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XB-ART-39172
Dev Biol March 15, 2009; 327 (2): 386-98.

The physical state of fibronectin matrix differentially regulates morphogenetic movements in vivo.

Rozario T , Dzamba B , Weber GF , Davidson LA , DeSimone DW .


Abstract
This study demonstrates that proper spatiotemporal expression and the physical assembly state of fibronectin (FN) matrix play key roles in the regulation of morphogenetic cell movements in vivo. We examine the progressive assembly and 3D fibrillar organization of FN and its role in regulating cell and tissue movements in Xenopus embryos. Expression of the 70 kD N-terminal fragment of FN blocks FN fibril assembly at gastrulation but not initial FN binding to integrins at the cell surface. We find that fibrillar FN is necessary to maintain cell polarity through oriented cell division and to promote epiboly, possibly through maintenance of tissue-surface tension. In contrast, FN fibrils are dispensable for convergence and extension movements required for axis elongation. Closure of the migratory mesendodermal mantle was accelerated in the absence of a fibrillar matrix. Thus, the macromolecular assembly of FN matrices may constitute a general regulatory mechanism for coordination of distinct morphogenetic movements.

PubMed ID: 19138684
PMC ID: PMC2829434
Article link: Dev Biol
Grant support: [+]
Genes referenced: chrd.1 fn1
Antibodies: Ptk2 Ab2


Article Images: [+] show captions
References [+] :
Baneyx, Self-assembly of fibronectin into fibrillar networks underneath dipalmitoyl phosphatidylcholine monolayers: role of lipid matrix and tensile forces. 1999, Pubmed


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