Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Immunogenetics
2004 Feb 01;5511:770-81. doi: 10.1007/s00251-003-0635-0.
Show Gene links
Show Anatomy links
Analysis of genomic and expressed major histocompatibility class Ia and class II genes in a hexaploid Lake Tana African 'large' barb individual (Barbus intermedius).
Kruiswijk CP
,
Hermsen T
,
Fujiki K
,
Dixon B
,
Savelkoul HF
,
Stet RJ
.
???displayArticle.abstract???
Expression of too many co-dominant major histocompatibility complex (MHC) alleles is thought to be detrimental to proper functioning of the immune system. Polyploidy of the genome will increase the number of expressed MHC genes unless they are prone to a silencing mechanism. In polyploid Xenopus species, the number of MHC class I and II genes has been physically reduced, as it does not increase with higher ploidy genomes. In the zebrafish some class II B loci have been silenced, as only two genomically bona fide loci, DAA/DAB and DEA/DEB, have been described. Earlier studies indicated a reduction in the number of genomic and expressed class II MHC genes in a hexaploid African 'large' barb. This prompted us to study the number of MHC genes present in the genome of an African 'large' barb individual (Barbus intermedius) in relation to those expressed, adopting the following strategy. Full-length cDNA sequences were generated from mRNA and compared with partial genomic class Ia and II sequences generated by PCR using the same primer set. In addition, we performed Southern hybridizations to obtain a verification of the number of class I and II B genes. Our study revealed three beta2-microglobulin, five class Ia, four class II A, and four class II B genes at the genomic level, which were shown to be expressed in the hexaploid barb individual. The class Ia and class II data indicate that the ploidy status does not correlate with the presence and expression of these MHC genes.
Bartl,
Identification of class I genes in cartilaginous fish, the most ancient group of vertebrates displaying an adaptive immune response.
1997, Pubmed
Bartl,
Identification of class I genes in cartilaginous fish, the most ancient group of vertebrates displaying an adaptive immune response.
1997,
Pubmed
Betz,
Major histocompatibility complex class I genes of the coelacanth Latimeria chalumnae.
1994,
Pubmed
,
Xenbase
Bingulac-Popovic,
Mapping of mhc class I and class II regions to different linkage groups in the zebrafish, Danio rerio.
1997,
Pubmed
Bjorkman,
Structure of the human class I histocompatibility antigen, HLA-A2.
,
Pubmed
Criscitiello,
Beta 2-microglobulin of ictalurid catfishes.
1998,
Pubmed
Dixon,
Characterization of beta 2-microglobulin transcripts from two teleost species.
1993,
Pubmed
Dixon,
Fish major histocompatibility complex genes: an expansion.
1995,
Pubmed
Dixon,
Evolution of MHC class II beta chain-encoding genes in the Lake Tana barbel species flock (Barbus intermedius complex).
1996,
Pubmed
Flajnik,
A novel type of class I gene organization in vertebrates: a large family of non-MHC-linked class I genes is expressed at the RNA level in the amphibian Xenopus.
1993,
Pubmed
,
Xenbase
Fujiki,
Cloning and characterization of cDNA clones encoding membrane-bound and potentially secreted major histocompatibility class I receptors from walleye (Stizostedion vitreum).
2001,
Pubmed
Güssow,
The human beta 2-microglobulin gene. Primary structure and definition of the transcriptional unit.
1987,
Pubmed
Kasahara,
Evolution of the major histocompatibility complex: a current overview.
1995,
Pubmed
Kaufman,
Evolutionary conservation of MHC class I and class II molecules--different yet the same.
1994,
Pubmed
Kaufman,
Different features of the MHC class I heterodimer have evolved at different rates. Chicken B-F and beta 2-microglobulin sequences reveal invariant surface residues.
1992,
Pubmed
Klein,
Origin of major histocompatibility complex polymorphism: the trans-species hypothesis.
1987,
Pubmed
Klein,
Evolution of the major histocompatibility complex.
1986,
Pubmed
Kruiswijk,
A novel functional class I lineage in zebrafish (Danio rerio), carp (Cyprinus carpio), and large barbus (Barbus intermedius) showing an unusual conservation of the peptide binding domains.
2002,
Pubmed
Kumar,
MEGA2: molecular evolutionary genetics analysis software.
2001,
Pubmed
Lawlor,
Evolution of class-I MHC genes and proteins: from natural selection to thymic selection.
1990,
Pubmed
Lundqvist,
Characterization of beta2-microglobulin in a primitive fish, the Siberian sturgeon (Acipenser baeri).
1999,
Pubmed
Michalová,
A contig map of the Mhc class I genomic region in the zebrafish reveals ancient synteny.
2000,
Pubmed
Murray,
Linkage Relationships and Haplotype Variation of the Major Histocompatibility Complex Class I A Genes in the Cichlid Fish Oreochromis niloticus.
2000,
Pubmed
Müller,
DXS106 and DXS559 flank the X-linked dystonia-parkinsonism syndrome locus (DYT3).
1994,
Pubmed
Nakai,
PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization.
1999,
Pubmed
Ono,
New beta chain-encoding Mhc class II genes in the carp.
1993,
Pubmed
Ono,
Cloning of the beta 2-microglobulin gene in the zebrafish.
1993,
Pubmed
Parnes,
Multiple mRNA species with distinct 3' termini are transcribed from the beta 2-microglobulin gene.
,
Pubmed
Persson,
Characterization of MHC class I and beta(2)-microglobulin sequences in Atlantic cod reveals an unusually high number of expressed class I genes.
1999,
Pubmed
Saitou,
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
1987,
Pubmed
Salter,
A binding site for the T-cell co-receptor CD8 on the alpha 3 domain of HLA-A2.
1990,
Pubmed
Sammut,
The fate of duplicated major histocompatibility complex class Ia genes in a dodecaploid amphibian, Xenopus ruwenzoriensis.
2002,
Pubmed
,
Xenbase
Shum,
Isolation of a classical MHC class I cDNA from an amphibian. Evidence for only one class I locus in the Xenopus MHC.
1993,
Pubmed
,
Xenbase
Shum,
Modes of salmonid MHC class I and II evolution differ from the primate paradigm.
2001,
Pubmed
Shum,
Unexpected beta2-microglobulin sequence diversity in individual rainbow trout.
1996,
Pubmed
Shum,
Structures of two major histocompatibility complex class I genes of the rainbow trout (Oncorhynchus mykiss).
2002,
Pubmed
Stet,
Major histocompatibility genes in cyprinid fishes: theory and practice.
1998,
Pubmed
Thompson,
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
1997,
Pubmed
Zardoya,
Molecular evidence on the evolutionary and biogeographical patterns of European cyprinids.
1999,
Pubmed
van Erp,
Characterization of class II A and B genes in a gynogenetic carp clone.
1996,
Pubmed
