Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Physiol
2009 Feb 01;5873:521-9. doi: 10.1113/jphysiol.2008.161562.
Show Gene links
Show Anatomy links
NKCC1 (SLC12a2) induces a secondary axis in Xenopus laevis embryos independently of its co-transporter function.
Walters ZS
,
Haworth KE
,
Latinkic BV
.
???displayArticle.abstract??? NKCC1 is a broadly expressed Na(+)-K(+)-Cl(-) co-transporter involved in regulation of ion flux across the cell membrane and in regulating cell volume. Whilst much is known about the co-transporter activity of NKCC1 and its regulation by protein kinases and phosphatases, little is known about the activities of NKCC1 that are co-transporter independent. In this report we show that over-expression of NKCC1 in embryos of Xenopus laevis induces secondary axes, independently of its co-transporter activity. In addition, over-expression of NKCC1 results in the formation of neural tissue in ectodermal explants. We also show that NKCC1 is expressed broadly but non-uniformly in embryos of Xenopus laevis and Xenopus tropicalis, with prominent expression in the notochord, nervous system and stomach. These results provide insights into an additional, previously unreported activity of NKCCl.
Carnac,
The homeobox gene Siamois is a target of the Wnt dorsalisation pathway and triggers organiser activity in the absence of mesoderm.
1996, Pubmed,
Xenbase
Carnac,
The homeobox gene Siamois is a target of the Wnt dorsalisation pathway and triggers organiser activity in the absence of mesoderm.
1996,
Pubmed
,
Xenbase
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Darman,
Modulation of ion transport by direct targeting of protein phosphatase type 1 to the Na-K-Cl cotransporter.
2001,
Pubmed
De Robertis,
Dorsal-ventral patterning and neural induction in Xenopus embryos.
2004,
Pubmed
,
Xenbase
Delpire,
Deafness and imbalance associated with inactivation of the secretory Na-K-2Cl co-transporter.
1999,
Pubmed
Dixon,
Mutation of the Na-K-Cl co-transporter gene Slc12a2 results in deafness in mice.
1999,
Pubmed
Flatman,
Cotransporters, WNKs and hypertension: an update.
2008,
Pubmed
,
Xenbase
Flatman,
Regulation of Na-K-2Cl cotransport by phosphorylation and protein-protein interactions.
2002,
Pubmed
Gamba,
Primary structure and functional expression of a cDNA encoding the thiazide-sensitive, electroneutral sodium-chloride cotransporter.
1993,
Pubmed
,
Xenbase
Gamba,
Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney.
1994,
Pubmed
,
Xenbase
Gamba,
Molecular physiology and pathophysiology of electroneutral cation-chloride cotransporters.
2005,
Pubmed
Gammill,
Identification of otx2 target genes and restrictions in ectodermal competence during Xenopus cement gland formation.
1997,
Pubmed
,
Xenbase
Garg,
Effect of the Na-K-2Cl cotransporter NKCC1 on systemic blood pressure and smooth muscle tone.
2007,
Pubmed
Gilchrist,
Defining a large set of full-length clones from a Xenopus tropicalis EST project.
2004,
Pubmed
,
Xenbase
Giménez,
Molecular mechanisms and regulation of furosemide-sensitive Na-K-Cl cotransporters.
2006,
Pubmed
Hebert,
Molecular physiology of cation-coupled Cl- cotransport: the SLC12 family.
2004,
Pubmed
Kanaka,
The differential expression patterns of messenger RNAs encoding K-Cl cotransporters (KCC1,2) and Na-K-2Cl cotransporter (NKCC1) in the rat nervous system.
2001,
Pubmed
Kintner,
Monoclonal antibodies identify blastemal cells derived from dedifferentiating limb regeneration.
,
Pubmed
,
Xenbase
Kuroda,
Default neural induction: neuralization of dissociated Xenopus cells is mediated by Ras/MAPK activation.
2005,
Pubmed
,
Xenbase
Liedtke,
Activation of NKCC1 by hyperosmotic stress in human tracheal epithelial cells involves PKC-delta and ERK.
2002,
Pubmed
Lytle,
Activation of the avian erythrocyte Na-K-Cl cotransport protein by cell shrinkage, cAMP, fluoride, and calyculin-A involves phosphorylation at common sites.
1997,
Pubmed
Mercado,
Functional and molecular characterization of the K-Cl cotransporter of Xenopus laevis oocytes.
2001,
Pubmed
,
Xenbase
Molenaar,
XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos.
1996,
Pubmed
,
Xenbase
Panet,
Na+/K+/Cl- cotransporter activates MAP-kinase cascade downstream to protein kinase C, and upstream to MEK.
2006,
Pubmed
Payne,
Molecular characterization of the epithelial Na-K-Cl cotransporter isoforms.
1995,
Pubmed
Piechotta,
Cation chloride cotransporters interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1).
2002,
Pubmed
Richter,
Gene expression in the embryonic nervous system of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Sater,
Evidence for antagonism of BMP-4 signals by MAP kinase during Xenopus axis determination and neural specification.
2003,
Pubmed
,
Xenbase
Suvitayavat,
Characterization of the proteins of the intestinal Na(+)-K(+)-2Cl- cotransporter.
1994,
Pubmed
Xu,
Molecular cloning and functional expression of the bumetanide-sensitive Na-K-Cl cotransporter.
1994,
Pubmed
Zimmerman,
The Spemann organizer signal noggin binds and inactivates bone morphogenetic protein 4.
1996,
Pubmed
,
Xenbase
