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XB-ART-39588
Development May 1, 2009; 136 (10): 1707-15.

Defining retinal progenitor cell competence in Xenopus laevis by clonal analysis.

Wong LL , Rapaport DH .


Abstract
Extrinsic cues and intrinsic competence act in concert for cell fate determination in the developing vertebrate retina. However, what controls competence and how precise is the control are largely unknown. We studied the regulation of competence by examining the order in which individual retinal progenitor cells (RPCs) generate daughters. Experiments were performed in Xenopus laevis, whose full complement of retinal cells is formed in 2 days. We lineage-labeled RPCs at the optic vesicle stage. Subsequently we administered a cell cycle marker, 5-bromodeoxyuridine (BrdU) at early, middle or late periods of retinogenesis. Under these conditions, and in this animal, BrdU is not cleared by the time of analysis, allowing cumulative labeling. All retinal cell types were generated throughout nearly the entire retinogenesis period. When we examined the order that individual RPCs generated daughters, we discovered a regular and consistent sequence according to phenotype: RGC, Ho, CPr, RPr, Am, BP, MG. The precision of the order between the clones supports a model in which RPCs proceed through stepwise changes in competence to make each cell type, and do so unidirectionally. Because every cell type can be generated simultaneously within the same retinal environment, the change in RPC competence is likely to be autonomous.

PubMed ID: 19395642
PMC ID: PMC2673759
Article link: Development
Grant support: [+]
Genes referenced: rho tbx2 zic1
Antibodies: BrdU Ab5 Rho Ab5


Article Images: [+] show captions
References [+] :
Alexiades, Subsets of retinal progenitors display temporally regulated and distinct biases in the fates of their progeny. 1997, Pubmed


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