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Am J Physiol Cell Physiol
2009 May 01;2965:C1003-14. doi: 10.1152/ajpcell.00474.2008.
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Inhibitory effects of polyunsaturated fatty acids on Kv4/KChIP potassium channels.
Boland LM
,
Drzewiecki MM
,
Timoney G
,
Casey E
.
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Kv4/K channel interacting protein (KChIP) potassium channels are a major class of rapidly inactivating K(+) channels in neurons and cardiac muscle. Modulation of Kv4/KChIP channels by polyunsaturated fatty acids (PUFAs) is important in the regulation of cellular excitability and the induction of activity-dependent synaptic plasticity. Using the Xenopus laevis oocyte expression system, we studied the inhibition by PUFAs of the peak outward K(+) current and the accompanying increase in the rate of current inactivation of rKv4.2/rKChIP1b. Inhibitory effects do not depend on KChIP coexpression since Kv4.2 channels lacking an NH(2)-terminal KChIP association region were substantially inhibited by PUFAs and showed strong kinetic modulation. PUFAs accelerated both the fast and slow time constants that describe the kinetics of Kv4/KChIP inactivation. The time course of entry into closed inactivated states was facilitated by PUFAs, but steady-state inactivation and recovery from inactivation were unaltered. PUFA inhibition of Kv4/KChIP current was not use dependent. The concentration-response relationship for arachidonic acid (AA) inhibition of Kv4/KChIP channels mimicked that for activation of TRAAK channels. Internal serum albumin largely prevents the inhibitory effects of externally applied AA, and the membrane-impermeant AA-CoA is inactive when applied externally. Overall, our data suggest that PUFAs inhibit Kv4/KChIP channels by facilitating inactivation from open and closed gating states and that access of the fatty acid to the internal leaflet of the membrane is important. These results improve our understanding of the mechanisms for the inhibitory effects of PUFAs on Kv4/KChIP channel function.
Amarillo,
Ternary Kv4.2 channels recapitulate voltage-dependent inactivation kinetics of A-type K+ channels in cerebellar granule neurons.
2008, Pubmed
Amarillo,
Ternary Kv4.2 channels recapitulate voltage-dependent inactivation kinetics of A-type K+ channels in cerebellar granule neurons.
2008,
Pubmed
An,
Modulation of A-type potassium channels by a family of calcium sensors.
2000,
Pubmed
,
Xenbase
Angelova,
Oxidative modulation of the transient potassium current IA by intracellular arachidonic acid in rat CA1 pyramidal neurons.
2006,
Pubmed
Baldwin,
Characterization of a mammalian cDNA for an inactivating voltage-sensitive K+ channel.
1991,
Pubmed
,
Xenbase
Bazan,
Synaptic signaling by lipids in the life and death of neurons.
2005,
Pubmed
Beck,
Remodelling inactivation gating of Kv4 channels by KChIP1, a small-molecular-weight calcium-binding protein.
2002,
Pubmed
,
Xenbase
Bittner,
Oxidative downmodulation of the transient K-current IA by intracellular arachidonic acid in rat hippocampal neurons.
1999,
Pubmed
Boland,
Polyunsaturated fatty acid modulation of voltage-gated ion channels.
2008,
Pubmed
Boland,
Functional properties of a brain-specific NH2-terminally spliced modulator of Kv4 channels.
2003,
Pubmed
,
Xenbase
Bähring,
Conserved Kv4 N-terminal domain critical for effects of Kv channel-interacting protein 2.2 on channel expression and gating.
2001,
Pubmed
Bähring,
Kinetic analysis of open- and closed-state inactivation transitions in human Kv4.2 A-type potassium channels.
2001,
Pubmed
Colbert,
Arachidonic acid reciprocally alters the availability of transient and sustained dendritic K(+) channels in hippocampal CA1 pyramidal neurons.
1999,
Pubmed
Covarrubias,
The neuronal Kv4 channel complex.
2008,
Pubmed
Curry,
Crystal structure of human serum albumin complexed with fatty acid reveals an asymmetric distribution of binding sites.
