Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-40988
Methods 2010 May 01;511:37-44. doi: 10.1016/j.ymeth.2010.01.014.
Show Gene links Show Anatomy links

Tips and tricks for preparing lampbrush chromosome spreads from Xenopus tropicalis oocytes.

Penrad-Mobayed M , Kanhoush R , Perrin C .


???displayArticle.abstract???
Due to their large size and fine organization, lampbrush chromosomes (LBCs) of amphibian oocytes have been for decades one of the favorite tools of biologists for the analysis of transcriptional and post-transcriptional processes at the cytological level. The emergence of the diploid Xenopus tropicalis amphibian as a model organism for vertebrate developmental genetics and the accumulation of sequence data made available by its recent genomic sequencing, strongly revive the interest of LBCs as a powerful tool to study genes expressed during oogenesis. We describe here a detailed protocol for preparing LBCs from X. tropicalis oocyte and give practical advice to encourage a large number of researchers to become familiar with these chromosomes.

???displayArticle.pubmedLink??? 20085818
???displayArticle.link??? Methods


Species referenced: Xenopus tropicalis
???displayArticle.antibodies??? Xnf7 Ab1