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XB-ART-41291
Dev Dyn April 1, 2010; 239 (4): 1178-87.

ETS family protein ETV2 is required for initiation of the endothelial lineage but not the hematopoietic lineage in the Xenopus embryo.

Salanga MC , Meadows SM , Myers CT , Krieg PA .


Abstract
Transcription factors of the ETS family are important regulators of endothelial and hematopoietic development. We have characterized the Xenopus orthologue of the ETS transcription factor, ETV2. Expression analysis shows that etv2 is highly expressed in hematopoietic and endothelial precursor cells in the Xenopus embryo. In gain-of-function experiments, ETV2 is sufficient to activate ectopic expression of vascular endothelial markers. In addition, ETV2 activated expression of hematopoietic genes representing the myeloid but not the erythroid lineage. Loss-of-function studies indicate that ETV2 is required for expression of all endothelial markers examined. However, knockdown of ETV2 has no detectable effects on expression of either myeloid or erythroid markers. This contrasts with studies in mouse and zebrafish where ETV2 is required for development of the myeloid lineage. Our studies confirm an essential role for ETV2 in endothelial development, but also reveal important differences in hematopoietic development between organisms.

PubMed ID: 20235229
PMC ID: PMC3269837
Article link: Dev Dyn
Grant support: [+]
Genes referenced: aplnr erg etv2 hba1 kdr runx1 spib
Morpholinos: etv2 MO1 etv2 MO2


Article Images: [+] show captions
References [+] :
Afouda, GATA transcription factors integrate Wnt signalling during heart development. 2008, Pubmed, Xenbase


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