Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2010 May 04;10718:8440-5. doi: 10.1073/pnas.1001425107.
Show Gene links
Show Anatomy links
Drosophila TRPA1 channel mediates chemical avoidance in gustatory receptor neurons.
Kim SH
,
Lee Y
,
Akitake B
,
Woodward OM
,
Guggino WB
,
Montell C
.
???displayArticle.abstract???
Mammalian sweet, bitter, and umami taste is mediated by a single transduction pathway that includes a phospholipase C (PLC)beta and one cation channel, TRPM5. However, in insects such as the fruit fly, Drosophila melanogaster, it is unclear whether different tastants, such as bitter compounds, are sensed in gustatory receptor neurons (GRNs) through one or multiple ion channels, as the cation channels required in insect GRNs are unknown. Here, we set out to explore additional sensory roles for the Drosophila TRPA1 channel, which was known to function in thermosensation. We found that TRPA1 was expressed in GRNs that respond to aversive compounds. Elimination of TRPA1 had no impact on the responses to nearly all bitter compounds tested, including caffeine, quinine, and strychnine. Rather, we found that TRPA1 was required in a subset of avoidance GRNs for the behavioral and electrophysiological responses to aristolochic acid. TRPA1 did not appear to be activated or inhibited directly by aristolochic acid. We found that elimination of the same PLC that leads to activation of TRPA1 in thermosensory neurons was also required in the TRPA1-expressing GRNs for avoiding aristolochic acid. Given that mammalian TRPA1 is required for responding to noxious chemicals, many of which cause pain and injury, our analysis underscores the evolutionarily conserved role for TRPA1 channels in chemical avoidance.
Al-Anzi,
Response of Drosophila to wasabi is mediated by painless, the fly homolog of mammalian TRPA1/ANKTM1.
2006, Pubmed
Al-Anzi,
Response of Drosophila to wasabi is mediated by painless, the fly homolog of mammalian TRPA1/ANKTM1.
2006,
Pubmed
Bandell,
Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin.
2004,
Pubmed
Bautista,
Pungent products from garlic activate the sensory ion channel TRPA1.
2005,
Pubmed
Bautista,
TRPA1 mediates the inflammatory actions of environmental irritants and proalgesic agents.
2006,
Pubmed
Caterina,
Impaired nociception and pain sensation in mice lacking the capsaicin receptor.
2000,
Pubmed
Dahanukar,
Two Gr genes underlie sugar reception in Drosophila.
2007,
Pubmed
Dahanukar,
A Gr receptor is required for response to the sugar trehalose in taste neurons of Drosophila.
2001,
Pubmed
Davis,
Vanilloid receptor-1 is essential for inflammatory thermal hyperalgesia.
2000,
Pubmed
Dunipace,
Spatially restricted expression of candidate taste receptors in the Drosophila gustatory system.
2001,
Pubmed
Glendinning,
Electrophysiological evidence for two transduction pathways within a bitter-sensitive taste receptor.
1997,
Pubmed
Glendinning,
Contribution of different taste cells and signaling pathways to the discrimination of "bitter" taste stimuli by an insect.
2002,
Pubmed
Gong,
Ends-out, or replacement, gene targeting in Drosophila.
2003,
Pubmed
Hamada,
An internal thermal sensor controlling temperature preference in Drosophila.
2008,
Pubmed
,
Xenbase
Hiroi,
Differentiated response to sugars among labellar chemosensilla in Drosophila.
2002,
Pubmed
Jiao,
A Drosophila gustatory receptor required for the responses to sucrose, glucose, and maltose identified by mRNA tagging.
2007,
Pubmed
Jiao,
Gr64f is required in combination with other gustatory receptors for sugar detection in Drosophila.
2008,
Pubmed
Jordt,
Mustard oils and cannabinoids excite sensory nerve fibres through the TRP channel ANKTM1.
2004,
Pubmed
Karashima,
TRPA1 acts as a cold sensor in vitro and in vivo.
2009,
Pubmed
Kim,
A TRPV family ion channel required for hearing in Drosophila.
2003,
Pubmed
Kindt,
Caenorhabditis elegans TRPA-1 functions in mechanosensation.
2007,
Pubmed
Kwan,
TRPA1 contributes to cold, mechanical, and chemical nociception but is not essential for hair-cell transduction.
2006,
Pubmed
Kwon,
Control of thermotactic behavior via coupling of a TRP channel to a phospholipase C signaling cascade.
2008,
Pubmed
Lee,
Multiple gustatory receptors required for the caffeine response in Drosophila.
2009,
Pubmed
Macpherson,
The pungency of garlic: activation of TRPA1 and TRPV1 in response to allicin.
2005,
Pubmed
Meunier,
Peripheral coding of bitter taste in Drosophila.
2003,
Pubmed
Montell,
Drosophila TRP channels.
2005,
Pubmed
Montell,
A taste of the Drosophila gustatory receptors.
2009,
Pubmed
Moon,
A taste receptor required for the caffeine response in vivo.
2006,
Pubmed
Moon,
A Drosophila gustatory receptor essential for aversive taste and inhibiting male-to-male courtship.
2009,
Pubmed
Niemeyer,
The Drosophila light-activated conductance is composed of the two channels TRP and TRPL.
1996,
Pubmed
Obata,
TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury.
2005,
Pubmed
Pearn,
Molecular, biochemical, and electrophysiological characterization of Drosophila norpA mutants.
1996,
Pubmed
Prober,
Zebrafish TRPA1 channels are required for chemosensation but not for thermosensation or mechanosensory hair cell function.
2008,
Pubmed
,
Xenbase
Pérez,
A transient receptor potential channel expressed in taste receptor cells.
2002,
Pubmed
,
Xenbase
Rosenzweig,
The Drosophila ortholog of vertebrate TRPA1 regulates thermotaxis.
2005,
Pubmed
Slone,
Sugar receptors in Drosophila.
2007,
Pubmed
Story,
ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures.
2003,
Pubmed
Talavera,
Heat activation of TRPM5 underlies thermal sensitivity of sweet taste.
2005,
Pubmed
Talavera,
Nicotine activates the chemosensory cation channel TRPA1.
2009,
Pubmed
Thorne,
Taste perception and coding in Drosophila.
2004,
Pubmed
Tracey,
painless, a Drosophila gene essential for nociception.
2003,
Pubmed
Ueno,
Trehalose sensitivity in Drosophila correlates with mutations in and expression of the gustatory receptor gene Gr5a.
2001,
Pubmed
Venkatachalam,
TRP channels.
2007,
Pubmed
Viswanath,
Opposite thermosensor in fruitfly and mouse.
2003,
Pubmed
Vosshall,
Molecular architecture of smell and taste in Drosophila.
2007,
Pubmed
Wang,
Taste representations in the Drosophila brain.
2004,
Pubmed
Wang,
Dissecting independent channel and scaffolding roles of the Drosophila transient receptor potential channel.
2005,
Pubmed
Yoon,
Specific molecular alterations in the norpA-encoded phospholipase C of Drosophila and their effects on electrophysiological responses in vivo.
2004,
Pubmed
Zhang,
Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways.
2003,
Pubmed
van der Goes van Naters,
Insects as chemosensors of humans and crops.
2006,
Pubmed
