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Historically, much of our understanding of actin filaments, microtubules and intermediate filaments has come from the study of fixed cells and tissues. But the cytoskeleton is inherently dynamic, and so developing the means to image it in living cells has proved crucial. Advances in confocal microscopy and fluorescent protein technologies have allowed us to dynamically image the cytoskeleton at high resolution and so learn much more about its cellular functions. However, most of this work has been performed in cultured cells, and a critical next step is to understand how the cytoskeleton functions in the context of an intact organism. We, and others, have developed methods to image the cytoskeleton in living vertebrate embryos. Here, we describe an approach to image the cytoskeleton in embryos of the frog, Xenopus laevis, using mRNA to express fluorescently tagged cytoskeletal probes and confocal microscopy to visualize their dynamic behavior.
Bement,
A microtubule-dependent zone of active RhoA during cleavage plane specification.
2005, Pubmed,
Xenbase
Bement,
A microtubule-dependent zone of active RhoA during cleavage plane specification.
2005,
Pubmed
,
Xenbase
Benink,
Concentric zones of active RhoA and Cdc42 around single cell wounds.
2005,
Pubmed
,
Xenbase
Burkel,
Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin.
2007,
Pubmed
,
Xenbase
Campbell,
A monomeric red fluorescent protein.
2002,
Pubmed
Chalfie,
Green fluorescent protein as a marker for gene expression.
1994,
Pubmed
Charras,
Reassembly of contractile actin cortex in cell blebs.
2006,
Pubmed
Faire,
E-MAP-115 (ensconsin) associates dynamically with microtubules in vivo and is not a physiological modulator of microtubule dynamics.
1999,
Pubmed
Heasman,
Morpholino oligos: making sense of antisense?
2002,
Pubmed
,
Xenbase
Heim,
Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer.
1996,
Pubmed
Kieserman,
Developmental regulation of central spindle assembly and cytokinesis during vertebrate embryogenesis.
2008,
Pubmed
,
Xenbase
Kim,
E-cadherin-mediated cell-cell attachment activates Cdc42.
2000,
Pubmed
Ma,
Cdc42 activation couples spindle positioning to first polar body formation in oocyte maturation.
2006,
Pubmed
,
Xenbase
Matz,
Fluorescent proteins from nonbioluminescent Anthozoa species.
1999,
Pubmed
,
Xenbase
Megason,
Digitizing life at the level of the cell: high-performance laser-scanning microscopy and image analysis for in toto imaging of development.
2003,
Pubmed
Mikhailov,
Relationship between microtubule dynamics and lamellipodium formation revealed by direct imaging of microtubules in cells treated with nocodazole or taxol.
1998,
Pubmed
Pertz,
Designing biosensors for Rho family proteins--deciphering the dynamics of Rho family GTPase activation in living cells.
2004,
Pubmed
Prasher,
Primary structure of the Aequorea victoria green-fluorescent protein.
1992,
Pubmed
Shaner,
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
2004,
Pubmed
Sokac,
Cdc42-dependent actin polymerization during compensatory endocytosis in Xenopus eggs.
2003,
Pubmed
,
Xenbase
Woolner,
Myosin-10 and actin filaments are essential for mitotic spindle function.
2008,
Pubmed
,
Xenbase
Yonemura,
Rho localization in cells and tissues.
2004,
Pubmed
,
Xenbase
