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XB-ART-41701
Biochem Biophys Res Commun June 18, 2010; 397 (1): 75-81.

Xclaudin 1 is required for the proper gastrulation in Xenopus laevis.

Chang DJ , Hwang YS , Cha SW , Chae JP , Hwang SH , Hahn JH , Bae YC , Lee HS , Park MJ .


Abstract
Claudin 1 is one of the tight junctional proteins involved in the tight sealing of the cellular sheets and plays a crucial role in the maintenance of cell polarity. Although its structure and physiological function in intercellular adhesion is relatively well understood, we have little information about its possible involvement in early development of vertebrates. We found Xclaudin 1 is expressed maternally in the oocyte of Xenopus laevis and the zygotic expression initiates stage 9 in the animal hemisphere but not in the vegetal hemisphere, limited on the ectoderm and mesoderm until the end of gastrulation. We have investigated a potential role for claudin 1 at gastrulation by gain and loss-of-function studies. Over-expression of Xclaudin 1 resulted in gastrulation defect in a dose-dependent manner. Knockdown of Xclaudin 1 by antisense morpholino oligonucleotides (MOs) blocked convergent extension, whereas ectopic expression of Xclaudin 1-myc mRNA rescued these defects. However, altered expression of Xclaudin 1 did not inhibit mesodermal gene expression. Taken together, our results suggest that Xclaudin 1 is required for proper convergent extension movement during Xenopus gastrulation.

PubMed ID: 20576541
Article link: Biochem Biophys Res Commun

Genes referenced: bmp4 chrd.1 cldn1 gsc myc odc1 tbxt wnt11
Morpholinos: cldn1 MO1


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