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Dev Growth Differ October 1, 2003; 45 (5-6): 463-71.

In vitro induction and transplantation of eye during early Xenopus development.

Sedohara A , Komazaki S , Asashima M .

A vertebrate eye was induced via a series of coordinated inductive interactions. Here, we describe a novel in vitro system to induce eye formation at high frequency using Xenopus early gastrulae. The eye formed in vitro is morphologically similar to the normal eye. When the in vitro eye was transplanted into a stage-33 tadpole, the optic nerve was seen extending from the grafted eye to the tectum of the host brain and the in vitro eye graft was retained after metamorphosis. In addition, we transplanted the eye formed in vitro into a tadpole with both eyes removed. The resultant juvenile frogs could perceive brightness using the grafted eye and thereby control their skin color, suggesting that the eye formed in vitro could function normally.

PubMed ID: 14706071
Article link: Dev Growth Differ

Species referenced: Xenopus
Genes referenced: hoxb9 ncam1 odc1 otx2 rpe

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