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Nature
2009 Dec 10;4627274:757-61. doi: 10.1038/nature08558.
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Crystal structure of a bacterial homologue of the kidney urea transporter.
Levin EJ
,
Quick M
,
Zhou M
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Urea is highly concentrated in the mammalian kidney to produce the osmotic gradient necessary for water re-absorption. Free diffusion of urea across cell membranes is slow owing to its high polarity, and specialized urea transporters have evolved to achieve rapid and selective urea permeation. Here we present the 2.3 A structure of a functional urea transporter from the bacterium Desulfovibrio vulgaris. The transporter is a homotrimer, and each subunit contains a continuous membrane-spanning pore formed by the two homologous halves of the protein. The pore contains a constricted selectivity filter that can accommodate several dehydrated urea molecules in single file. Backbone and side-chain oxygen atoms provide continuous coordination of urea as it progresses through the filter, and well-placed alpha-helix dipoles provide further compensation for dehydration energy. These results establish that the urea transporter operates by a channel-like mechanism and reveal the physical and chemical basis of urea selectivity.
Bagnasco,
Role and regulation of urea transporters.
2005, Pubmed
Bagnasco,
Role and regulation of urea transporters.
2005,
Pubmed
Blount,
Forskolin stimulates phosphorylation and membrane accumulation of UT-A3.
2007,
Pubmed
Bradford,
97- and 117-kDa forms of collecting duct urea transporter UT-A1 are due to different states of glycosylation.
2001,
Pubmed
Brünger,
Crystallography & NMR system: A new software suite for macromolecular structure determination.
1998,
Pubmed
Chou,
Inhibition of urea transport in inner medullary collecting duct by phloretin and urea analogues.
1989,
Pubmed
Collaborative Computational Project, Number 4,
The CCP4 suite: programs for protein crystallography.
1994,
Pubmed
Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed
Dutzler,
X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity.
2002,
Pubmed
Emsley,
Coot: model-building tools for molecular graphics.
2004,
Pubmed
Finkelstein,
Water and nonelectrolyte permeability of lipid bilayer membranes.
1976,
Pubmed
Fu,
Structure of a glycerol-conducting channel and the basis for its selectivity.
2000,
Pubmed
Hediger,
Structure, regulation and physiological roles of urea transporters.
1996,
Pubmed
,
Xenbase
Imai,
Amide-pi interactions between formamide and benzene.
2009,
Pubmed
Khademi,
Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A.
2004,
Pubmed
Knepper,
Structural biology: Molecular coin slots for urea.
2009,
Pubmed
Kojima,
Molecular mechanisms of urea transport in plants.
2006,
Pubmed
Lebedev,
Model preparation in MOLREP and examples of model improvement using X-ray data.
2008,
Pubmed
Levin,
Urearetics: a small molecule screen yields nanomolar potency inhibitors of urea transporter UT-B.
2007,
Pubmed
Lucien,
Antigenic and functional properties of the human red blood cell urea transporter hUT-B1.
2002,
Pubmed
,
Xenbase
Maciver,
Functional characterization of mouse urea transporters UT-A2 and UT-A3 expressed in purified Xenopus laevis oocyte plasma membranes.
2008,
Pubmed
,
Xenbase
Mannuzzu,
Estimate of the number of urea transport sites in erythrocyte ghosts using a hydrophobic mercurial.
1993,
Pubmed
McCoy,
Phaser crystallographic software.
2007,
Pubmed
Minocha,
The urea transporter (UT) family: bioinformatic analyses leading to structural, functional, and evolutionary predictions.
2003,
Pubmed
Murata,
Structural determinants of water permeation through aquaporin-1.
2000,
Pubmed
Otwinowski,
Processing of X-ray diffraction data collected in oscillation mode.
1997,
Pubmed
Potterton,
Developments in the CCP4 molecular-graphics project.
2004,
Pubmed
Quick,
Monitoring the function of membrane transport proteins in detergent-solubilized form.
2007,
Pubmed
Raunser,
Oligomeric structure and functional characterization of the urea transporter from Actinobacillus pleuropneumoniae.
2009,
Pubmed
Sands,
Mammalian urea transporters.
2003,
Pubmed
Sebbane,
The Yersinia pseudotuberculosis Yut protein, a new type of urea transporter homologous to eukaryotic channels and functionally interchangeable in vitro with the Helicobacter pylori UreI protein.
2002,
Pubmed
,
Xenbase
Shayakul,
Molecular cloning and characterization of the vasopressin-regulated urea transporter of rat kidney collecting ducts.
1996,
Pubmed
,
Xenbase
Shayakul,
The SLC14 gene family of urea transporters.
2004,
Pubmed
Shi,
The mechanism of a neurotransmitter:sodium symporter--inward release of Na+ and substrate is triggered by substrate in a second binding site.
2008,
Pubmed
Sui,
Structural basis of water-specific transport through the AQP1 water channel.
,
Pubmed
Terris,
UT-A3: localization and characterization of an additional urea transporter isoform in the IMCD.
2001,
Pubmed
Terwilliger,
Automated MAD and MIR structure solution.
1999,
Pubmed
Vagin,
REFMAC5 dictionary: organization of prior chemical knowledge and guidelines for its use.
2004,
Pubmed
Valladares,
An ABC-type, high-affinity urea permease identified in cyanobacteria.
2002,
Pubmed
Weeks,
A H+-gated urea channel: the link between Helicobacter pylori urease and gastric colonization.
2000,
Pubmed
,
Xenbase
Winn,
Use of TLS parameters to model anisotropic displacements in macromolecular refinement.
2001,
Pubmed
Yang,
Analysis of double knockout mice lacking aquaporin-1 and urea transporter UT-B. Evidence for UT-B-facilitated water transport in erythrocytes.
2002,
Pubmed
,
Xenbase
You,
Cloning and characterization of the vasopressin-regulated urea transporter.
1993,
Pubmed
,
Xenbase
Zhang,
Vasopressin rapidly increases phosphorylation of UT-A1 urea transporter in rat IMCDs through PKA.
2002,
Pubmed
Zhao,
Comparative transport efficiencies of urea analogues through urea transporter UT-B.
2007,
Pubmed
Zheng,
The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli.
2004,
Pubmed
von Heijne,
Topogenic signals in integral membrane proteins.
1988,
Pubmed
