XB-ART-40042
Cell Mol Life Sci
2009 Oct 01;6620:3263-76. doi: 10.1007/s00018-009-0084-5.
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Predominant regulators of tubulin monomer-polymer partitioning and their implication for cell polarization.
Holmfeldt P
,
Sellin ME
,
Gullberg M
.
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The microtubule-system organizes the cytoplasm during interphase and segregates condensed chromosomes during mitosis. Four unrelated conserved proteins, XMAP215/Dis1/TOGp, MCAK, MAP4 and Op18/stathmin, have all been implicated as predominant regulators of tubulin monomer-polymer partitioning in animal cells. However, while studies employing the Xenopus egg extract model system indicate that the partitioning is largely governed by the counteractive activities of XMAP215 and MCAK, studies of human cell lines indicate that MAP4 and Op18 are the predominant regulators of the interphase microtubule-array. Here, we review functional interplay of these proteins during interphase and mitosis in various cell model systems. We also review the evidence that MAP4 and Op18 have interphase-specific, counteractive and phosphorylation-inactivated activities that govern tubulin subunit partitioning in many mammalian cell types. Finally, we discuss evidence indicating that partitioning regulation by MAP4 and Op18 may be of significance to establish cell polarity.
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Species referenced: Xenopus
Genes referenced: ckap5 kif2c map4 stmn1
References [+] :
Akhmanova,
Tracking the ends: a dynamic protein network controls the fate of microtubule tips.
2008, Pubmed
Akhmanova, Tracking the ends: a dynamic protein network controls the fate of microtubule tips. 2008, Pubmed
Al-Bassam, Stu2p binds tubulin and undergoes an open-to-closed conformational change. 2006, Pubmed
Al-Bassam, Crystal structure of a TOG domain: conserved features of XMAP215/Dis1-family TOG domains and implications for tubulin binding. 2007, Pubmed , Xenbase
Andersen, Spindle assembly and the art of regulating microtubule dynamics by MAPs and Stathmin/Op18. 2000, Pubmed
Andersen, Mitotic chromatin regulates phosphorylation of Stathmin/Op18. 1997, Pubmed , Xenbase
Andrews, Aurora B regulates MCAK at the mitotic centromere. 2004, Pubmed
Antonsson, Differences in phosphorylation of human and chicken stathmin by MAP kinase. 2001, Pubmed
Baldassarre, p27(Kip1)-stathmin interaction influences sarcoma cell migration and invasion. 2005, Pubmed
Belletti, Stathmin activity influences sarcoma cell shape, motility, and metastatic potential. 2008, Pubmed
Belmont, Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules. 1996, Pubmed , Xenbase
Bièche, Expression of stathmin family genes in human tissues: non-neural-restricted expression for SCLIP. 2003, Pubmed
Borghese, Systematic analysis of the transcriptional switch inducing migration of border cells. 2006, Pubmed
Brattsand, Cell-cycle-regulated phosphorylation of oncoprotein 18 on Ser16, Ser25 and Ser38. 1994, Pubmed
Brouhard, XMAP215 is a processive microtubule polymerase. 2008, Pubmed , Xenbase
Budde, Regulation of Op18 during spindle assembly in Xenopus egg extracts. 2001, Pubmed , Xenbase
Bulinski, Widespread distribution of a 210,000 mol wt microtubule-associated protein in cells and tissues of primates. 1980, Pubmed
Bulinski, Overexpression of MAP4 inhibits organelle motility and trafficking in vivo. 1997, Pubmed
Böhm, Mammalian homologues of C. elegans PAR-1 are asymmetrically localized in epithelial cells and may influence their polarity. 1997, Pubmed
Cantrell, T-cell antigen receptor signal transduction. 2002, Pubmed
Cassimeris, TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly. 2004, Pubmed
Cassimeris, XMAP215 is a long thin molecule that does not increase microtubule stiffness. 2001, Pubmed , Xenbase
Cassimeris, The oncoprotein 18/stathmin family of microtubule destabilizers. 2002, Pubmed
Caviston, Microtubule motors at the intersection of trafficking and transport. 2006, Pubmed
Cha, XMAP230 is required for normal spindle assembly in vivo and in vitro. 1999, Pubmed , Xenbase
Cha, XMAP230 is required for the assembly and organization of acetylated microtubules and spindles in Xenopus oocytes and eggs. 1998, Pubmed , Xenbase
