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XB-ART-41230
Dev Biol. May 1, 2010; 341 (1): 222-35.

FMR1/FXR1 and the miRNA pathway are required for eye and neural crest development.

Gessert S , Bugner V , Tecza A , Pinker M , Kühl M .


Abstract
FMR1 and FXR1 are RNA binding proteins interacting with the miRNA-induced silencing complex, RISC. Here we describe for the first time the function of these proteins during eye and neural crest (NC) development in Xenopus laevis. A loss of FMR1 or FXR1 results in abnormal eye development as well as defects in cranial cartilage derived from cranial NC cells. We further investigated the possible mechanism of these phenotypes by showing that a depletion of Dicer, an important enzyme for generating all mature miRNAs, in the anterior neural tissue also leads to eye and cranial cartilage defects. Furthermore, we examined the function of 12 miRNAs during anterior neural development. We show a specific requirement of six selected miRNAs during eye and cranial cartilage development. Mir-130a, -219, and -23b are involved in eye formation only whereas loss of miR-200b, miR-96 and miR-196a results in strong defects during eye as well as cranial cartilage development. Our results suggest an essential role for FMR1 and FXR1 for eye and NC development in X.laevis likely through an interaction with the miRNA pathway.

PubMed ID: 20197067
Article link: Dev Biol.

Genes referenced: dicer1 egr2 emx1 emx1.2 en2 fmr1 foxd3 fxr1 pax6 rax rpe snai2 sox3 tbx2 twist1

Morpholinos referenced: dicer1 MO3 dicer1 MO4 fmr1 MO1 fxr1 MO1 huwe1 MO1 huwe1 MO2 huwe1 MO3 id3 MO3 parp14.4 MO1 ska2 MO1


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