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Dev Biol May 1, 2009; 329 (1): 130-9.

DeltaNp63 antagonizes p53 to regulate mesoderm induction in Xenopus laevis.

Barton CE , Tahinci E , Barbieri CE , Johnson KN , Hanson AJ , Jernigan KK , Chen TW , Lee E , Pietenpol JA .

p63, a homolog of the tumor suppressor p53, is critical for the development and maintenance of complex epithelia. The developmentally regulated p63 isoform, DeltaNp63, can act as a transcriptional repressor, but the link between the transcriptional functions of p63 and its biological roles is unclear. Based on our initial finding that the mesoderm-inducing factor activin A is suppressed by DeltaNp63 in human keratinocytes, we investigated the role of DeltaNp63 in regulating mesoderm induction during early Xenopus laevis development. We find that down-regulation of DeltaNp63 by morpholino injection in the early Xenopus embryo potentiates mesoderm formation whereas ectopic expression of DeltaNp63 inhibits mesoderm formation. Furthermore, we show that mesodermal induction after down-regulation of DeltaNp63 is dependent on p53. We propose that a key function for p63 in defining a squamous epithelial phenotype is to actively suppress mesodermal cell fates during early development. Collectively, we show that there is a distinct requirement for different p53 family members during the development of both mesodermal and ectodermal tissues. These findings have implications for the role of p63 and p53 in both development and tumorigenesis of human epithelia.

PubMed ID: 19272371
PMC ID: PMC2690611
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: chrd.1 gal.2 gsc inhba krt12.4 odc1 tbx2 tbxt tp53 tp63 wnt8a
Antibodies: Tp63 Ab1
Morpholinos: tp63 MO1 tp63 MO2

Article Images: [+] show captions
References [+] :
Agius, Endodermal Nodal-related signals and mesoderm induction in Xenopus. 2000, Pubmed, Xenbase