Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2009 Jun 23;10625:10320-5. doi: 10.1073/pnas.0900329106.
Show Gene links
Show Anatomy links
The glutamate receptor subunit delta2 is capable of gating its intrinsic ion channel as revealed by ligand binding domain transplantation.
Schmid SM
,
Kott S
,
Sager C
,
Huelsken T
,
Hollmann M
.
???displayArticle.abstract???
The family of ionotropic glutamate receptors includes 2 subunits, delta1 and delta2, the physiological relevance of which remains poorly understood. Both are nonfunctional in heterologous expression systems, although the isolated, crystallized ligand binding domain (LBD) of delta2 is capable of binding D-serine. To investigate these seemingly contradictory observations we tested whether delta receptors can be ligand gated at all. We used a strategy that replaced the native LBD of delta2 by a proven glutamate-binding LBD. Test transplantations between alpha-amino-3-hydroxy-5-methylisoxazole propionate (AMPA) and kainate receptors (GluR1 and GluR6, respectively) showed that this approach can produce functional chimeras even if only one part of the bipartite LBD is swapped. Upon outfitting delta2 with the LBD of GluR6, the chimera formed glutamate-gated ion channels with low Ca(2+) permeability and unique rectification properties. Ligand-induced conformational changes can thus gate delta2, suggesting that the LBD of this receptor works fundamentally differently from that of other ionotropic glutamate receptors.
Armstrong,
Mechanisms for activation and antagonism of an AMPA-sensitive glutamate receptor: crystal structures of the GluR2 ligand binding core.
2000, Pubmed
Armstrong,
Mechanisms for activation and antagonism of an AMPA-sensitive glutamate receptor: crystal structures of the GluR2 ligand binding core.
2000,
Pubmed
Armstrong,
Measurement of conformational changes accompanying desensitization in an ionotropic glutamate receptor.
2006,
Pubmed
,
Xenbase
Balannik,
Molecular mechanism of AMPA receptor noncompetitive antagonism.
2005,
Pubmed
,
Xenbase
Egebjerg,
Ca2+ permeability of unedited and edited versions of the kainate selective glutamate receptor GluR6.
1993,
Pubmed
,
Xenbase
Everts,
N-Glycosylation is not a prerequisite for glutamate receptor function but Is essential for lectin modulation.
1997,
Pubmed
,
Xenbase
Hansen,
Modulation of the dimer interface at ionotropic glutamate-like receptor delta2 by D-serine and extracellular calcium.
2009,
Pubmed
,
Xenbase
Hirai,
Rescue of abnormal phenotypes of the delta2 glutamate receptor-null mice by mutant delta2 transgenes.
2005,
Pubmed
Hirano,
Involvement of the glutamate receptor delta 2 subunit in the long-term depression of glutamate responsiveness in cultured rat Purkinje cells.
1994,
Pubmed
Hirano,
Suppression of LTD in cultured Purkinje cells deficient in the glutamate receptor delta 2 subunit.
1995,
Pubmed
Hollmann,
Cloned glutamate receptors.
1994,
Pubmed
Jeromin,
Suppression of the glutamate receptor delta 2 subunit produces a specific impairment in cerebellar long-term depression.
1996,
Pubmed
Jin,
Mechanism of positive allosteric modulators acting on AMPA receptors.
2005,
Pubmed
Kakegawa,
The delta2 'ionotropic' glutamate receptor functions as a non-ionotropic receptor to control cerebellar synaptic plasticity.
2007,
Pubmed
Kakegawa,
Ca2+ permeability of the channel pore is not essential for the delta2 glutamate receptor to regulate synaptic plasticity and motor coordination.
2007,
Pubmed
Kashiwabuchi,
Impairment of motor coordination, Purkinje cell synapse formation, and cerebellar long-term depression in GluR delta 2 mutant mice.
1995,
Pubmed
Kohda,
Mutation of a glutamate receptor motif reveals its role in gating and delta2 receptor channel properties.
2000,
Pubmed
Kuroyanagi,
Postsynaptic glutamate receptor delta family contributes to presynaptic terminal differentiation and establishment of synaptic transmission.
2009,
Pubmed
Leuschner,
Subtype-specific assembly of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits is mediated by their n-terminal domains.
1999,
Pubmed
Milstein,
Regulation of AMPA receptor gating and pharmacology by TARP auxiliary subunits.
2008,
Pubmed
Morimoto-Tomita,
Autoinactivation of neuronal AMPA receptors via glutamate-regulated TARP interaction.
2009,
Pubmed
,
Xenbase
Naur,
Ionotropic glutamate-like receptor delta2 binds D-serine and glycine.
2007,
Pubmed
Panicker,
Synaptic AMPA receptor subunit trafficking is independent of the C terminus in the GluR2-lacking mouse.
2008,
Pubmed
Schmid,
A domain linking the AMPA receptor agonist binding site to the ion pore controls gating and causes lurcher properties when mutated.
2007,
Pubmed
,
Xenbase
Schmid,
To gate or not to gate: are the delta subunits in the glutamate receptor family functional ion channels?
2008,
Pubmed
Schwenk,
Functional proteomics identify cornichon proteins as auxiliary subunits of AMPA receptors.
2009,
Pubmed
,
Xenbase
Sommer,
A glutamate receptor channel with high affinity for domoate and kainate.
1992,
Pubmed
Soto,
P2X4: an ATP-activated ionotropic receptor cloned from rat brain.
1996,
Pubmed
,
Xenbase
Stern-Bach,
A point mutation in the glutamate binding site blocks desensitization of AMPA receptors.
1998,
Pubmed
Sun,
Mechanism of glutamate receptor desensitization.
2002,
Pubmed
Swanson,
Identification of amino acid residues that control functional behavior in GluR5 and GluR6 kainate receptors.
1997,
Pubmed
Uemura,
The amino-terminal domain of glutamate receptor delta2 triggers presynaptic differentiation.
2008,
Pubmed
Villmann,
Investigation by ion channel domain transplantation of rat glutamate receptor subunits, orphan receptors and a putative NMDA receptor subunit.
1999,
Pubmed
,
Xenbase
Weston,
Interdomain interactions in AMPA and kainate receptors regulate affinity for glutamate.
2006,
Pubmed
Williams,
Pharmacology of delta2 glutamate receptors: effects of pentamidine and protons.
2003,
Pubmed
,
Xenbase
Wollmuth,
The Lurcher mutation identifies delta 2 as an AMPA/kainate receptor-like channel that is potentiated by Ca(2+).
2000,
Pubmed
Yuzaki,
Transgenic rescue for characterizing orphan receptors: a review of delta2 glutamate receptor.
2005,
Pubmed
Zhang,
A transmembrane accessory subunit that modulates kainate-type glutamate receptors.
2009,
Pubmed
Zuo,
Neurodegeneration in Lurcher mice caused by mutation in delta2 glutamate receptor gene.
1997,
Pubmed
,
Xenbase
