Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Membr Biol
2010 Feb 01;2331-3:35-41. doi: 10.1007/s00232-009-9222-0.
Show Gene links
Show Anatomy links
Regulation of the Ca(2+) channel TRPV6 by the kinases SGK1, PKB/Akt, and PIKfyve.
Sopjani M
,
Kunert A
,
Czarkowski K
,
Klaus F
,
Laufer J
,
Föller M
,
Lang F
.
???displayArticle.abstract???
The serum- and glucocorticoid-inducible kinase SGK1 and the protein kinase PKB/Akt presumably phosphorylate and, by this means, activate the mammalian phosphatidylinositol-3-phosphate-5-kinase PIKfyve (PIP5K3), which has in turn been shown to regulate transporters and channels. SGK1-regulated channels include the Ca(2+) channel TRPV6, which is expressed in a variety of epithelial and nonepithelial cells including tumor cells. SGK1 and protein kinase B PKB/Akt foster tumor growth. The present study thus explored whether TRPV6 is regulated by PIKfyve. TRPV6 was expressed in Xenopus laevis oocytes with or without additional coexpression of constitutively active (S422D)SGK1, constitutively active (T308D,S473D)PKB, wild-type PIKfyve, and (S318A)PIKfyve lacking the SGK1 phosphorylation site. TRPV6 activity was determined from the current (I(Ca)) resulting from TRPV6-induced Ca(2+) entry and subsequent activation of Ca(2+)-sensitive endogenous Cl(-) channels. TRPV6 protein abundance in the cell membrane was determined utilizing immunohistochemistry and Western blotting. In TRPV6-expressing oocytes I(H) was increased by coexpression of (S422D)SGK1 and by (T308D,S473D)PKB. Coexpression of wild-type PIKfyve further increased I(H) in TRPV6 + (S422D)SGK1-expressing oocytes but did not significantly modify I(Ca) in oocytes expressing TRPV6 alone. (S318A)PIKfyve failed to significantly modify I(Ca) in the presence and absence of (S422D)SGK1. (S422D)SGK1 increased the TRPV6 protein abundance in the cell membrane, an effect augmented by additional expression of wild-type PIKfyve. We conclude that PIKfyve participates in the regulation of TRPV6.
Berwick,
Protein kinase B phosphorylation of PIKfyve regulates the trafficking of GLUT4 vesicles.
2004, Pubmed
Berwick,
Protein kinase B phosphorylation of PIKfyve regulates the trafficking of GLUT4 vesicles.
2004,
Pubmed
Boehmer,
Modulation of the voltage-gated potassium channel Kv1.5 by the SGK1 protein kinase involves inhibition of channel ubiquitination.
2008,
Pubmed
,
Xenbase
Boehmer,
The peptide transporter PEPT2 is targeted by the protein kinase SGK1 and the scaffold protein NHERF2.
2008,
Pubmed
,
Xenbase
Bolanz,
The role of TRPV6 in breast carcinogenesis.
2008,
Pubmed
Böhmer,
Regulation of the epithelial calcium channel TRPV6 by the serum and glucocorticoid-inducible kinase isoforms SGK1 and SGK3.
2007,
Pubmed
,
Xenbase
Embark,
Regulation of CLC-Ka/barttin by the ubiquitin ligase Nedd4-2 and the serum- and glucocorticoid-dependent kinases.
2004,
Pubmed
,
Xenbase
Fukushima,
Short-chain fatty acids induce intestinal transient receptor potential vanilloid type 6 expression in rats and Caco-2 cells.
2009,
Pubmed
Grahammer,
Intestinal function of gene-targeted mice lacking serum- and glucocorticoid-inducible kinase 1.
2006,
Pubmed
Heine,
1,25-dihydroxyvitamin D(3) promotes IL-10 production in human B cells.
2008,
Pubmed
Ikonomov,
Mammalian cell morphology and endocytic membrane homeostasis require enzymatically active phosphoinositide 5-kinase PIKfyve.
2001,
Pubmed
Ikonomov,
Localized PtdIns 3,5-P2 synthesis to regulate early endosome dynamics and fusion.
2006,
Pubmed
Ikonomov,
Functional dissection of lipid and protein kinase signals of PIKfyve reveals the role of PtdIns 3,5-P2 production for endomembrane integrity.
2002,
Pubmed
Ikonomov,
PIKfyve controls fluid phase endocytosis but not recycling/degradation of endocytosed receptors or sorting of procathepsin D by regulating multivesicular body morphogenesis.
2003,
Pubmed
Klaus,
Up-regulation of hypertonicity-activated myo-inositol transporter SMIT1 by the cell volume-sensitive protein kinase SGK1.