1998,
Pubmed
Decher,
hKChIP2 is a functional modifier of hKv4.3 potassium channels: cloning and expression of a short hKChIP2 splice variant.
2001,
Pubmed
,
Xenbase
Deschênes,
Modulation of Kv4.3 current by accessory subunits.
2002,
Pubmed
Dixon,
Role of the Kv4.3 K+ channel in ventricular muscle. A molecular correlate for the transient outward current.
1996,
Pubmed
Dryer,
Arachidonic acid-sensitive A-currents and multiple Kv4 transcripts are expressed in chick ciliary ganglion neurons.
1998,
Pubmed
Farooqui,
Brain phospholipases A2: a perspective on the history.
2004,
Pubmed
Fink,
A neuronal two P domain K+ channel stimulated by arachidonic acid and polyunsaturated fatty acids.
1998,
Pubmed
,
Xenbase
Fujita,
Docosahexaenoic acid improves long-term potentiation attenuated by phospholipase A(2) inhibitor in rat hippocampal slices.
2001,
Pubmed
Gebauer,
N-type inactivation features of Kv4.2 channel gating.
2004,
Pubmed
Guo,
Functional consequences of elimination of i(to,f) and i(to,s): early afterdepolarizations, atrioventricular block, and ventricular arrhythmias in mice lacking Kv1.4 and expressing a dominant-negative Kv4 alpha subunit.
2000,
Pubmed
Hamilton,
Fatty acid interactions with proteins: what X-ray crystal and NMR solution structures tell us.
2004,
Pubmed
Hoffman,
K+ channel regulation of signal propagation in dendrites of hippocampal pyramidal neurons.
1997,
Pubmed
Holmqvist,
Kinetic modulation of Kv4-mediated A-current by arachidonic acid is dependent on potassium channel interacting proteins.
2001,
Pubmed
,
Xenbase
Jerng,
K+ channel inactivation mediated by the concerted action of the cytoplasmic N- and C-terminal domains.
1997,
Pubmed
,
Xenbase
Jerng,
Inactivation gating of Kv4 potassium channels: molecular interactions involving the inner vestibule of the pore.
1999,
Pubmed
,
Xenbase
Jerng,
Multiprotein assembly of Kv4.2, KChIP3 and DPP10 produces ternary channel complexes with ISA-like properties.
2005,
Pubmed
,
Xenbase
Johns,
Suppression of neuronal and cardiac transient outward currents by viral gene transfer of dominant-negative Kv4.2 constructs.
1997,
Pubmed
Johnston,
Dendritic potassium channels in hippocampal pyramidal neurons.
2000,
Pubmed
Kehl,
Eicosatetraynoic acid (ETYA), a non-metabolizable analogue of arachidonic acid, blocks the fast-inactivating potassium current of rat pituitary melanotrophs.
2001,
Pubmed
Keros,
Arachidonic acid inhibits transient potassium currents and broadens action potentials during electrographic seizures in hippocampal pyramidal and inhibitory interneurons.
1997,
Pubmed
Kim,
Potassium channels: newly found players in synaptic plasticity.
2008,
Pubmed
Leaf,
Interactions of n-3 fatty acids with ion channels in excitable tissues.
2002,
Pubmed
Lesage,
Cloning and expression of human TRAAK, a polyunsaturated fatty acids-activated and mechano-sensitive K(+) channel.
2000,
Pubmed
Liss,
Tuning pacemaker frequency of individual dopaminergic neurons by Kv4.3L and KChip3.1 transcription.
2001,
Pubmed
Lotshaw,
Biophysical, pharmacological, and functional characteristics of cloned and native mammalian two-pore domain K+ channels.
2007,
Pubmed
Maffie,
A novel DPP6 isoform (DPP6-E) can account for differences between neuronal and reconstituted A-type K(+) channels.
2009,
Pubmed
Magee,
A synaptically controlled, associative signal for Hebbian plasticity in hippocampal neurons.
1997,
Pubmed
Migliore,
Role of an A-type K+ conductance in the back-propagation of action potentials in the dendrites of hippocampal pyramidal neurons.
