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A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis.
Honsbein A
,
Sokolovski S
,
Grefen C
,
Campanoni P
,
Pratelli R
,
Paneque M
,
Chen Z
,
Johansson I
,
Blatt MR
.
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A few membrane vesicle trafficking (SNARE) proteins in plants are associated with signaling and transmembrane ion transport, including control of plasma membrane ion channels. Vesicle traffic contributes to the population of ion channels at the plasma membrane. Nonetheless, it is unclear whether these SNAREs also interact directly to affect channel gating and, if so, what functional impact this might have on the plant. Here, we report that the Arabidopsis thaliana SNARE SYP121 binds to KC1, a regulatory K(+) channel subunit that assembles with different inward-rectifying K(+) channels to affect their activities. We demonstrate that SYP121 interacts preferentially with KC1 over other Kv-like K(+) channel subunits and that KC1 interacts specifically with SYP121 but not with its closest structural and functional homolog SYP122 nor with another related SNARE SYP111. SYP121 promoted gating of the inward-rectifying K(+) channel AKT1 but only when heterologously coexpressed with KC1. Mutation in any one of the three genes, SYP121, KC1, and AKT1, selectively suppressed the inward-rectifying K(+) current in Arabidopsis root epidermal protoplasts as well as K(+) acquisition and growth in seedlings when channel-mediated K(+) uptake was limiting. That SYP121 should be important for gating of a K(+) channel and its role in inorganic mineral nutrition demonstrates an unexpected role for SNARE-ion channel interactions, apparently divorced from signaling and vesicle traffic. Instead, it suggests a role in regulating K(+) uptake coordinately with membrane expansion for cell growth.
Alexandersson,
Arabidopsis plasma membrane proteomics identifies components of transport, signal transduction and membrane trafficking.
2004, Pubmed
Alexandersson,
Arabidopsis plasma membrane proteomics identifies components of transport, signal transduction and membrane trafficking.
2004,
Pubmed
Amtmann,
Regulation of macronutrient transport.
2009,
Pubmed
Ashley,
Plant responses to potassium deficiencies: a role for potassium transport proteins.
2006,
Pubmed
Assaad,
The PEN1 syntaxin defines a novel cellular compartment upon fungal attack and is required for the timely assembly of papillae.
2004,
Pubmed
Bassham,
SNAREs: cogs and coordinators in signaling and development.
2008,
Pubmed
Birnbaum,
A gene expression map of the Arabidopsis root.
2003,
Pubmed
Blatt,
K+ channels of Cf-9 transgenic tobacco guard cells as targets for Cladosporium fulvum Avr9 elicitor-dependent signal transduction.
1999,
Pubmed
Brunger,
Structure and function of SNARE and SNARE-interacting proteins.
2005,
Pubmed
Bryant,
Regulated transport of the glucose transporter GLUT4.
2002,
Pubmed
Buschmann,
Enhancement of Na(+) uptake currents, time-dependent inward-rectifying K(+) channel currents, and K(+) channel transcripts by K(+) starvation in wheat root cells.
2000,
Pubmed
Campanoni,
A generalized method for transfecting root epidermis uncovers endosomal dynamics in Arabidopsis root hairs.
2007,
Pubmed
Campanoni,
Membrane trafficking and polar growth in root hairs and pollen tubes.
2007,
Pubmed
Collins,
SNARE-protein-mediated disease resistance at the plant cell wall.
2003,
Pubmed
Dhonukshe,
Generation of cell polarity in plants links endocytosis, auxin distribution and cell fate decisions.
2008,
Pubmed
Dreyer,
Plant K+ channel alpha-subunits assemble indiscriminately.
1997,
Pubmed
,
Xenbase
Dreyer,
What makes a gate? The ins and outs of Kv-like K+ channels in plants.
2009,
Pubmed
Duby,
AtKC1, a conditionally targeted Shaker-type subunit, regulates the activity of plant K+ channels.
2008,
Pubmed
Fletcher,
False interaction of syntaxin 1A with a Ca(2+)-activated K(+) channel revealed by co-immunoprecipitation and pull-down assays: implications for identification of protein-protein interactions.
2003,
Pubmed
Gassmann,
Inward-Rectifying K+ Channels in Root Hairs of Wheat (A Mechanism for Aluminum-Sensitive Low-Affinity K+ Uptake and Membrane Potential Control).
1994,
Pubmed
Gaymard,
The baculovirus/insect cell system as an alternative to Xenopus oocytes. First characterization of the AKT1 K+ channel from Arabidopsis thaliana.
1996,
Pubmed
,
Xenbase
Geelen,
The abscisic acid-related SNARE homolog NtSyr1 contributes to secretion and growth: evidence from competition with its cytosolic domain.
2002,
Pubmed
Gierth,
The potassium transporter AtHAK5 functions in K(+) deprivation-induced high-affinity K(+) uptake and AKT1 K(+) channel contribution to K(+) uptake kinetics in Arabidopsis roots.
2005,
Pubmed
Grefen,
The determination of protein-protein interactions by the mating-based split-ubiquitin system (mbSUS).
2009,
Pubmed
Grefen,
SNAREs--molecular governors in signalling and development.
2008,
Pubmed
Hetherington,
The role of stomata in sensing and driving environmental change.
2003,
Pubmed
Heusser,
Trafficking of potassium channels.
2005,
Pubmed
Hirsch,
A role for the AKT1 potassium channel in plant nutrition.
1998,
Pubmed
Hu,
Fusion of cells by flipped SNAREs.
2003,
Pubmed
Ivashikina,
K(+) channel profile and electrical properties of Arabidopsis root hairs.
2001,
Pubmed
Jahn,
Membrane fusion.
2003,
Pubmed
Johansson,
External K+ modulates the activity of the Arabidopsis potassium channel SKOR via an unusual mechanism.
