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Mech Dev. March 1, 2009; 126 (3-4): 142-59.

Requirement of Wnt/beta-catenin signaling in pronephric kidney development.

Lyons JP , Miller RK , Zhou X , Weidinger G , Deroo T , Denayer T , Park JI , Ji H , Hong JY , Li A , Moon RT , Jones EA , Vleminckx K , Vize PD , McCrea PD .

The pronephric kidney controls water and electrolyte balance during early fish and amphibian embryogenesis. Many Wnt signaling components have been implicated in kidney development. Specifically, in Xenopus pronephric development as well as the murine metanephroi, the secreted glycoprotein Wnt-4 has been shown to be essential for renal tubule formation. Despite the importance of Wnt signals in kidney organogenesis, little is known of the definitive downstream signaling pathway(s) that mediate their effects. Here we report that inhibition of Wnt/beta-catenin signaling within the pronephric field of Xenopus results in significant losses to kidney epithelial tubulogenesis with little or no effect on adjoining axis or somite development. We find that the requirement for Wnt/beta-catenin signaling extends throughout the pronephric primordium and is essential for the development of proximal and distal tubules of the pronephros as well as for the development of the duct and glomus. Although less pronounced than effects upon later pronephric tubule differentiation, inhibition of the Wnt/beta-catenin pathway decreased expression of early pronephric mesenchymal markers indicating it is also needed in early pronephric patterning. We find that upstream inhibition of Wnt/beta-catenin signals in zebrafish likewise reduces pronephric epithelial tubulogenesis. We also find that exogenous activation of Wnt/beta-catenin signaling within the Xenopus pronephric field results in significant tubulogenic losses. Together, we propose Wnt/beta-catenin signaling is required for pronephric tubule, duct and glomus formation in Xenopus laevis, and this requirement is conserved in zebrafish pronephric tubule formation.

PubMed ID: 19100832
PMC ID: PMC2684468
Article link: Mech Dev.
Grant support: CA-16672 NCI NIH HHS , DE015355 NIDCR NIH HHS, DK082145 NIDDK NIH HHS , HD07325 NICHD NIH HHS , R01 (GM052112) NIGMS NIH HHS , R01 GM052112-09 NIGMS NIH HHS , R01 GM052112-10 NIGMS NIH HHS , F32 DK082145 NIDDK NIH HHS , R01 GM052112 NIGMS NIH HHS , T32 HD007325 NICHD NIH HHS , CA-16672 NCI NIH HHS , P30 CA016672 NCI NIH HHS , R01 GM052112 NIGMS NIH HHS , DK082145 NIDDK NIH HHS , R01 (GM052112) NIGMS NIH HHS , DE015355 NIDCR NIH HHS, R01 GM052112-09 NIGMS NIH HHS , T32 HD007325 NICHD NIH HHS , F32 DK082145 NIDDK NIH HHS , T32 DE015355 NIDCR NIH HHS, R01 GM052112-10 NIGMS NIH HHS , HD07325 NICHD NIH HHS

Genes referenced: hnf1b lef1 lhx1 nphs1 nr3c1 pax8 pcyt1a slc12a1 slc4a4 tbk1 wnt4 atp1a1

Aulehla, 2004, Pubmed[+]

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