Chapin, Differential expression of alternatively spliced forms of MAP4: a repertoire of structurally different microtubule-binding domains. 1995, Pubmed
Cleveland, Autoregulated instability of tubulin mRNAs: a novel eukaryotic regulatory mechanism. 1988, Pubmed
Combs, Recruitment of dynein to the Jurkat immunological synapse. 2006, Pubmed
Curmi, Stathmin and its phosphoprotein family: general properties, biochemical and functional interaction with tubulin. 1999, Pubmed
Curmi, The stathmin/tubulin interaction in vitro. 1997, Pubmed
Daub, Rac/Cdc42 and p65PAK regulate the microtubule-destabilizing protein stathmin through phosphorylation at serine 16. 2001, Pubmed
Desai, Kin I kinesins are microtubule-destabilizing enzymes. 1999, Pubmed , Xenbase
Desai, Microtubule polymerization dynamics. 1997, Pubmed
Drewes, MAPs, MARKs and microtubule dynamics. 1998, Pubmed
Drewes, MARK, a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption. 1997, Pubmed
Ebneth, Phosphorylation of MAP2c and MAP4 by MARK kinases leads to the destabilization of microtubules in cells. 1999, Pubmed
Etienne-Manneville, Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCzeta. 2001, Pubmed
Fletcher, Drosophila stathmin is required to maintain tubulin pools. 2007, Pubmed
Gadea, Aurora B is required for mitotic chromatin-induced phosphorylation of Op18/Stathmin. 2006, Pubmed , Xenbase
Ganem, Efficient mitosis in human cells lacking poleward microtubule flux. 2005, Pubmed
Gard, A microtubule-associated protein from Xenopus eggs that specifically promotes assembly at the plus-end. 1987, Pubmed , Xenbase
Gergely, The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells. 2003, Pubmed
Gigant, The 4 A X-ray structure of a tubulin:stathmin-like domain complex. 2000, Pubmed
Gomez, T cell activation and the cytoskeleton: you can't have one without the other. 2008, Pubmed
Gradin, Regulation of microtubule dynamics by extracellular signals: cAMP-dependent protein kinase switches off the activity of oncoprotein 18 in intact cells. 1998, Pubmed
Guo, par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed. 1995, Pubmed
Gustke, Domains of tau protein and interactions with microtubules. 1994, Pubmed
Heald, A dynamic duo of microtubule modulators. 2000, Pubmed , Xenbase
Helenius, The depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends. 2006, Pubmed
Hezel, LKB1; linking cell structure and tumor suppression. 2008, Pubmed
Holmfeldt, Aneugenic activity of Op18/stathmin is potentiated by the somatic Q18-->e mutation in leukemic cells. 2006, Pubmed
Holmfeldt, Differential functional interplay of TOGp/XMAP215 and the KinI kinesin MCAK during interphase and mitosis. 2004, Pubmed
Holmfeldt, Interphase-specific phosphorylation-mediated regulation of tubulin dimer partitioning in human cells. 2007, Pubmed
Holmfeldt, CaMKIIgamma-mediated inactivation of the Kin I kinesin MCAK is essential for bipolar spindle formation. 2005, Pubmed
Horio, Visualization of the dynamic instability of individual microtubules by dark-field microscopy. , Pubmed
Howard, Microtubule polymerases and depolymerases. 2007, Pubmed , Xenbase
Howell, Decreasing oncoprotein 18/stathmin levels reduces microtubule catastrophes and increases microtubule polymer in vivo. 1999, Pubmed
Howell, Dissociation of the tubulin-sequestering and microtubule catastrophe-promoting activities of oncoprotein 18/stathmin. 1999, Pubmed
Hughes, A microtubule interactome: complexes with roles in cell cycle and mitosis. 2008, Pubmed
Hurov, The Par-1/MARK family of protein kinases: from polarity to metabolism. 2007, Pubmed
Huse, Shouts, whispers and the kiss of death: directional secretion in T cells. 2008, Pubmed
Hyman, Role of GTP hydrolysis in microtubule dynamics: information from a slowly hydrolyzable analogue, GMPCPP. 1992, Pubmed
Jourdain, Stathmin: a tubulin-sequestering protein which forms a ternary T2S complex with two tubulin molecules. 1997, Pubmed
Kerssemakers, Assembly dynamics of microtubules at molecular resolution. 2006, Pubmed
Kim, Cloning and expression of human mitotic centromere-associated kinesin gene. 1997, Pubmed
Kinoshita, XMAP215: a key component of the dynamic microtubule cytoskeleton. 2002, Pubmed , Xenbase
Kinoshita, Global and local control of microtubule destabilization promoted by a catastrophe kinesin MCAK/XKCM1. 2006, Pubmed , Xenbase
