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Dev Dyn July 1, 2009; 238 (7): 1727-43.

Transgenesis in Xenopus using the Sleeping Beauty transposon system.

Yergeau DA , Johnson Hamlet MR , Kuliyev E , Zhu H , Doherty JR , Archer TD , Subhawong AP , Valentine MB , Kelley CM , Mead PE .

Transposon-based integration systems have been widely used for genetic manipulation of invertebrate and plant model systems. In the past decade, these powerful tools have begun to be used in vertebrates for transgenesis, insertional mutagenesis, and gene therapy applications. Sleeping Beauty (SB) is a member of Tc1/mariner class of transposases and is derived from an inactive form of the gene isolated from Atlantic salmon. SB has been used extensively in human cell lines and in whole animal vertebrate model systems such as the mouse, rat, and zebrafish. In this study, we describe the use of SB in the diploid frog Xenopus tropicalis to generate stable transgenic lines. SB transposon transgenes integrate into the X. tropicalis genome by a noncanonical process and are passed through the germline. We compare the activity of SB in this model organism with that of Tol2, a hAT (hobo, Ac1, TAM)-like transposon system.

PubMed ID: 19517568
PMC ID: PMC2848081
Article link: Dev Dyn
Grant support: 5R25CA023944 NCI NIH HHS , R01 HD042294 NICHD NIH HHS , R01 HD042294-01 NICHD NIH HHS , R01 HD042294-01S1 NICHD NIH HHS , R01 HD042294-02 NICHD NIH HHS , R01 HD042294-03 NICHD NIH HHS , R01 HD042294-04 NICHD NIH HHS , R01 HD042294-05 NICHD NIH HHS , R01 MH079381-01A2 NIMH NIH HHS, R01 MH079381 NIMH NIH HHS, R25 CA023944 NCI NIH HHS

Genes referenced: actl6a kidins220 myh3 tcim

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Amaya, 1998, Pubmed, Xenbase [+]

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