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XB-ART-41484
Dev Dyn July 1, 2010; 239 (7): 1977-87.

Regulation of vertebrate embryogenesis by the exon junction complex core component Eif4a3.

Haremaki T , Sridharan J , Dvora S , Weinstein DC .


Abstract
The establishment and maintenance of cellular identity are ultimately dependent upon the accurate regulation of gene expression, the process by which genetic information is used to synthesize functional gene products. The post-transcriptional, pre-translational regulation of RNA constitutes RNA processing, which plays a prominent role in the modulation of gene expression in differentiated animal cells. The multi-protein Exon Junction Complex (EJC) serves as a critical signaling hub within the network that underlies many RNA processing events. Here, we identify a requirement for the EJC during early vertebrate embryogenesis. Knockdown of the EJC component Eukaryotic initiation factor 4a3 (Eif4a3) in embryos of the frog Xenopus laevis results in full-body paralysis, with defects in sensory neuron, pigment cell, and cardiac development; similar phenotypes are seen following knockdown of other "core" EJC protein constituents. Our studies point to an essential role for the EJC in the development of neural plate border derivatives.

PubMed ID: 20549732
PMC ID: PMC2922852
Article link: Dev Dyn
Grant support: [+]

Species referenced: Xenopus
Genes referenced: eif4a3.1 elavl2 gal.2 magoh nkx2-5 rbm8a
Antibodies: Casp3 Ab2 Isl1 Ab1 Neuronal Ab4 Somite Ab1
Morpholinos: eif4a3.1 MO1 eif4a3.1 MO2 magoh MO3 rbm8a MO1


Article Images: [+] show captions
References [+] :
Artinger, Zebrafish narrowminded suggests a genetic link between formation of neural crest and primary sensory neurons. 1999, Pubmed