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XB-ART-40422
Dev Dyn October 1, 2009; 238 (10): 2505-10.

Xenopus insm1 is essential for gastrointestinal and pancreatic endocrine cell development.

Horb LD , Jarkji ZH , Horb ME .


Abstract
In mammals, it has been well established that gastrointestinal and pancreatic endocrine cells are specified by a cascade of different transcription factors, but whether these same pathways (or linear relationships) operate in Xenopus is currently unknown. We recently identified the endocrine-specific zinc finger transcription factor insulinoma associated protein 1 (insm1) as a dorsal-enriched gene. We found that insm1 is expressed in the dorsal pancreas as early as NF28, making it one of the earliest markers to be localized to the dorsal pancreas. Through morpholino-mediated knockdown, we demonstrate that insm1 is essential for proper specification of both gastrointestinal and pancreatic endocrine cells. In addition, we place insm1 downstream of ngn3 and upstream of pax6 and neuroD in the endocrine cell transcription factor cascade. These are the first results showing that the endodermal endocrine cell development in Xenopus uses the same transcriptional cascade as in mammals.

PubMed ID: 19705447
PMC ID: PMC2921606
Article link: Dev Dyn
Grant support: [+]
Genes referenced: gcg hhex ins insm1 neurod1 neurog3 onecut1 pax6 pdia2 pdx1 ptf1a sfrp5 sst.1
Morpholinos: insm1 MO1 insm1 MO2


Article Images: [+] show captions
References:
Afelik, 2006, Pubmed, Xenbase [+]


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