Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Development
2009 Jun 01;13612:2111-9. doi: 10.1242/dev.035089.
Show Gene links
Show Anatomy links
A shift from kinesin 5-dependent metaphase spindle function during preimplantation development in mouse.
Fitzharris G
.
???displayArticle.abstract???
Microtubules within meiotic and mitotic spindles continually move towards spindle poles in a process termed poleward flux, which is essential for spindle integrity and faithful chromosome segregation. Kinesin 5 is a longstanding candidate for a molecular motor that might drive poleward flux, and has been shown to drive flux and to be necessary for spindle bipolarity in Xenopus egg extracts. However, kinesin 5 is not necessary for poleward flux or for maintaining metaphase spindle bipolarity in intact mammalian cells, and the reason for the different results in these systems is unknown. The experiments presented here test the hypothesis that these results might reflect developmental differences in spindle function by examining the role of kinesin 5 in mouse eggs and preimplantation embryos. In contrast to cultured somatic cells, poleward flux in mouse eggs is critically dependent upon kinesin 5. Inhibition of poleward flux leads to spindle shortening as a result of continued microtubule depolymerisation at the pole, and eventual loss of spindle bipolarity. Spindle bipolarity is also dependent upon kinesin 5 during the first three embryonic cleavages, but becomes kinesin 5-independent in the majority of spindles by the blastocyst stage. This switch occurs asynchronously in different blastomeres but is independent of clonal cell heritage and of whether the blastomere is within the inner cell mass or the trophoectoderm. These experiments reveal a novel developmental switch in the requirements for spindle function and chromosome segregation during preimplantation development.
Abumuslimov,
[An electron microscopic study of centriole and centrosome morphogenesis in the early development of the mouse].
1994, Pubmed
Abumuslimov,
[An electron microscopic study of centriole and centrosome morphogenesis in the early development of the mouse].
1994,
Pubmed
Blangy,
Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo.
1995,
Pubmed
,
Xenbase
Brust-Mascher,
Kinesin-5-dependent poleward flux and spindle length control in Drosophila embryo mitosis.
2009,
Pubmed
Burbank,
A new method reveals microtubule minus ends throughout the meiotic spindle.
2006,
Pubmed
,
Xenbase
Burbank,
Slide-and-cluster models for spindle assembly.
2007,
Pubmed
,
Xenbase
Calarco-Gillam,
Centrosome development in early mouse embryos as defined by an autoantibody against pericentriolar material.
1983,
Pubmed
Cameron,
Kinesin 5-independent poleward flux of kinetochore microtubules in PtK1 cells.
2006,
Pubmed
Cimini,
Aurora kinase promotes turnover of kinetochore microtubules to reduce chromosome segregation errors.
2006,
Pubmed
Ferenz,
Prophase microtubule arrays undergo flux-like behavior in mammalian cells.
2007,
Pubmed
Gaglio,
Opposing motor activities are required for the organization of the mammalian mitotic spindle pole.
1996,
Pubmed
,
Xenbase
Ganem,
Functional roles of poleward microtubule flux during mitosis.
2006,
Pubmed
Ganem,
Efficient mitosis in human cells lacking poleward microtubule flux.
2005,
Pubmed
Goshima,
Length control of the metaphase spindle.
2005,
Pubmed
Groen,
A novel small-molecule inhibitor reveals a possible role of kinesin-5 in anastral spindle-pole assembly.
2008,
Pubmed
,
Xenbase
Gueth-Hallonet,
gamma-Tubulin is present in acentriolar MTOCs during early mouse development.
1993,
Pubmed
Kapitein,
The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks.
2005,
Pubmed
,
Xenbase
Kapoor,
Eg5 is static in bipolar spindles relative to tubulin: evidence for a static spindle matrix.
2001,
Pubmed
,
Xenbase
Kapoor,
Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5.
2000,
Pubmed
,
Xenbase
Kashina,
A bipolar kinesin.
1996,
Pubmed
Lawitts,
Culture of preimplantation embryos.
1993,
Pubmed
Mailhes,
Transient exposure to the Eg5 kinesin inhibitor monastrol leads to syntelic orientation of chromosomes and aneuploidy in mouse oocytes.
2004,
Pubmed
Maliga,
Small-molecule and mutational analysis of allosteric Eg5 inhibition by monastrol.
2006,
Pubmed
Maliga,
Evidence that monastrol is an allosteric inhibitor of the mitotic kinesin Eg5.
2002,
Pubmed
,
Xenbase
Margolis,
Microtubule treadmills--possible molecular machinery.
1981,
Pubmed
Mayer,
Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen.
1999,
Pubmed
,
Xenbase
Mitchison,
Sites of microtubule assembly and disassembly in the mitotic spindle.
1986,
Pubmed
Mitchison,
Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence.
1989,
Pubmed
Miyamoto,
The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles.
2004,
Pubmed
,
Xenbase
Mountain,
The kinesin-related protein, HSET, opposes the activity of Eg5 and cross-links microtubules in the mammalian mitotic spindle.
1999,
Pubmed
,
Xenbase
Ohi,
Nonredundant functions of Kinesin-13s during meiotic spindle assembly.
2007,
Pubmed
,
Xenbase
Patterson,
A photoactivatable GFP for selective photolabeling of proteins and cells.
2002,
Pubmed
Piotrowska,
Blastomeres arising from the first cleavage division have distinguishable fates in normal mouse development.
2001,
Pubmed
Rogers,
Spindle microtubules in flux.
2005,
Pubmed
Sawin,
Mitotic spindle organization by a plus-end-directed microtubule motor.
1992,
Pubmed
,
Xenbase
Sawin,
Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle.
1995,
Pubmed
,
Xenbase
Schuh,
Self-organization of MTOCs replaces centrosome function during acentrosomal spindle assembly in live mouse oocytes.
2007,
Pubmed
Sharp,
The bipolar kinesin, KLP61F, cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles.
1999,
Pubmed
Tulu,
Peripheral, non-centrosome-associated microtubules contribute to spindle formation in centrosome-containing cells.
2003,
Pubmed
Uteng,
Poleward transport of Eg5 by dynein-dynactin in Xenopus laevis egg extract spindles.
2008,
Pubmed
,
Xenbase
Yang,
Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle.
2008,
Pubmed
,
Xenbase
Yang,
Architectural dynamics of the meiotic spindle revealed by single-fluorophore imaging.
2007,
Pubmed
,
Xenbase
van den Wildenberg,
The homotetrameric kinesin-5 KLP61F preferentially crosslinks microtubules into antiparallel orientations.
2008,
Pubmed