Kirschner, Beyond self-assembly: from microtubules to morphogenesis. 1986, Pubmed
Kline-Smith, The microtubule-destabilizing kinesin XKCM1 regulates microtubule dynamic instability in cells. 2002, Pubmed , Xenbase
Kline-Smith, Depletion of centromeric MCAK leads to chromosome congression and segregation defects due to improper kinetochore attachments. 2004, Pubmed , Xenbase
Koppel, Developmental tissue expression and phylogenetic conservation of stathmin, a phosphoprotein associated with cell regulations. 1990, Pubmed
Kremer, Mammalian septins regulate microtubule stability through interaction with the microtubule-binding protein MAP4. 2005, Pubmed
Lan, Aurora B phosphorylates centromeric MCAK and regulates its localization and microtubule depolymerization activity. 2004, Pubmed , Xenbase
Langenickel, KIS protects against adverse vascular remodeling by opposing stathmin-mediated VSMC migration in mice. 2008, Pubmed
Larsson, Control of microtubule dynamics by oncoprotein 18: dissection of the regulatory role of multisite phosphorylation during mitosis. 1997, Pubmed
Larsson, G2/M transition requires multisite phosphorylation of oncoprotein 18 by two distinct protein kinase systems. 1995, Pubmed
Lawler, Microtubule dynamics: if you need a shrink try stathmin/Op18. 1998, Pubmed
Li, Beyond polymer polarity: how the cytoskeleton builds a polarized cell. 2008, Pubmed
Liedtke, Stathmin-deficient mice develop an age-dependent axonopathy of the central and peripheral nervous systems. 2002, Pubmed
Lizcano, LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1. 2004, Pubmed
Mandelkow, Microtubules and microtubule-associated proteins. 1995, Pubmed
Maney, Mitotic centromere-associated kinesin is important for anaphase chromosome segregation. 1998, Pubmed
Manna, Stathmin strongly increases the minus end catastrophe frequency and induces rapid treadmilling of bovine brain microtubules at steady state in vitro. 2006, Pubmed
Marklund, Oncoprotein 18 is a phosphorylation-responsive regulator of microtubule dynamics. 1996, Pubmed
Marklund, Serine 16 of oncoprotein 18 is a major cytosolic target for the Ca2+/calmodulin-dependent kinase-Gr. 1994, Pubmed
Marklund, Serine 25 of oncoprotein 18 is a major cytosolic target for the mitogen-activated protein kinase. 1993, Pubmed
McNally, Katanin-mediated microtubule severing can be regulated by multiple mechanisms. 2002, Pubmed , Xenbase
Melander Gradin, Regulation of microtubule dynamics by Ca2+/calmodulin-dependent kinase IV/Gr-dependent phosphorylation of oncoprotein 18. 1997, Pubmed
Mellman, Coordinated protein sorting, targeting and distribution in polarized cells. 2008, Pubmed
Mitchison, Dynamic instability of microtubule growth. , Pubmed
Mitchison, Some thoughts on the partitioning of tubulin between monomer and polymer under conditions of dynamic instability. 1987, Pubmed
Mori, SCG10-related neuronal growth-associated proteins in neural development, plasticity, degeneration, and aging. 2002, Pubmed
Murphy, Wild-type p53 negatively regulates the expression of a microtubule-associated protein. 1996, Pubmed
Nakamura, Clinicopathological and biological significance of mitotic centromere-associated kinesin overexpression in human gastric cancer. 2007, Pubmed
Ng, Stat3 regulates microtubules by antagonizing the depolymerization activity of stathmin. 2006, Pubmed
Nguyen, Microtubule-associated protein 4 (MAP4) regulates assembly, protomer-polymer partitioning and synthesis of tubulin in cultured cells. 1999, Pubmed
Niethammer, Stathmin-tubulin interaction gradients in motile and mitotic cells. 2004, Pubmed , Xenbase
Nogales, Structural intermediates in microtubule assembly and disassembly: how and why? 2006, Pubmed
Nogales, High-resolution model of the microtubule. 1999, Pubmed
Nogales, Structural insights into microtubule function. 2000, Pubmed
Ohkura, Dis1/TOG universal microtubule adaptors - one MAP for all? 2001, Pubmed
Ookata, MAP4 is the in vivo substrate for CDC2 kinase in HeLa cells: identification of an M-phase specific and a cell cycle-independent phosphorylation site in MAP4. 1997, Pubmed
Ookata, Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics. 1995, Pubmed
Ozon, Drosophila stathmin: a microtubule-destabilizing factor involved in nervous system formation. 2002, Pubmed
