Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Mol Cell Biol. July 1, 2010; 30 (14): 3620-34.

miR-31 functions as a negative regulator of lymphatic vascular lineage-specific differentiation in vitro and vascular development in vivo.

Pedrioli DM , Karpanen T , Dabouras V , Jurisic G , van de Hoek G , Shin JW , Marino D , Kälin RE , Leidel S , Cinelli P , Schulte-Merker S , Brändli AW , Detmar M .

The lymphatic vascular system maintains tissue fluid homeostasis, helps mediate afferent immune responses, and promotes cancer metastasis. To address the role microRNAs (miRNAs) play in the development and function of the lymphatic vascular system, we defined the in vitro miRNA expression profiles of primary human lymphatic endothelial cells (LECs) and blood vascular endothelial cells (BVECs) and identified four BVEC signature and two LEC signature miRNAs. Their vascular lineage-specific expression patterns were confirmed in vivo by quantitative real-time PCR and in situ hybridization. Functional characterization of the BVEC signature miRNA miR-31 identified a novel BVEC-specific posttranscriptional regulatory mechanism that inhibits the expression of lymphatic lineage-specific transcripts in vitro. We demonstrate that suppression of lymphatic differentiation is partially mediated via direct repression of PROX1, a transcription factor that functions as a master regulator of lymphatic lineage-specific differentiation. Finally, in vivo studies of Xenopus and zebrafish demonstrated that gain of miR-31 function impaired venous sprouting and lymphatic vascular development, thus highlighting the importance of miR-31 as a negative regulator of lymphatic development. Collectively, our findings identify miR-31 is a potent regulator of vascular lineage-specific differentiation and development in vertebrates.

PubMed ID: 20479124
PMC ID: PMC2897549
Article link: Mol Cell Biol.
Grant support: CA69184 NCI NIH HHS , R01 CA069184 NCI NIH HHS , CA69184 NCI NIH HHS

Genes referenced: prox1 vegfc

Morpholinos referenced: vegfc MO2

Adams, 2007, Pubmed[+]

My Xenbase: [ Log-in / Register ]
version: [4.4.0]

Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556