Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Ren J
,
Liu Z
,
Gao X
,
Jin C
,
Ye M
,
Zou H
,
Wen L
,
Zhang Z
,
Xue Y
,
Yao X
.
???displayArticle.abstract???
During cell division/mitosis, a specific subset of proteins is spatially and temporally assembled into protein super complexes in three distinct regions, i.e. centrosome/spindle pole, kinetochore/centromere and midbody/cleavage furrow/phragmoplast/bud neck, and modulates cell division process faithfully. Although many experimental efforts have been carried out to investigate the characteristics of these proteins, no integrated database was available. Here, we present the MiCroKit database (http://microkit.biocuckoo.org) of proteins that localize in midbody, centrosome and/or kinetochore. We collected into the MiCroKit database experimentally verified microkit proteins from the scientific literature that have unambiguous supportive evidence for subcellular localization under fluorescent microscope. The current version of MiCroKit 3.0 provides detailed information for 1489 microkit proteins from seven model organisms, including Saccharomyces cerevisiae, Schizasaccharomyces pombe, Caenorhabditis elegans, Drosophila melanogaster, Xenopus laevis, Mus musculus and Homo sapiens. Moreover, the orthologous information was provided for these microkit proteins, and could be a useful resource for further experimental identification. The online service of MiCroKit database was implemented in PHP + MySQL + JavaScript, while the local packages were developed in JAVA 1.5 (J2SE 5.0).
Figure 1. The search option of MiCroKit 3.0 database. (A) Users could simply input ‘survivin’ for querying. (B) The results will be shown in a tabular format. Users could click on the MiCroKit ID (MCK-HS-00222) to visualize the detailed information. (C) The detailed information of human Survivin. The orthologous information was pre-calculated and manually checked.
Figure 2. Five advance options in MiCroKit 3.0. (A) Advance search allows users to input up to three terms for querying; (B) browse and BLAST search; (C) orthologous group browse and Pairwise orthologous browse; (D) the default example of pairwise orthologous browse. The orthologous information (identity ≥ 20%) between M. musculus and H. sapiens will be shown in details. The proteins collected in the MiCroKit database are marked in bold.
Figure 3. The statistics of localization distributions of microkit proteins from seven organisms, separately.
Andersen,
Proteomic characterization of the human centrosome by protein correlation profiling.
2003, Pubmed
Andersen,
Proteomic characterization of the human centrosome by protein correlation profiling.
2003,
Pubmed
Badano,
The centrosome in human genetic disease.
2005,
Pubmed
Bakhoum,
Genome stability is ensured by temporal control of kinetochore-microtubule dynamics.
2009,
Pubmed
Basto,
Centrosome amplification can initiate tumorigenesis in flies.
2008,
Pubmed
Bjellqvist,
The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences.
1993,
Pubmed
Bjellqvist,
Reference points for comparisons of two-dimensional maps of proteins from different human cell types defined in a pH scale where isoelectric points correlate with polypeptide compositions.
1994,
Pubmed
Cheeseman,
Molecular architecture of the kinetochore-microtubule interface.
2008,
Pubmed
Doxsey,
Centrosomes in cellular regulation.
2005,
Pubmed
Fukagawa,
Assembly of kinetochores in vertebrate cells.
2004,
Pubmed
Ganem,
A mechanism linking extra centrosomes to chromosomal instability.
2009,
Pubmed
Guertin,
Cytokinesis in eukaryotes.
2002,
Pubmed
Jaspersen,
The budding yeast spindle pole body: structure, duplication, and function.
2004,
Pubmed
Johnson,
NCBI BLAST: a better web interface.
2008,
Pubmed
Kuo,
Survivin and p53 modulate quercetin-induced cell growth inhibition and apoptosis in human lung carcinoma cells.
2004,
Pubmed
Lee,
Midbody targeting of the ESCRT machinery by a noncanonical coiled coil in CEP55.
2008,
Pubmed
Li,
Pleiotropic cell-division defects and apoptosis induced by interference with survivin function.
1999,
Pubmed
Nogales-Cadenas,
CentrosomeDB: a human centrosomal proteins database.
2009,
Pubmed
O'Brien,
Inparanoid: a comprehensive database of eukaryotic orthologs.
2005,
Pubmed
Otegui,
Midbodies and phragmoplasts: analogous structures involved in cytokinesis.
2005,
Pubmed
Pelletier,
Centrosomes: keeping tumors in check.
2008,
Pubmed
Pohl,
Midbody ring disposal by autophagy is a post-abscission event of cytokinesis.
2009,
Pubmed
Pohl,
Final stages of cytokinesis and midbody ring formation are controlled by BRUCE.
2008,
Pubmed
Remm,
Automatic clustering of orthologs and in-paralogs from pairwise species comparisons.
2001,
Pubmed
Sakuno,
Kinetochore geometry defined by cohesion within the centromere.
2009,
Pubmed
Skop,
Dissection of the mammalian midbody proteome reveals conserved cytokinesis mechanisms.
2004,
Pubmed
Tanaka,
Kinetochore-microtubule interactions: the means to the end.
2008,
Pubmed
Tatusov,
A genomic perspective on protein families.
1997,
Pubmed
UniProt Consortium,
The Universal Protein Resource (UniProt) 2009.
2009,
Pubmed
Wan,
Protein architecture of the human kinetochore microtubule attachment site.
2009,
Pubmed
Westermann,
Structures and functions of yeast kinetochore complexes.
2007,
Pubmed
Yang,
Extra centrosomes and/or chromosomes prolong mitosis in human cells.
2008,
Pubmed
Yang,
Phosphorylation of HsMis13 by Aurora B kinase is essential for assembly of functional kinetochore.
2008,
Pubmed
Yang,
Cell division and cell survival in the absence of survivin.
2004,
Pubmed
Yu,
The plant kinetochore.
2000,
Pubmed
