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Dev Cell
2010 Jul 20;191:138-47. doi: 10.1016/j.devcel.2010.06.008.
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Epidermal wound repair is regulated by the planar cell polarity signaling pathway.
Caddy J
,
Wilanowski T
,
Darido C
,
Dworkin S
,
Ting SB
,
Zhao Q
,
Rank G
,
Auden A
,
Srivastava S
,
Papenfuss TA
,
Murdoch JN
,
Humbert PO
,
Parekh V
,
Boulos N
,
Weber T
,
Zuo J
,
Cunningham JM
,
Jane SM
.
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The mammalian PCP pathway regulates diverse developmental processes requiring coordinated cellular movement, including neural tube closure and cochlear stereociliary orientation. Here, we show that epidermal wound repair is regulated by PCP signaling. Mice carrying mutant alleles of PCP genes Vangl2, Celsr1, PTK7, and Scrb1, and the transcription factor Grhl3, interact genetically, exhibiting failed wound healing, neural tube defects, and disordered cochlear polarity. Using phylogenetic analysis, ChIP, and gene expression in Grhl3(-)(/-) mice, we identified RhoGEF19, a homolog of a RhoA activator involved in PCP signaling in Xenopus, as a direct target of GRHL3. Knockdown of Grhl3 or RhoGEF19 in keratinocytes induced defects in actin polymerization, cellular polarity, and wound healing, and re-expression of RhoGEF19 rescued these defects in Grhl3-kd cells. These results define a role for Grhl3 in PCP signaling and broadly implicate this pathway in epidermal repair.
Adler,
Planar signaling and morphogenesis in Drosophila.
2002, Pubmed
Adler,
Planar signaling and morphogenesis in Drosophila.
2002,
Pubmed
Axelrod,
Coupling planar cell polarity signaling to morphogenesis.
2002,
Pubmed
Blair,
Twinstar, the Drosophila homolog of cofilin/ADF, is required for planar cell polarity patterning.
2006,
Pubmed
Brock,
Healing of incisional wounds in the embryonic chick wing bud: characterization of the actin purse-string and demonstration of a requirement for Rho activation.
1996,
Pubmed
Curtin,
Mutation of Celsr1 disrupts planar polarity of inner ear hair cells and causes severe neural tube defects in the mouse.
2003,
Pubmed
Dabdoub,
Wnt signaling mediates reorientation of outer hair cell stereociliary bundles in the mammalian cochlea.
2003,
Pubmed
DesMarais,
Cofilin takes the lead.
2005,
Pubmed
Devenport,
Planar polarization in embryonic epidermis orchestrates global asymmetric morphogenesis of hair follicles.
2008,
Pubmed
Dow,
The tumour-suppressor Scribble dictates cell polarity during directed epithelial migration: regulation of Rho GTPase recruitment to the leading edge.
2007,
Pubmed
Durmus,
Expression analysis of the novel gene collagen triple helix repeat containing-1 (Cthrc1).
2006,
Pubmed
Fanto,
Nuclear signaling by Rac and Rho GTPases is required in the establishment of epithelial planar polarity in the Drosophila eye.
2000,
Pubmed
Fukata,
Roles of Rho-family GTPases in cell polarisation and directional migration.
2003,
Pubmed
Gurniak,
The actin depolymerizing factor n-cofilin is essential for neural tube morphogenesis and neural crest cell migration.
2005,
Pubmed
Habas,
Wnt/Frizzled activation of Rho regulates vertebrate gastrulation and requires a novel Formin homology protein Daam1.
2001,
Pubmed
,
Xenbase
Hall,
Rho GTPases and the control of cell behaviour.
2005,
Pubmed
Hamblet,
Dishevelled 2 is essential for cardiac outflow tract development, somite segmentation and neural tube closure.
2002,
Pubmed
Hislop,
Grhl3 and Lmo4 play coordinate roles in epidermal migration.
2008,
Pubmed
Kalabis,
Vangl1 protein acts as a downstream effector of intestinal trefoil factor (ITF)/TFF3 signaling and regulates wound healing of intestinal epithelium.
2006,
Pubmed
Keller,
Shaping the vertebrate body plan by polarized embryonic cell movements.
2002,
Pubmed
Kibar,
Ltap, a mammalian homolog of Drosophila Strabismus/Van Gogh, is altered in the mouse neural tube mutant Loop-tail.
2001,
Pubmed
Kim,
Essential role for beta-arrestin 2 in the regulation of Xenopus convergent extension movements.
2007,
Pubmed
,
Xenbase
Klein,
Planar cell polarization: an emerging model points in the right direction.
2005,
Pubmed
Kodama,
Coordinating cytoskeletal tracks to polarize cellular movements.