1999,
Pubmed
Nakamura,
Different effects of the Ca(2+)-binding protein, KChIP1, on two Kv4 subfamily members, Kv4.1 and Kv4.2.
2001,
Pubmed
,
Xenbase
Nishizaki,
Arachidonic acid as a messenger for the expression of long-term potentiation.
1999,
Pubmed
Oliver,
Functional conversion between A-type and delayed rectifier K+ channels by membrane lipids.
2004,
Pubmed
,
Xenbase
Pak,
mShal, a subfamily of A-type K+ channel cloned from mammalian brain.
1991,
Pubmed
Patel,
Regulation of Kv4.3 voltage-dependent gating kinetics by KChIP2 isoforms.
2004,
Pubmed
,
Xenbase
Piomelli,
A neuroscientist's guide to lipidomics.
2007,
Pubmed
Poling,
Time- and voltage-dependent block of delayed rectifier potassium channels by docosahexaenoic acid.
1995,
Pubmed
Radicke,
Functional modulation of the transient outward current Ito by KCNE beta-subunits and regional distribution in human non-failing and failing hearts.
2006,
Pubmed
Ramakers,
A postsynaptic transient K(+) current modulated by arachidonic acid regulates synaptic integration and threshold for LTP induction in hippocampal pyramidal cells.
2002,
Pubmed
Ren,
Transmembrane interaction mediates complex formation between peptidase homologues and Kv4 channels.
2005,
Pubmed
Rhodes,
KChIPs and Kv4 alpha subunits as integral components of A-type potassium channels in mammalian brain.
2004,
Pubmed
Rosati,
Regulation of KChIP2 potassium channel beta subunit gene expression underlies the gradient of transient outward current in canine and human ventricle.
2001,
Pubmed
,
Xenbase
Serôdio,
Identification of molecular components of A-type channels activating at subthreshold potentials.
1994,
Pubmed
,
Xenbase
Serôdio,
Cloning of a novel component of A-type K+ channels operating at subthreshold potentials with unique expression in heart and brain.
1996,
Pubmed
,
Xenbase
Smirnov,
Modulatory effects of arachidonic acid on the delayed rectifier K+ current in rat pulmonary arterial myocytes. Structural aspects and involvement of protein kinase C.
1996,
Pubmed
Sokolowski,
Identification and localization of an arachidonic acid-sensitive potassium channel in the cochlea.
2004,
Pubmed
Song,
Somatodendritic depolarization-activated potassium currents in rat neostriatal cholinergic interneurons are predominantly of the A type and attributable to coexpression of Kv4.2 and Kv4.1 subunits.
1998,
Pubmed
Sun,
Beta-subunit-dependent modulation of hSlo BK current by arachidonic acid.
2007,
Pubmed
,
Xenbase
Takimoto,
Species and tissue differences in the expression of DPPY splicing variants.
2006,
Pubmed
Taylor,
Potassium-evoked glutamate release liberates arachidonic acid from cortical neurons.
2002,
Pubmed
Tkatch,
Kv4.2 mRNA abundance and A-type K(+) current amplitude are linearly related in basal ganglia and basal forebrain neurons.
2000,
Pubmed
Villarroel,
Inhibition of the Kv4 (Shal) family of transient K+ currents by arachidonic acid.
1996,
Pubmed
,
Xenbase
Visentin,
Arachidonic acid-induced inhibition of microglial outward-rectifying K+ current.
1998,
Pubmed
Wolf,
Long-term potentiation requires activation of calcium-independent phospholipase A2.
1995,
Pubmed
Yeola,
Electrophysiological and pharmacological correspondence between Kv4.2 current and rat cardiac transient outward current.
1997,
Pubmed
Zagha,
DPP10 modulates Kv4-mediated A-type potassium channels.
2005,
Pubmed
Zhang,
minK-related peptide 1 associates with Kv4.2 and modulates its gating function: potential role as beta subunit of cardiac transient outward channel?
2001,
Pubmed
,
Xenbase
