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XB-ART-40937
Dev Biol March 15, 2010; 339 (2): 494-506.

Regulation of photoreceptor gene expression by the retinal homeobox (Rx) gene product.

Pan Y , Martinez-De Luna RI , Lou CH , Nekkalapudi S , Kelly LE , Sater AK , El-Hodiri HM .


Abstract
The retinal homeobox (Rx) gene product is essential for eye development. However little is known about its molecular function. It has been demonstrated that Rx binds to photoreceptor conserved element (PCE-1), a highly conserved element found in the promoter region of photoreceptor-specific genes such as rhodopsin and red cone opsin. We verify that Rx is co-expressed with rhodopsin and red cone opsin in maturing photoreceptors and demonstrate that Rx binds to the rhodopsin and red cone opsin promoters in vivo. We also find that Rx can cooperate with the Xenopus analogs of Crx and Nrl, otx5b and XLMaf (respectively), to activate a Xenopus opsin promoter-dependent reporter. Finally, we demonstrate that reduction of Rx expression in tadpoles results in decreases in expression of several PCE-1 containing photoreceptor genes, abnormal photoreceptor morphology, and impaired vision. Our data suggests that Rx, in combination with other transcription factors, is necessary for normal photoreceptor gene expression, maintenance, and function. This establishes a direct role for Rx in regulation of genes expressed in a differentiated cell type.

PubMed ID: 20060393
PMC ID: PMC2830336
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus
Genes referenced: ago2 ces3.7 crx myc nrl opn1lw pmel rax rho rpe
Antibodies: Isl1/2 Ab1 Peanut Agglutinin Rho Ab2


Article Images: [+] show captions
References [+] :
Andreazzoli, Role of Xrx1 in Xenopus eye and anterior brain development. 1999, Pubmed, Xenbase