XB-ART-40744
Blood
January 28, 2010;
115
(4):
906-9.
Liprin (beta)1 is highly expressed in lymphatic vasculature and is important for lymphatic vessel integrity.
Norrmén C
,
Vandevelde W
,
Ny A
,
Saharinen P
,
Gentile M
,
Haraldsen G
,
Puolakkainen P
,
Lukanidin E
,
Dewerchin M
,
Alitalo K
,
Petrova TV
.
Abstract
The lymphatic
vasculature is important for the regulation of
tissue fluid homeostasis, immune response, and lipid absorption, and the development of in vitro models should allow for a better understanding of the mechanisms regulating lymphatic vascular growth, repair, and function. Here we report isolation and characterization of lymphatic endothelial cells from human
intestine and show that intestinal lymphatic endothelial cells have a related but distinct gene expression profile from human dermal lymphatic endothelial cells. Furthermore, we identify liprin beta1, a member of the family of
LAR transmembrane tyrosine phosphatase-interacting proteins, as highly expressed in intestinal lymphatic endothelial cells in vitro and lymphatic
vasculature in vivo, and show that it plays an important role in the maintenance of
lymphatic vessel integrity in Xenopus tadpoles.
PubMed ID:
19965622
PMC ID:
PMC2815506
Article link:
Blood
Grant support:
[+]
Species referenced:
Xenopus laevis
Genes referenced:
ppfibp1
ptprf
Morpholinos:
ppfibp1 MO1
ppfibp1 MO2
References [+] :
Garrafa,
Isolation, purification, and heterogeneity of human lymphatic endothelial cells from different tissues.
2006,
Pubmed
Garrafa,
Isolation, purification, and heterogeneity of human lymphatic endothelial cells from different tissues.
2006,
Pubmed
Haraldsen,
Isolation and longterm culture of human intestinal microvascular endothelial cells.
1995,
Pubmed
Hirakawa,
Identification of vascular lineage-specific genes by transcriptional profiling of isolated blood vascular and lymphatic endothelial cells.
2003,
Pubmed
Jahnsen,
Expression of functional VCAM-1 by cultured nasal polyp-derived microvascular endothelium.
1997,
Pubmed
Karkkainen,
Vascular endothelial growth factor C is required for sprouting of the first lymphatic vessels from embryonic veins.
2003,
Pubmed
Kaufmann,
Drosophila liprin-alpha and the receptor phosphatase Dlar control synapse morphogenesis.
2002,
Pubmed
Ko,
Interaction between liprin-alpha and GIT1 is required for AMPA receptor targeting.
2003,
Pubmed
Kriajevska,
Liprin beta 1, a member of the family of LAR transmembrane tyrosine phosphatase-interacting proteins, is a new target for the metastasis-associated protein S100A4 (Mts1).
2002,
Pubmed
Kriehuber,
Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages.
2001,
Pubmed
Miller,
Direct observation demonstrates that Liprin-alpha is required for trafficking of synaptic vesicles.
2005,
Pubmed
Mäkinen,
Isolated lymphatic endothelial cells transduce growth, survival and migratory signals via the VEGF-C/D receptor VEGFR-3.
2001,
Pubmed
Norrmén,
FOXC2 controls formation and maturation of lymphatic collecting vessels through cooperation with NFATc1.
2009,
Pubmed
Ny,
A genetic Xenopus laevis tadpole model to study lymphangiogenesis.
2005,
Pubmed
,
Xenbase
Petrova,
Defective valves and abnormal mural cell recruitment underlie lymphatic vascular failure in lymphedema distichiasis.
2004,
Pubmed
Podgrabinska,
Molecular characterization of lymphatic endothelial cells.
2002,
Pubmed
Serra-Pagès,
The LAR transmembrane protein tyrosine phosphatase and a coiled-coil LAR-interacting protein co-localize at focal adhesions.
1995,
Pubmed
Serra-Pagès,
Liprins, a family of LAR transmembrane protein-tyrosine phosphatase-interacting proteins.
1998,
Pubmed
Wyszynski,
Interaction between GRIP and liprin-alpha/SYD2 is required for AMPA receptor targeting.
2002,
Pubmed