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Biophys J May 20, 2009; 96 (10): 4319-25.

Influence of lamin A on the mechanical properties of amphibian oocyte nuclei measured by atomic force microscopy.

Schäpe J , Prausse S , Radmacher M , Stick R .

The nuclear lamina is part of the nuclear envelope (NE). Lamin filaments provide the nucleus with mechanical stability and are involved in many nuclear activities. The functional importance of these proteins is highlighted by mutations in lamin genes, which cause a variety of human diseases (laminopathies). Here we describe a method that allows one to quantify the contribution of lamin A protein to the mechanical properties of the NE. Lamin A is ectopically expressed in Xenopus oocytes, where it is incorporated into the NE of the oocyte nucleus, giving rise to a prominent lamina layer at the inner nuclear membrane. Nuclei are then isolated and probed by atomic force microscopy. From the resulting force curves, stiffness values are calculated and compared with those of control nuclei. Expression of lamin A significantly increases the stiffness of oocyte nuclei in a concentration-dependent manner. Since chromatin adds negligibly to nuclear mechanics in these giant nuclei, this method allows one to measure the contribution of individual NE components to nuclear mechanics.

PubMed ID: 19450502
PMC ID: PMC2712154
Article link: Biophys J

Species referenced: Xenopus laevis
Genes referenced: lmna
GO keywords: nuclear envelope

Disease Ontology terms: cardiomyopathy [+]

Article Images: [+] show captions
References [+] :
Aebi, The nuclear lamina is a meshwork of intermediate-type filaments. 1986, Pubmed, Xenbase