Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-40202
PLoS Biol August 1, 2009; 7 (8): e1000174.

Generation of functional eyes from pluripotent cells.

Viczian AS , Solessio EC , Lyou Y , Zuber ME .


Abstract
Pluripotent cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells are the starting point from which to generate organ specific cell types. For example, converting pluripotent cells to retinal cells could provide an opportunity to treat retinal injuries and degenerations. In this study, we used an in vivo strategy to determine if functional retinas could be generated from a defined population of pluripotent Xenopus laevis cells. Animal pole cells isolated from blastula stage embryos are pluripotent. Untreated, these cells formed only epidermis, when transplanted to either the flank or eye field. In contrast, misexpression of seven transcription factors induced the formation of retinal cell types. Induced retinal cells were committed to a retinal lineage as they formed eyes when transplanted to the flanks of developing embryos. When the endogenous eye field was replaced with induced retinal cells, they formed eyes that were molecularly, anatomically, and electrophysiologically similar to normal eyes. Importantly, induced eyes could guide a vision-based behavior. These results suggest the fate of pluripotent cells may be purposely altered to generate multipotent retinal progenitor cells, which differentiate into functional retinal cell classes and form a neural circuitry sufficient for vision.

PubMed ID: 19688031
PMC ID: PMC2716519
Article link: PLoS Biol
Grant support: [+]
Genes referenced: calb1 calb2 erg isl1 nog pax6 pc.1 rax rbpms rbpms2 rpe six3 tfap2a th
Antibodies: Calb1 Ab1 Calb2 Ab1 GABA Ab1 GFP Ab4 GFP Ab5 Isl1/2 Ab1 Muller Glia Ab1 Photoreceptors Ab1 Th Ab1

GEO Series:
   GSE9173: NCBI
   GSE9175: NCBI


Article Images: [+] show captions
References [+] :
Agathocleous, A general role of hedgehog in the regulation of proliferation. 2007, Pubmed


Xenbase: The Xenopus Model Organism Knowledgebase.
Version: 4.14.0
Major funding for Xenbase is provided by grant P41 HD064556