Ozon, The stathmin family -- molecular and biological characterization of novel mammalian proteins expressed in the nervous system. 1997, Pubmed , Xenbase
Quarmby, Cellular Samurai: katanin and the severing of microtubules. 2000, Pubmed
Raynaud-Messina, Gamma-tubulin complexes and microtubule organization. 2007, Pubmed
Ringhoff, Stathmin regulates centrosomal nucleation of microtubules and tubulin dimer/polymer partitioning. 2009, Pubmed
Saadat, Helicobacter pylori CagA targets PAR1/MARK kinase to disrupt epithelial cell polarity. 2007, Pubmed
Scholz, Microtubule-associated protein-4 (MAP-4) inhibits microtubule-dependent distribution of mRNA in isolated neonatal cardiocytes. 2006, Pubmed
Schubart, Normal development of mice lacking metablastin (P19), a phosphoprotein implicated in cell cycle regulation. 1996, Pubmed
Sellin, Global regulation of the interphase microtubule system by abundantly expressed Op18/stathmin. 2008, Pubmed
Shiina, Mutations at phosphorylation sites of Xenopus microtubule-associated protein 4 affect its microtubule-binding ability and chromosome movement during mitosis. 1999, Pubmed , Xenbase
Shimo, Involvement of kinesin family member 2C/mitotic centromere-associated kinesin overexpression in mammary carcinogenesis. 2008, Pubmed
Shirasu-Hiza, Identification of XMAP215 as a microtubule-destabilizing factor in Xenopus egg extract by biochemical purification. 2003, Pubmed , Xenbase
Shumyatsky, stathmin, a gene enriched in the amygdala, controls both learned and innate fear. 2005, Pubmed
Singer, Coordinated expression of stathmin family members by far upstream sequence element-binding protein-1 increases motility in non-small cell lung cancer. 2009, Pubmed
Slep, Structural basis of microtubule plus end tracking by XMAP215, CLIP-170, and EB1. 2007, Pubmed
Small, Functional design in the actin cytoskeleton. 1999, Pubmed
Sobel, Stathmin: a relay phosphoprotein for multiple signal transduction? 1991, Pubmed
Spiliotis, Epithelial polarity requires septin coupling of vesicle transport to polyglutamylated microtubules. 2008, Pubmed
Srsen, Serum-dependent phosphorylation of human MAP4 at Ser696 in cultured mammalian cells. 1999, Pubmed
Steinmetz, Op18/stathmin caps a kinked protofilament-like tubulin tetramer. 2000, Pubmed
Stowers, Regulation of the polarization of T cells toward antigen-presenting cells by Ras-related GTPase CDC42. 1995, Pubmed
Tanaka, T helper type 2 differentiation and intracellular trafficking of the interleukin 4 receptor-alpha subunit controlled by the Rac activator Dock2. 2007, Pubmed
Tournebize, Distinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics during mitosis. 1997, Pubmed , Xenbase
Tournebize, Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts. 2000, Pubmed , Xenbase
Vasquez, Phosphorylation by CDK1 regulates XMAP215 function in vitro. 1999, Pubmed , Xenbase
Verma, STAT3-stathmin interactions control microtubule dynamics in migrating T-cells. 2009, Pubmed
Walczak, XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly. 1996, Pubmed , Xenbase
Walczak, Microtubule dynamics and tubulin interacting proteins. 2000, Pubmed , Xenbase
Walczak, Mechanisms of mitotic spindle assembly and function. 2008, Pubmed , Xenbase
Walczak, The microtubule-destabilizing kinesin XKCM1 is required for chromosome positioning during spindle assembly. 2002, Pubmed , Xenbase
Wang, Removal of MAP4 from microtubules in vivo produces no observable phenotype at the cellular level. 1996, Pubmed
Watabe-Uchida, The Rac activator DOCK7 regulates neuronal polarity through local phosphorylation of stathmin/Op18. 2006, Pubmed
Weirich, The septin family of GTPases: architecture and dynamics. 2008, Pubmed
Wiese, Microtubule nucleation: gamma-tubulin and beyond. 2006, Pubmed
Wilde, Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran. 1999, Pubmed , Xenbase
Wittmann, Regulation of microtubule destabilizing activity of Op18/stathmin downstream of Rac1. 2004, Pubmed
Wordeman, Identification and partial characterization of mitotic centromere-associated kinesin, a kinesin-related protein that associates with centromeres during mitosis. 1995, Pubmed
van Breugel, Stu2p, the budding yeast member of the conserved Dis1/XMAP215 family of microtubule-associated proteins is a plus end-binding microtubule destabilizer. 2003, Pubmed , Xenbase