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Int J Dev Biol
2008 Jan 01;527:999-1004. doi: 10.1387/ijdb.082586dc.
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Cloning and developmental expression of the soxB2 genes, sox14 and sox21, during Xenopus laevis embryogenesis.
Cunningham DD
,
Meng Z
,
Fritzsch B
,
Casey ES
.
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The Sox family of transcription factors is thought to regulate gene expression in a wide variety of developmental processes. Here we describe the cloning of the X. laevis orthologs of the SoxB2 family of transcription factors, sox14 and sox21. In situ hybridization revealed that sox14 expression is restricted to the hypothalamus, dorsal thalamus, the optic tectum, a region of the somatic motornucleus in the midbrain and hindbrain, the vestibular nuclei in the hindbrain and a discrete ventral domain in the developing spinal cord. In contrast to the limited expression domain of sox14, sox21 is found throughout the developing central nervous system, including the olfactory placodes, with strongest expression at the boundary between the midbrain and hindbrain.
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18956331
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Fig. 3. Spatio-temporal expression pattern of sox14. (A) RT-PCR analysis from oocytes and embryos to stage 38 as indicated across the top. ODC (lower panel) was used as the loading control. (B-I) In situ hybridization of sox14 at indicated stages. In all figures, anterior is to the left. (B) Lateral view; (C) dorsal view, anterior view in the inset. (D) Sagittal sections of stage 38, double in situ of sox14 and en2 in the inset. (E) Dissected brain from stage 38 embryo. (F-I) Transverse sections from stage 32 (I) and 38 (F-H) embryos. Symbols: bracket, expression in vestibular nuclei; black arrowhead, dorsal thalamus; open arrowhead, dorsal hindbrainsox14 positive cells; M, midbrain; hy, hypothalamus; and AM, abducens motornucleus; mhb, midbrainhindbrain barrier; OT, optic tectum; DT, dorsal thalamus; OM, oculomotor motornucleus; TM, trochlear motornucleus; IN, interneurons. Black bar equivalent to 100 μm.
Fig. 4. Spatio-temporal expression pattern of sox21. (A) RT-PCR analysis of embryos from oocyte to stage 36 as indicated across the top. ODC was used as the loading control. (B,C,F,J,N,P) In situ hybridization of embryos stained for sox21 at indicated stages and (F-M, O) for regional brain markers as indicated. (B,C, J-M, inset P) Anterior views. (N-P) Dorsal views. (F-I) Lateral views. (D,E) Transverse sections generated from a stage 31 embryo. Symbols: Black asterisks, midbrain-hindbrain boundary; f, forebrain; h, hindbrain; m, midbrain; hy, hypothalamus; n, notochord; op, olfactory placode; ov, otic vesicle.
Bachy,
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,
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,
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Bermingham,
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Bowles,
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2000,
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Clamp,
The Jalview Java alignment editor.
2004,
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De Martino,
sox30: a novel zebrafish sox gene expressed in a restricted manner at the midbrain-hindbrain boundary during neurogenesis.
1999,
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Guo,
Development of noradrenergic neurons in the zebrafish hindbrain requires BMP, FGF8, and the homeodomain protein soulless/Phox2a.
1999,
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Hargrave,
The HMG box transcription factor gene Sox14 marks a novel subset of ventral interneurons and is regulated by sonic hedgehog.
2000,
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Harland,
In situ hybridization: an improved whole-mount method for Xenopus embryos.
1991,
Pubmed
,
Xenbase
Hartenstein,
Early pattern of neuronal differentiation in the Xenopus embryonic brainstem and spinal cord.
1993,
Pubmed
,
Xenbase
Hemmati-Brivanlou,
Cephalic expression and molecular characterization of Xenopus En-2.
1991,
Pubmed
,
Xenbase
Hollemann,
The Xenopus homologue of the Drosophila gene tailless has a function in early eye development.
1998,
Pubmed
,
Xenbase
Kurrasch,
The neonatal ventromedial hypothalamus transcriptome reveals novel markers with spatially distinct patterning.
2007,
Pubmed
Regad,
The neural progenitor-specifying activity of FoxG1 is antagonistically regulated by CKI and FGF.
2007,
Pubmed
,
Xenbase
Rex,
cSox21 exhibits a complex and dynamic pattern of transcription during embryonic development of the chick central nervous system.
1997,
Pubmed
Sandberg,
Sox21 promotes the progression of vertebrate neurogenesis.
2005,
Pubmed
Stevanović,
SOX3 is an X-linked gene related to SRY.
1993,
Pubmed
Swofford,
Bias in phylogenetic estimation and its relevance to the choice between parsimony and likelihood methods.
2001,
Pubmed
Talikka,
Characterization of Xenopus Phox2a and Phox2b defines expression domains within the embryonic nervous system and early heart field.
2004,
Pubmed
,
Xenbase
Uchikawa,
Two distinct subgroups of Group B Sox genes for transcriptional activators and repressors: their expression during embryonic organogenesis of the chicken.
1999,
Pubmed
Wilson,
Mesodermal patterning by an inducer gradient depends on secondary cell-cell communication.
1994,
Pubmed
,
Xenbase
