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Dev Dyn June 1, 2009; 238 (6): 1398-46.
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Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes.

Khokha MK , Krylov V , Reilly MJ , Gall JG , Bhattacharya D , Cheung CY , Kaufman S , Lam DK , Macha J , Ngo C , Prakash N , Schmidt P , Tlapakova T , Trivedi T , Tumova L , Abu-Daya A , Geach T , Vendrell E , Ironfield H , Sinzelle L , Sater AK , Wells DE , Harland RM , Zimmerman LB .

Pilot forward genetic screens in Xenopus tropicalis have isolated over 60 recessive mutations. Here we present a simple method for mapping mutations to chromosomes using gynogenesis and centromeric markers. When coupled with available genomic resources, gross mapping facilitates evaluation of candidate genes as well as higher resolution linkage studies. Using gynogenesis, we have mapped the genetic locations of the 10 X. tropicalis centromeres, and performed fluorescence in situ hybridization to validate these locations cytologically. We demonstrate the use of this very small set of centromeric markers to map mutations efficiently to specific chromosomes. Developmental Dynamics 238:1398-1406, 2009. (c) 2009 Wiley-Liss, Inc.

PubMed ID: 19441086
PMC ID: PMC2962985
Article link: Dev Dyn
Grant support: [+]

Species referenced: Xenopus tropicalis
Genes referenced: epb41 ezh1 ezh2 fbxl7 gemin5 mast3 mat1a naif1 olig3 stat4 znf423

Article Images: [+] show captions
References [+] :
Amaya, Frog genetics: Xenopus tropicalis jumps into the future. 1998, Pubmed, Xenbase