2004,
Pubmed
Lee,
The grainy head transcription factor is essential for the function of the frizzled pathway in the Drosophila wing.
2004,
Pubmed
Lu,
PTK7/CCK-4 is a novel regulator of planar cell polarity in vertebrates.
2004,
Pubmed
,
Xenbase
Mace,
An epidermal barrier wound repair pathway in Drosophila is mediated by grainy head.
2005,
Pubmed
Martin,
Wound healing--aiming for perfect skin regeneration.
1997,
Pubmed
Martin,
Parallels between tissue repair and embryo morphogenesis.
2004,
Pubmed
McCluskey,
Analysis of the tissue movements of embryonic wound healing--DiI studies in the limb bud stage mouse embryo.
1995,
Pubmed
Montcouquiol,
Identification of Vangl2 and Scrb1 as planar polarity genes in mammals.
2003,
Pubmed
Murdoch,
Circletail, a new mouse mutant with severe neural tube defects: chromosomal localization and interaction with the loop-tail mutation.
2001,
Pubmed
Murdoch,
Disruption of scribble (Scrb1) causes severe neural tube defects in the circletail mouse.
2003,
Pubmed
Park,
Role of PKA as a negative regulator of PCP signaling pathway during Xenopus gastrulation movements.
2006,
Pubmed
,
Xenbase
Pyagay,
Collagen triple helix repeat containing 1, a novel secreted protein in injured and diseased arteries, inhibits collagen expression and promotes cell migration.
2005,
Pubmed
Redd,
Wound healing and inflammation: embryos reveal the way to perfect repair.
2004,
Pubmed
Ren,
Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton.
1999,
Pubmed
Seifert,
Frizzled/PCP signalling: a conserved mechanism regulating cell polarity and directed motility.
2007,
Pubmed
Shimizu,
ROCK-I regulates closure of the eyelids and ventral body wall by inducing assembly of actomyosin bundles.
2005,
Pubmed
Siepel,
Evolutionarily conserved elements in vertebrate, insect, worm, and yeast genomes.
2005,
Pubmed
Strutt,
The role of RhoA in tissue polarity and Frizzled signalling.
1997,
Pubmed
Tanegashima,
WGEF activates Rho in the Wnt-PCP pathway and controls convergent extension in Xenopus gastrulation.
2008,
Pubmed
,
Xenbase
Ting,
A homolog of Drosophila grainy head is essential for epidermal integrity in mice.
2005,
Pubmed
Ting,
The identification and characterization of human Sister-of-Mammalian Grainyhead (SOM) expands the grainyhead-like family of developmental transcription factors.
2003,
Pubmed
Ting,
Inositol- and folate-resistant neural tube defects in mice lacking the epithelial-specific factor Grhl-3.
2003,
Pubmed
Tscharntke,
Impaired epidermal wound healing in vivo upon inhibition or deletion of Rac1.
2007,
Pubmed
Vaezi,
Actin cable dynamics and Rho/Rock orchestrate a polarized cytoskeletal architecture in the early steps of assembling a stratified epithelium.
2002,
Pubmed
Van Aelst,
Role of Rho family GTPases in epithelial morphogenesis.
2002,
Pubmed
Wallingford,
Dishevelled controls cell polarity during Xenopus gastrulation.
2000,
Pubmed
,
Xenbase
Wang,
Regulation of polarized extension and planar cell polarity in the cochlea by the vertebrate PCP pathway.
2005,
Pubmed
Wang,
WGEF is a novel RhoGEF expressed in intestine, liver, heart, and kidney.
2004,
Pubmed
Wilanowski,
Perturbed desmosomal cadherin expression in grainy head-like 1-null mice.
2008,
Pubmed
Wilanowski,
A highly conserved novel family of mammalian developmental transcription factors related to Drosophila grainyhead.
2002,
Pubmed
Winter,
Drosophila Rho-associated kinase (Drok) links Frizzled-mediated planar cell polarity signaling to the actin cytoskeleton.
2001,
Pubmed
Wu,
A quest for the mechanism regulating global planar cell polarity of tissues.
2009,
Pubmed
Yamamoto,
Cthrc1 selectively activates the planar cell polarity pathway of Wnt signaling by stabilizing the Wnt-receptor complex.
2008,
Pubmed
Yan,
Rho1 has multiple functions in Drosophila wing planar polarity.
2009,
Pubmed
Yu,
Grainyhead-like factor Get1/Grhl3 regulates formation of the epidermal leading edge during eyelid closure.
2008,
Pubmed
Zallen,
Planar polarity and tissue morphogenesis.
2007,
Pubmed
Zarbalis,
A focused and efficient genetic screening strategy in the mouse: identification of mutations that disrupt cortical development.
2004,
Pubmed
