Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Plant Physiol
2009 Jan 01;1491:297-305. doi: 10.1104/pp.108.128132.
Show Gene links
Show Anatomy links
OsFRDL1 is a citrate transporter required for efficient translocation of iron in rice.
Yokosho K
,
Yamaji N
,
Ueno D
,
Mitani N
,
Ma JF
.
???displayArticle.abstract???
Multidrug and toxic compound extrusion (MATE) transporters represent a large family in plants, but their functions are poorly understood. Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1), the closest homolog of barley (Hordeum vulgare) HvAACT1 (aluminum [Al]-activated citrate transporter 1), in terms of metal stress (iron [Fe] deficiency and Al toxicity). This gene was mainly expressed in the roots and the expression level was not affected by either Fe deficiency or Al toxicity. Knockout of this gene resulted in leaf chlorosis, lower leaf Fe concentration, higher accumulation of zinc and manganese concentration in the leaves, and precipitation of Fe in the root's stele. The concentration of citrate and ferric iron in the xylem sap was lower in the knockout line compared to the wild-type rice. Heterologous expression of OsFRDL1 in Xenopus oocytes showed transport activity for citrate. Immunostaining showed that OsFRDL1 was localized at the pericycle cells of the roots. On the other hand, there was no difference in the Al-induced secretion of citrate from the roots between the knockout line and the wild-type rice. Taken together, our results indicate that OsFRDL1 is a citrate transporter localized at the pericycle cells, which is necessary for efficient translocation of Fe to the shoot as a Fe-citrate complex.
Bughio,
Cloning an iron-regulated metal transporter from rice.
2002, Pubmed
Bughio,
Cloning an iron-regulated metal transporter from rice.
2002,
Pubmed
Debeaujon,
The TRANSPARENT TESTA12 gene of Arabidopsis encodes a multidrug secondary transporter-like protein required for flavonoid sequestration in vacuoles of the seed coat endothelium.
2001,
Pubmed
Delhaize,
Aluminum Tolerance in Wheat (Triticum aestivum L.) (II. Aluminum-Stimulated Excretion of Malic Acid from Root Apices).
1993,
Pubmed
Delhaize,
The roles of organic anion permeases in aluminium resistance and mineral nutrition.
2007,
Pubmed
Diener,
Arabidopsis ALF5, a multidrug efflux transporter gene family member, confers resistance to toxins.
2001,
Pubmed
Durrett,
The FRD3-mediated efflux of citrate into the root vasculature is necessary for efficient iron translocation.
2007,
Pubmed
,
Xenbase
Eide,
A novel iron-regulated metal transporter from plants identified by functional expression in yeast.
1996,
Pubmed
Furukawa,
An aluminum-activated citrate transporter in barley.
2007,
Pubmed
,
Xenbase
Green,
FRD3 controls iron localization in Arabidopsis.
2004,
Pubmed
Ishimaru,
Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+.
2006,
Pubmed
Korshunova,
The IRT1 protein from Arabidopsis thaliana is a metal transporter with a broad substrate range.
1999,
Pubmed
Lahner,
Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana.
2003,
Pubmed
Li,
Functional cloning and characterization of a plant efflux carrier for multidrug and heavy metal detoxification.
2002,
Pubmed
Ma,
Response of rice to Al stress and identification of quantitative trait Loci for Al tolerance.
2002,
Pubmed
Ma,
Aluminium tolerance in plants and the complexing role of organic acids.
2001,
Pubmed
Ma,
Aluminum targets elongating cells by reducing cell wall extensibility in wheat roots.
2004,
Pubmed
Ma,
A silicon transporter in rice.
2006,
Pubmed
Magalhaes,
A gene in the multidrug and toxic compound extrusion (MATE) family confers aluminum tolerance in sorghum.
2007,
Pubmed
Marinova,
The Arabidopsis MATE transporter TT12 acts as a vacuolar flavonoid/H+ -antiporter active in proanthocyanidin-accumulating cells of the seed coat.
2007,
Pubmed
Morita,
NorM, a putative multidrug efflux protein, of Vibrio parahaemolyticus and its homolog in Escherichia coli.
1998,
Pubmed
Omote,
The MATE proteins as fundamental transporters of metabolic and xenobiotic organic cations.
2006,
Pubmed
Otani,
Characterization of vacuolar transport of the endogenous alkaloid berberine in Coptis japonica.
2005,
Pubmed
Rogers,
FRD3, a member of the multidrug and toxin efflux family, controls iron deficiency responses in Arabidopsis.
2002,
Pubmed
Tiffin,
Translocation of iron citrate and phosphorus in xylem exudate of soybean.
1970,
Pubmed
Yamaji,
Spatial distribution and temporal variation of the rice silicon transporter Lsi1.
2007,
Pubmed
Yazaki,
Transporters of secondary metabolites.
2005,
Pubmed
