Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-41667
Mol Cell Neurosci January 1, 2010; 43 (1): 117-26.

Molecular mechanisms underlying membrane-potential-mediated regulation of neuronal K2P2.1 channels.

Segal-Hayoun Y , Cohen A , Zilberberg N .


Abstract
The activity of background K(2P) channels adjusts the resting membrane potential to enable plasticity of excitable cells. Here we have studied the regulation of neuronal K(2P)2.1 (KCNK2, TREK-1) channel activity by resting membrane potential. When heterologously expressed, K(2P)2.1 currents gradually increased at hyperpolarizing potentials and declined at depolarizing potentials, with a midpoint potential of -60 mV. As K(2P) channels are not equipped with an integral voltage sensor, we sought extrinsic cellular components that could convert changes in the membrane electrical field to cellular activity that would indirectly modify K(2P)2.1 currents. We propose that membrane depolarization activated the Gq protein-coupled receptor pathway, in the apparent absence of ligand, resulting in phosphatidylinositol-4,5-bisphosphate (PIP(2)) depletion through the action of phospholipase C. Our results suggest a novel mechanism in which an indirect pathway confers membrane potential regulation onto channels that are not intrinsically voltage sensitive to enhance regulation of neuronal excitability levels.

PubMed ID: 19837167
Article link: Mol Cell Neurosci

Genes referenced: kcnk2



Xenbase: The Xenopus laevis and X. tropicalis resource.
Version: 4.11.2


Major funding for Xenbase is provided by grant P41 HD064556