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XB-ART-40889
Neural Dev January 4, 2010; 5 1.

The F-box protein Cdc4/Fbxw7 is a novel regulator of neural crest development in Xenopus laevis.

Almeida AD , Wise HM , Hindley CJ , Slevin MK , Hartley RS , Philpott A .


Abstract
The neural crest is a unique population of cells that arise in the vertebrate ectoderm at the neural plate border after which they migrate extensively throughout the embryo, giving rise to a wide range of derivatives. A number of proteins involved in neural crest development have dynamic expression patterns, and it is becoming clear that ubiquitin-mediated protein degradation is partly responsible for this. Here we demonstrate a novel role for the F-box protein Cdc4/Fbxw7 in neural crest development. Two isoforms of Xenopus laevis Cdc4 were identified, and designated xCdc4alpha and xCdc4beta. These are highly conserved with vertebrate Cdc4 orthologs, and the Xenopus proteins are functionally equivalent in terms of their ability to degrade Cyclin E, an established vertebrate Cdc4 target. Blocking xCdc4 function specifically inhibited neural crest development at an early stage, prior to expression of c-Myc, Snail2 and Snail. We demonstrate that Cdc4, an ubiquitin E3 ligase subunit previously identified as targeting primarily cell cycle regulators for proteolysis, has additional roles in control of formation of the neural crest. Hence, we identify Cdc4 as a protein with separable but complementary functions in control of cell proliferation and differentiation.

PubMed ID: 20047651
PMC ID: PMC2819241
Article link: Neural Dev
Grant support: [+]
Genes referenced: actl6a fbxw7 gal.2 h3-3a msx1 myc npb pax3 plp1 six1 snai1 snai2 sox10 sox2 tnc zic1
Antibodies: Fbxw7 Ab1 Fbxw7 Ab2 H3f3a Ab9


Article Images: [+] show captions
References:
Anderson, 1997, Pubmed, Xenbase [+]


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