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XB-ART-40675
Dev Biol. September 1, 2009; 333 (1): 26-36.

Dazap2 is required for FGF-mediated posterior neural patterning, independent of Wnt and Cdx function.

Roche DD , Liu KJ , Harland RM , Monsoro-Burq AH .


Abstract
The organization of the embryonic neural plate requires coordination of multiple signal transduction pathways, including fibroblast growth factors (FGFs), bone morphogenetic proteins (BMPs), and WNTs. Many studies have suggested that a critical component of this process is the patterning of posterior neural tissues by an FGF-caudal signaling cascade. Here, we have identified a novel player, Dazap2, and show that it is required in vivo for posterior neural fate. Loss of Dazap2 in embryos resulted in diminished expression of hoxb9 with a concurrent increase in the anterior marker otx2. Furthermore, we found that Dazap2 is required for FGF dependent posterior patterning; surprisingly, this is independent of Cdx activity. Furthermore, in contrast to FGF activity, Dazap2 induction of hoxb9 is not blocked by loss of canonical Wnt signaling. Functionally, we found that increasing Dazap2 levels alters neural patterning and induces posterior neural markers. This activity overcomes the anteriorizing effects of noggin, and is downstream of FGF receptor activation. Our results strongly suggest that Dazap2 is a novel and essential branch of FGF-induced neural patterning.

PubMed ID: 19555680
PMC ID: PMC3769164
Article link: Dev Biol.
Grant support: GM42341 NIGMS NIH HHSBiotechnology and Biological Sciences Research CouncilWellcome Trust , BB/E013872/1 Biotechnology and Biological Sciences Research Council , R01 GM042341 NIGMS NIH HHS , GM42341 NIGMS NIH HHS , R01 GM042341 NIGMS NIH HHSBiotechnology and Biological Sciences Research CouncilWellcome Trust , BB/E013872/1 Biotechnology and Biological Sciences Research Council , GM42341 NIGMS NIH HHS , R01 GM042341 NIGMS NIH HHSBiotechnology and Biological Sciences Research Council , BB_BB/E013872/1 Biotechnology and Biological Sciences Research CouncilWellcome Trust , GM42341 NIGMS NIH HHS , R01 GM042341 NIGMS NIH HHSBiotechnology and Biological Sciences Research CouncilWellcome Trust , BB/E013872/1 Biotechnology and Biological Sciences Research Council

Genes referenced: cdx1 cdx4 dazap1 dazap2 egr2 en2 f8a1 fgf8 hoxb9 hoxd1 hspa9 myod1 nog nrp1 otx2 pax6 ptgds sox11 sox2 tdrd6

Morpholinos referenced: dazap2 MO1 dazap2 MO2

References:
Blumberg, 1997, Pubmed, Xenbase[+]


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