2008,
Pubmed
,
Xenbase
Klaus,
PIKfyve-dependent regulation of the Cl- channel ClC-2.
2009,
Pubmed
,
Xenbase
Lallet-Daher,
Intermediate-conductance Ca2+-activated K+ channels (IKCa1) regulate human prostate cancer cell proliferation through a close control of calcium entry.
2009,
Pubmed
Lang,
(Patho)physiological significance of the serum- and glucocorticoid-inducible kinase isoforms.
2006,
Pubmed
Lehen'kyi,
TRPV6 channel controls prostate cancer cell proliferation via Ca(2+)/NFAT-dependent pathways.
2007,
Pubmed
Prevarskaya,
TRP channels in cancer.
2007,
Pubmed
Rexhepaj,
Stimulation of electrogenic intestinal dipeptide transport by the glucocorticoid dexamethasone.
2009,
Pubmed
,
Xenbase
Rotte,
APC sensitive gastric acid secretion.
2009,
Pubmed
Rotte,
Pioglitazone induced gastric acid secretion.
2009,
Pubmed
Rusten,
Fab1 phosphatidylinositol 3-phosphate 5-kinase controls trafficking but not silencing of endocytosed receptors.
2006,
Pubmed
Rutherford,
The mammalian phosphatidylinositol 3-phosphate 5-kinase (PIKfyve) regulates endosome-to-TGN retrograde transport.
2006,
Pubmed
Sandu,
Role of the serum and glucocorticoid inducible kinase SGK1 in glucocorticoid stimulation of gastric acid secretion.
2007,
Pubmed
Sato,
Regulation of human cystic fibrosis transmembrane conductance regulator (CFTR) by serum- and glucocorticoid-inducible kinase (SGK1).
2007,
Pubmed
,
Xenbase
Sbrissa,
PIKfyve, a mammalian ortholog of yeast Fab1p lipid kinase, synthesizes 5-phosphoinositides. Effect of insulin.
1999,
Pubmed
Sbrissa,
Role for a novel signaling intermediate, phosphatidylinositol 5-phosphate, in insulin-regulated F-actin stress fiber breakdown and GLUT4 translocation.
2004,
Pubmed
Sbrissa,
Phosphatidylinositol 5-phosphate biosynthesis is linked to PIKfyve and is involved in osmotic response pathway in mammalian cells.
2002,
Pubmed
Seebohm,
Regulation of endocytic recycling of KCNQ1/KCNE1 potassium channels.
2007,
Pubmed
,
Xenbase
Semenova,
Endogenous expression of TRPV5 and TRPV6 calcium channels in human leukemia K562 cells.
2009,
Pubmed
Shaw,
The role of SGK and CFTR in acute adaptation to seawater in Fundulus heteroclitus.
2008,
Pubmed
,
Xenbase
Shojaiefard,
Regulation of the Na(+), glucose cotransporter by PIKfyve and the serum and glucocorticoid inducible kinase SGK1.
2007,
Pubmed
,
Xenbase
Strutz-Seebohm,
Comparison of potent Kv1.5 potassium channel inhibitors reveals the molecular basis for blocking kinetics and binding mode.
2007,
Pubmed
Strutz-Seebohm,
PIKfyve in the SGK1 mediated regulation of the creatine transporter SLC6A8.
2007,
Pubmed
,
Xenbase
Strutz-Seebohm,
Regulation of GluR1 abundance in murine hippocampal neurones by serum- and glucocorticoid-inducible kinase 3.
2005,
Pubmed
,
Xenbase
Stumpf,
The human TRPV6 channel protein is associated with cyclophilin B in human placenta.
2008,
Pubmed
,
Xenbase
Takumida,
Age-dependent changes in the expression of klotho protein, TRPV5 and TRPV6 in mouse inner ear.
2009,
Pubmed
Thyagarajan,
Phospholipase C-mediated regulation of transient receptor potential vanilloid 6 channels: implications in active intestinal Ca2+ transport.
2009,
Pubmed
Thyagarajan,
Hydrolysis of phosphatidylinositol 4,5-bisphosphate mediates calcium-induced inactivation of TRPV6 channels.
2008,
Pubmed
Watson,
Bridging the GAP between insulin signaling and GLUT4 translocation.
2006,
Pubmed
Welsh,
Role of protein kinase B in insulin-regulated glucose uptake.
2005,
Pubmed
Wulff,
Impaired renal Na(+) retention in the sgk1-knockout mouse.
2002,
Pubmed
,
Xenbase
van de Graaf,
Regulation of TRPV5 and TRPV6 by associated proteins.
2006,
Pubmed
