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XB-ART-39723
J Biol Chem July 24, 2009; 284 (30): 19982-92.

Participation of Xenopus Elr-type proteins in vegetal mRNA localization during oogenesis.

Arthur PK , Claussen M , Koch S , Tarbashevich K , Jahn O , Pieler T .


Abstract
Directional transport of specific mRNAs is of primary biological relevance. In Xenopus oocytes, mRNA localization to the vegetal pole is important for germ layer formation and germ cell development. Using a biochemical approach, we identified Xenopus Elr-type proteins, homologs of the Hu/ELAV proteins, as novel components of the vegetal mRNA localization machinery. They bind specifically to the localization elements of several different vegetally localizing Xenopus mRNAs, and they are part of one RNP together with other localization proteins, such as Vg1RBP and XStaufen 1. Blocking Elr-type protein binding by either localization element mutagenesis or antisense morpholino oligonucleotide-mediated masking of their target RNA structures, as well as overexpression of wild type and mutant ElrB proteins, interferes with vegetal localization in Xenopus oocytes.

PubMed ID: 19458392
PMC ID: PMC2740424
Article link: J Biol Chem

Genes referenced: dnd1 elavl1 elavl2 fam102a hnrnpab igf2bp3 mbd2 myc rrad tbx2
Morpholinos: dnd1 MO2 dnd1 MO3 dnd1 MO4 dnd1 MO5 fam102a MO1 mbd2 MO1 rrad MO1


Article Images: [+] show captions
References [+] :
Alarcón, RNA anchoring in the vegetal cortex of the Xenopus oocyte. 2001, Pubmed, Xenbase


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