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Development March 1, 2009; 136 (5): 729-38.

The non-methylated DNA-binding function of Kaiso is not required in early Xenopus laevis development.

Ruzov A , Savitskaya E , Hackett JA , Reddington JP , Prokhortchouk A , Madej MJ , Chekanov N , Li M , Dunican DS , Prokhortchouk E , Pennings S , Meehan RR .

Mammalian forms of the transcription repressor, Kaiso, can reportedly bind methylated DNA and non-methylated CTGCNA motifs. Here we compare the DNA-binding properties of Kaiso from frog, fish and chicken and demonstrate that only the methyl-CpG-binding function of Kaiso is evolutionarily conserved. We present several independent experimental lines of evidence that the phenotypic abnormalities associated with xKaiso-depleted Xenopus laevis embryos are independent of the putative CTGCNA-dependent DNA-binding function of xKaiso. Our analysis suggests that xKaiso does not play a role in the regulation of either xWnt11 or Siamois, key signalling molecules in the Wnt pathway during X. laevis gastrulation. The major phenotypic defects associated with xKaiso depletion are premature transcription activation before the mid-blastula transition and concomitant activation of a p53-dependent cell-death pathway.

PubMed ID: 19158185
PMC ID: PMC2685941
Article link: Development
Grant support: [+]
Genes referenced: casp7 casp9 ecd h4c4 id2 kmo sia1 tbx2 tp53 wnt11 wnt11b zbtb33

Article Images: [+] show captions
References [+] :
Allen, Solution structure of the nonmethyl-CpG-binding CXXC domain of the leukaemia-associated MLL histone methyltransferase. 2006, Pubmed

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