnesylated red fluorescent protein (CherryF) also prevents
Eomes nuclear accumulation. Transfer of
Eomes protein between cells is mediated by the
Eomes carboxyl terminus (456-692). A carbohydrate binding domain within the
Eomes carboxyl-terminal region is sufficient for transfer and important for gene activation. We propose a novel mechanism by which
Eomes helps effect a cellular response to a morphogen gradient.
PubMed ID:
19036723
PMC ID:
PMC2629102
Article link:
J Biol Chem
Grant support:
[+]
Species referenced:
Xenopus laevis
Genes referenced:
eomes
smad2
References [+] :
Altschul,
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
1997,
Pubmed
Altschul,
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
1997,
Pubmed
Altschul,
Protein database searches using compositionally adjusted substitution matrices.
2005,
Pubmed
Apolloni,
H-ras but not K-ras traffics to the plasma membrane through the exocytic pathway.
2000,
Pubmed
Arnold,
Pivotal roles for eomesodermin during axis formation, epithelium-to-mesenchyme transition and endoderm specification in the mouse.
2008,
Pubmed
Ataliotis,
XTbx1 is a transcriptional activator involved in head and pharyngeal arch development in Xenopus laevis.
2005,
Pubmed
,
Xenbase
Bjornson,
Eomesodermin is a localized maternal determinant required for endoderm induction in zebrafish.
2005,
Pubmed
Bruce,
The maternally expressed zebrafish T-box gene eomesodermin regulates organizer formation.
2003,
Pubmed
,
Xenbase
Bruce,
T-box gene eomesodermin and the homeobox-containing Mix/Bix gene mtx2 regulate epiboly movements in the zebrafish.
2005,
Pubmed
Bulfone,
Expression pattern of the Tbr2 (Eomesodermin) gene during mouse and chick brain development.
1999,
Pubmed
,
Xenbase
Cardarelli,
In vivo study of HIV-1 Tat arginine-rich motif unveils its transport properties.
2007,
Pubmed
Ciruna,
Expression of the T-box gene Eomesodermin during early mouse development.
1999,
Pubmed
,
Xenbase
Conlon,
Determinants of T box protein specificity.
2001,
Pubmed
,
Xenbase
Derynck,
Nomenclature: vertebrate mediators of TGFbeta family signals.
1996,
Pubmed
,
Xenbase
Glimcher,
Recent developments in the transcriptional regulation of cytolytic effector cells.
2004,
Pubmed
Graff,
Xenopus Mad proteins transduce distinct subsets of signals for the TGF beta superfamily.
1996,
Pubmed
,
Xenbase
Hancock,
Mapping and expression analysis of the mouse ortholog of Xenopus Eomesodermin.
1999,
Pubmed
,
Xenbase
Intlekofer,
Effector and memory CD8+ T cell fate coupled by T-bet and eomesodermin.
2005,
Pubmed
Joliot,
Transduction peptides: from technology to physiology.
2004,
Pubmed
Malo,
X-ray structure of Cerulean GFP: a tryptophan-based chromophore useful for fluorescence lifetime imaging.
2007,
Pubmed
Massagué,
The logic of TGFbeta signaling.
2006,
Pubmed
Massagué,
Smad transcription factors.
2005,
Pubmed
Messenger,
Functional specificity of the Xenopus T-domain protein Brachyury is conferred by its ability to interact with Smad1.
2005,
Pubmed
,
Xenbase
Newfeld,
Mothers against dpp encodes a conserved cytoplasmic protein required in DPP/TGF-beta responsive cells.
1996,
Pubmed
,
Xenbase
Pearce,
Control of effector CD8+ T cell function by the transcription factor Eomesodermin.
2003,
Pubmed
Russ,
Eomesodermin is required for mouse trophoblast development and mesoderm formation.
2000,
Pubmed
,
Xenbase
Ryan,
The Xenopus eomesodermin promoter and its concentration-dependent response to activin.
2000,
Pubmed
,
Xenbase
Ryan,
T-box genes and cardiac development.
2003,
Pubmed
Ryan,
Modulation of eomes activity alters the size of the developing heart: implications for in utero cardiac gene therapy.
2004,
Pubmed
,
Xenbase
Ryan,
Eomesodermin, a key early gene in Xenopus mesoderm differentiation.
1997,
Pubmed
,
Xenbase
Ryan,
Xenopus eomesodermin is expressed in neural differentiation.
1999,
Pubmed
,
Xenbase
Showell,
T-box genes in early embryogenesis.
2003,
Pubmed
,
Xenbase
Shu,
Novel chromophores and buried charges control color in mFruits.
2006,
Pubmed
Smith,
T-box genes: what they do and how they do it.
1999,
Pubmed
Spemann,
Induction of embryonic primordia by implantation of organizers from a different species. 1923.
2001,
Pubmed
Standley,
A dynamic requirement for community interactions during Xenopus myogenesis.
2002,
Pubmed
,
Xenbase
Standley,
Uncommitted Xenopus blastula cells can be directed to uniform muscle gene expression by gradient interpretation and a community effect.
2003,
Pubmed
,
Xenbase
Stennard,
Differential expression of VegT and Antipodean protein isoforms in Xenopus.
1999,
Pubmed
,
Xenbase
Strumpf,
Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst.
2005,
Pubmed
Sugiyama,
Experience-dependent transfer of Otx2 homeoprotein into the visual cortex activates postnatal plasticity.
2008,
Pubmed
Tada,
Analysis of competence and of Brachyury autoinduction by use of hormone-inducible Xbra.
1997,
Pubmed
,
Xenbase
Wilson,
The T-box family.
2002,
Pubmed