2006,
Pubmed
Jürgens,
Cytokinesis in higher plants.
2005,
Pubmed
Kato,
SGR2, a phospholipase-like protein, and ZIG/SGR4, a SNARE, are involved in the shoot gravitropism of Arabidopsis.
2002,
Pubmed
Kwon,
Co-option of a default secretory pathway for plant immune responses.
2008,
Pubmed
Lagarde,
Tissue-specific expression of Arabidopsis AKT1 gene is consistent with a role in K+ nutrition.
1996,
Pubmed
Leung,
SNAREing voltage-gated K+ and ATP-sensitive K+ channels: tuning beta-cell excitability with syntaxin-1A and other exocytotic proteins.
2007,
Pubmed
Leyman,
A tobacco syntaxin with a role in hormonal control of guard cell ion channels.
1999,
Pubmed
Li,
A Ca(2)+ signaling pathway regulates a K(+) channel for low-K response in Arabidopsis.
2006,
Pubmed
,
Xenbase
Lipka,
SNARE-ware: the role of SNARE-domain proteins in plant biology.
2007,
Pubmed
Ma,
Diverse trafficking patterns due to multiple traffic motifs in G protein-activated inwardly rectifying potassium channels from brain and heart.
2002,
Pubmed
Maathuis,
Transcriptome analysis of root transporters reveals participation of multiple gene families in the response to cation stress.
2003,
Pubmed
Marmagne,
Identification of new intrinsic proteins in Arabidopsis plasma membrane proteome.
2004,
Pubmed
Obrdlik,
K+ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions.
2004,
Pubmed
Pajonk,
Activity determinants and functional specialization of Arabidopsis PEN1 syntaxin in innate immunity.
2008,
Pubmed
Pardo,
Structure of a plasma membrane H+-ATPase gene from the plant Arabidopsis thaliana.
1989,
Pubmed
Parlati,
Rapid and efficient fusion of phospholipid vesicles by the alpha-helical core of a SNARE complex in the absence of an N-terminal regulatory domain.
1999,
Pubmed
Paumet,
The specificity of SNARE-dependent fusion is encoded in the SNARE motif.
2004,
Pubmed
Reintanz,
AtKC1, a silent Arabidopsis potassium channel alpha -subunit modulates root hair K+ influx.
2002,
Pubmed
Robatzek,
Ligand-induced endocytosis of the pattern recognition receptor FLS2 in Arabidopsis.
2006,
Pubmed
Saito,
Amyloplasts and vacuolar membrane dynamics in the living graviperceptive cell of the Arabidopsis inflorescence stem.
2005,
Pubmed
Sokolovski,
Functional interaction of the SNARE protein NtSyp121 in Ca2+ channel gating, Ca2+ transients and ABA signalling of stomatal guard cells.
2008,
Pubmed
Sottocornola,
The potassium channel KAT1 is activated by plant and animal 14-3-3 proteins.
2006,
Pubmed
,
Xenbase
Spalding,
Potassium uptake supporting plant growth in the absence of AKT1 channel activity: Inhibition by ammonium and stimulation by sodium.
1999,
Pubmed
Steinmann,
Coordinated polar localization of auxin efflux carrier PIN1 by GNOM ARF GEF.
1999,
Pubmed
Sutter,
Selective mobility and sensitivity to SNAREs is exhibited by the Arabidopsis KAT1 K+ channel at the plasma membrane.
2006,
Pubmed
,
Xenbase
Sutter,
Setting SNAREs in a different wood.
2006,
Pubmed
Sutter,
Abscisic acid triggers the endocytosis of the arabidopsis KAT1 K+ channel and its recycling to the plasma membrane.
2007,
Pubmed
Szyroki,
KAT1 is not essential for stomatal opening.
2001,
Pubmed
Takano,
Endocytosis and degradation of BOR1, a boron transporter of Arabidopsis thaliana, regulated by boron availability.
2005,
Pubmed
Tsuk,
Kv2.1 channel activation and inactivation is influenced by physical interactions of both syntaxin 1A and the syntaxin 1A/soluble N-ethylmaleimide-sensitive factor-25 (t-SNARE) complex with the C terminus of the channel.
2005,
Pubmed
,
Xenbase
Tyrrell,
Selective targeting of plasma membrane and tonoplast traffic by inhibitory (dominant-negative) SNARE fragments.
2007,
Pubmed
Uemura,
Systematic analysis of SNARE molecules in Arabidopsis: dissection of the post-Golgi network in plant cells.
2004,
Pubmed
Ungar,
SNARE protein structure and function.
2003,
Pubmed
Varlamov,
i-SNAREs: inhibitory SNAREs that fine-tune the specificity of membrane fusion.
2004,
Pubmed
Véry,
Molecular mechanisms and regulation of K+ transport in higher plants.
2003,
Pubmed
Walter,
Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation.
2004,
Pubmed
Weber,
SNAREpins: minimal machinery for membrane fusion.
1998,
Pubmed
Xu,
A protein kinase, interacting with two calcineurin B-like proteins, regulates K+ transporter AKT1 in Arabidopsis.
2006,
Pubmed
,
Xenbase
Yano,
A SNARE complex containing SGR3/AtVAM3 and ZIG/VTI11 in gravity-sensing cells is important for Arabidopsis shoot gravitropism.
2003,
Pubmed
Yuan,
14-3-3 dimers probe the assembly status of multimeric membrane proteins.
2003,
Pubmed
Yuasa,
Membrane-anchored prolyl hydroxylase with an export signal from the endoplasmic reticulum.
2005,
Pubmed
Zhang,
A SNARE-protein has opposing functions in penetration resistance and defence signalling pathways.
2007,
Pubmed
