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XB-ART-41269
Dev Biol May 15, 2010; 341 (2): 375-88.

Neural crest migration requires the activity of the extracellular sulphatases XtSulf1 and XtSulf2.

Guiral EC , Faas L , Pownall ME .


Abstract
In vertebrates, there are two related genes, Sulf1 and Sulf2 that code for extracellular heparan sulphate 6-0-endosulphatases. These enzymes act to post-synthetically remodel heparan sulphate chains, generating structural diversity of cell surface HSPGs; this activity provides an important mechanism to modulate developmental cell signalling. Here we describe the expression and activity of Xenopus tropicalis Sulf2 (XtSulf2), which like XtSulf1, can act extracellularly to inhibit BMP4 and FGF4 signalling. Consistent with its discrete expression in regions of the anterior developing nervous system, we found that overexpression of XtSulf2 disrupts the expression of a set of neural markers and inhibits the migration of the neural crest. Using a combination of grafting experiments and antisense morpholino based knockdown studies in Xenopus embryos, we demonstrate that endogenous XtSulf1 and XtSulf2 play an important role during cranial neural crest cell migration in vivo.

PubMed ID: 20206618
Article link: Dev Biol
Grant support: [+]
Genes referenced: actc1 actl6a bmp4 bmpr1a egr2 en2 fgf3 fgf4 fgf8 fgfr1 hoxb3 isl1 lhx1 myc myod1 ncam1 neurod1 nucb1 otx2 pax6 snai2 sox3 sox8 sulf1 sulf2 twist1
Morpholinos: sulf1 MO1 sulf1 MO2 sulf1 MO3 sulf2 MO1


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