Strategies for enhanced annotation of a microarray probe set.
We aim to determine the biological relevance of genes identified through microarray-mediated transcriptional profiling of Xenopus sensory organs and brain. Difficulties with genetic data analysis arise because of limitations in probe set annotation and the lack of a universal gene nomenclature. To overcome these impediments, we used sequence based and semantic linking methods in combination with computational approaches to augment probe set annotation on a commercially available microarray. Our curation efforts enabled linkage of probe sets and expression data to public databases, increased the biological significance of our microarray data, and assisted with the tentative identification of unidentified probe sets.
PubMed ID: 20223738
PMC ID: PMC2955980
Article link: Int J Bioinform Res Appl.
Grant support: DC003292 NIDCD NIH HHS , GM008136 NIGMS NIH HHS , P50 GM068762-060005 NIGMS NIH HHS , P50 GM068762-070005 NIGMS NIH HHS , P50GM068762 NIGMS NIH HHS , R01 DC003292-06 NIDCD NIH HHS , R01 DC003292-07 NIDCD NIH HHS , S06 GM008136-330029 NIGMS NIH HHS , R25 GM061222 NIGMS NIH HHS