Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2010 Mar 12;28511:8408-21. doi: 10.1074/jbc.M109.064386.
Show Gene links
Show Anatomy links
Reversal of Xenopus Oct25 function by disruption of the POU domain structure.
Cao Y
,
Oswald F
,
Wacker SA
,
Bundschu K
,
Knöchel W
.
???displayArticle.abstract???
Xenopus Oct25 is a POU family subclass V (POU-V) transcription factor with a distinct domain structure. To investigate the contribution of different domains to the function of Oct25, we have performed gain of function analyses. Deletions of the N- or C-terminal regions and of the Hox domain (except its nuclear localization signal) result in mutants being indistinguishable from the wild type protein in the suppression of genes promoting germ layer formation. Deletion of the complete POU domain generates a mutant that has no effect on embryogenesis. However, disruption of the alpha-helical structures in the POU domain, even by a single amino acid mutation, causes reversal of protein function. Overexpression of such mutants leads to dorsalization of embryos and formation of secondary axial structures. The underlying mechanism is an enhanced transcription of genes coding for antagonists of the ligands for ventralizing bone morphogenetic protein and Wnt pathways. Corresponding deletion mutants of Xenopus Oct60, Oct91, or mouse Oct4 also exhibit such a dominant-negative effect. Therefore, our results reveal that the integrity of the POU domain is crucial for the function of POU-V transcription factors in the regulation of genes that promote germ layer formation.
Agius,
Endodermal Nodal-related signals and mesoderm induction in Xenopus.
2000, Pubmed,
Xenbase
Agius,
Endodermal Nodal-related signals and mesoderm induction in Xenopus.
2000,
Pubmed
,
Xenbase
Ambrosetti,
Modulation of the activity of multiple transcriptional activation domains by the DNA binding domains mediates the synergistic action of Sox2 and Oct-3 on the fibroblast growth factor-4 enhancer.
2000,
Pubmed
Atlasi,
OCT4 spliced variants are differentially expressed in human pluripotent and nonpluripotent cells.
2008,
Pubmed
Behrens,
Functional interaction of beta-catenin with the transcription factor LEF-1.
1996,
Pubmed
,
Xenbase
Boiani,
Regulatory networks in embryo-derived pluripotent stem cells.
2005,
Pubmed
Cao,
Xenopus POU factors of subclass V inhibit activin/nodal signaling during gastrulation.
2006,
Pubmed
,
Xenbase
Cao,
POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos.
2007,
Pubmed
,
Xenbase
Cao,
Oct25 represses transcription of nodal/activin target genes by interaction with signal transducers during Xenopus gastrulation.
2008,
Pubmed
,
Xenbase
Cao,
The POU factor Oct-25 regulates the Xvent-2B gene and counteracts terminal differentiation in Xenopus embryos.
2004,
Pubmed
,
Xenbase
Clements,
Mode of action of VegT in mesoderm and endoderm formation.
1999,
Pubmed
,
Xenbase
De Robertis,
The establishment of Spemann's organizer and patterning of the vertebrate embryo.
2000,
Pubmed
,
Xenbase
Frisch,
XBMPRII, a novel Xenopus type II receptor mediating BMP signaling in embryonic tissues.
1998,
Pubmed
,
Xenbase
Harland,
In situ hybridization: an improved whole-mount method for Xenopus embryos.
1991,
Pubmed
,
Xenbase
Hemmati-Brivanlou,
A truncated activin receptor inhibits mesoderm induction and formation of axial structures in Xenopus embryos.
1992,
Pubmed
,
Xenbase
Hinkley,
Sequential expression of multiple POU proteins during amphibian early development.
1992,
Pubmed
,
Xenbase
Hussain,
Sequence variation in the human transcription factor gene POU5F1.
2008,
Pubmed
Hyde,
Regulation of the early expression of the Xenopus nodal-related 1 gene, Xnr1.
2000,
Pubmed
,
Xenbase
Imagawa,
Stringent integrity requirements for both trans-activation and DNA-binding in a trans-activator, Oct3.
1991,
Pubmed
Jacobson,
Structure of Pit-1 POU domain bound to DNA as a dimer: unexpected arrangement and flexibility.
1997,
Pubmed
Jaenisch,
Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming.
2008,
Pubmed
Kim,
Oct4-induced pluripotency in adult neural stem cells.
2009,
Pubmed
McKendry,
LEF-1/TCF proteins mediate wnt-inducible transcription from the Xenopus nodal-related 3 promoter.
1997,
Pubmed
,
Xenbase
Molenaar,
XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos.
1996,
Pubmed
,
Xenbase
Morrison,
Conserved roles for Oct4 homologues in maintaining multipotency during early vertebrate development.
2006,
Pubmed
,
Xenbase
Nichols,
Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4.
1998,
Pubmed
Nishimoto,
The embryonic Octamer factor 3/4 displays distinct DNA binding specificity from those of other Octamer factors.
2003,
Pubmed
Niwa,
Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells.
2002,
Pubmed
Okamoto,
A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells.
1990,
Pubmed
Okumura-Nakanishi,
Oct-3/4 and Sox2 regulate Oct-3/4 gene in embryonic stem cells.
2005,
Pubmed
Pan,
Stem cell pluripotency and transcription factor Oct4.
2002,
Pubmed
Pan,
Identification of a nuclear localization signal in OCT4 and generation of a dominant negative mutant by its ablation.
2004,
Pubmed
Park,
Reprogramming of human somatic cells to pluripotency with defined factors.
2008,
Pubmed
Pesce,
Oct-4: gatekeeper in the beginnings of mammalian development.
2001,
Pubmed
Ring,
The role of a Williams-Beuren syndrome-associated helix-loop-helix domain-containing transcription factor in activin/nodal signaling.
2002,
Pubmed
,
Xenbase
Takahashi,
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
2006,
Pubmed
Takahashi,
Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
2007,
Pubmed
Takahashi,
Two novel nodal-related genes initiate early inductive events in Xenopus Nieuwkoop center.
2000,
Pubmed
,
Xenbase
Vigano,
Transcriptional activation by Oct-3: evidence for a specific role of the POU-specific domain in mediating functional interaction with Oct-1.
1996,
Pubmed
Wacker,
The initiation of Hox gene expression in Xenopus laevis is controlled by Brachyury and BMP-4.
2004,
Pubmed
,
Xenbase
Wegner,
POU-domain proteins: structure and function of developmental regulators.
1993,
Pubmed
Wernig,
In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state.
2007,
Pubmed
Wessely,
Neural induction in the absence of mesoderm: beta-catenin-dependent expression of secreted BMP antagonists at the blastula stage in Xenopus.
2001,
Pubmed
,
Xenbase
White,
Direct and indirect regulation of derrière, a Xenopus mesoderm-inducing factor, by VegT.
2002,
Pubmed
,
Xenbase
Whitfield,
XLPOU-60, a Xenopus POU-domain mRNA, is oocyte-specific from very early stages of oogenesis, and localised to presumptive mesoderm and ectoderm in the blastula.
1993,
Pubmed
,
Xenbase
Whitfield,
Early embryonic expression of XLPOU-60, a Xenopus POU-domain protein.
1995,
Pubmed
,
Xenbase
Wodarz,
Mechanisms of Wnt signaling in development.
1998,
Pubmed
,
Xenbase
Yu,
Induced pluripotent stem cell lines derived from human somatic cells.
2007,
Pubmed
