Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Methods Mol Biol
2009 Jan 01;582:221-31. doi: 10.1007/978-1-60761-340-4_17.
Show Gene links
Show Anatomy links
Analysis of SUMOylation of topoisomerase IIalpha with Xenopus egg extracts.
Azuma Y
.
???displayArticle.abstract???
Posttranslational protein modification by the Small Ubiquitin-like MOdifiers (SUMO) is involved in many cellular functions including organization of nuclear structures and chromatin, transcriptional regulation, and nucleo-cytoplasmic transport. Both genetic and biochemical studies indicate that the SUMO modification pathway plays an important role in proper cell cycle control, especially in the normal progression of mitosis. DNA topoisomerase II has been shown to be modified by SUMO in budding yeast as well as in vertebrates. We have shown by biochemical analysis using the Xenopus egg extract (XEE) cell-free assay system that DNA topoisomerase IIalpha (Topo IIalpha) is modified by SUMO-2/3 on mitotic chromosomes in the early stages of mitosis. Inhibition of mitotic SUMOylation in the XEE assay system causes aberrant sister chromatid separation in anaphase and alters Topo IIalpha association with chromosomes.
Adachi,
Chromosome assembly in vitro: topoisomerase II is required for condensation.
1991, Pubmed,
Xenbase
Adachi,
Chromosome assembly in vitro: topoisomerase II is required for condensation.
1991,
Pubmed
,
Xenbase
Agostinho,
Conjugation of human topoisomerase 2 alpha with small ubiquitin-like modifiers 2/3 in response to topoisomerase inhibitors: cell cycle stage and chromosome domain specificity.
2008,
Pubmed
,
Xenbase
Azuma,
SUMO-2/3 regulates topoisomerase II in mitosis.
2003,
Pubmed
,
Xenbase
Bachant,
The SUMO-1 isopeptidase Smt4 is linked to centromeric cohesion through SUMO-1 modification of DNA topoisomerase II.
2002,
Pubmed
Chen,
Characterization of spindle assembly checkpoint in Xenopus egg extracts.
1997,
Pubmed
,
Xenbase
Cuvier,
A role of topoisomerase II in linking DNA replication to chromosome condensation.
2003,
Pubmed
,
Xenbase
Dasso,
Emerging roles of the SUMO pathway in mitosis.
2008,
Pubmed
Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase
Hay,
SUMO: a history of modification.
2005,
Pubmed
Hay,
SUMO-specific proteases: a twist in the tail.
2007,
Pubmed
Hirano,
Topoisomerase II does not play a scaffolding role in the organization of mitotic chromosomes assembled in Xenopus egg extracts.
1993,
Pubmed
,
Xenbase
Holm,
DNA topoisomerase II is required at the time of mitosis in yeast.
1985,
Pubmed
Johnson,
Protein modification by SUMO.
2004,
Pubmed
Mao,
SUMO-1 conjugation to human DNA topoisomerase II isozymes.
2000,
Pubmed
Mukhopadhyay,
Modification in reverse: the SUMO proteases.
2007,
Pubmed
Shamu,
Sister chromatid separation in frog egg extracts requires DNA topoisomerase II activity during anaphase.
1992,
Pubmed
,
Xenbase
Takahashi,
SIZ1/SIZ2 control of chromosome transmission fidelity is mediated by the sumoylation of topoisomerase II.
2006,
Pubmed
Uemura,
Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization.
1984,
Pubmed
Yanagida,
Basic mechanism of eukaryotic chromosome segregation.
2005,
Pubmed
