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XB-ART-41767
J Biol Chem September 17, 2010; 285 (38): 29525-34.

Histone XH2AX is required for Xenopus anterior neural development: critical role of threonine 16 phosphorylation.

Lee SY , Lau AT , Jeong CH , Shim JH , Kim HG , Kim J , Bode AM , Dong Z .


Abstract
A role for histone H2AX, one of the variants of the nucleosome core histone H2A, has been demonstrated in DNA repair, tumor suppression, apoptosis, and cell cycle checkpoint function. However, the physiological function and post-translational modification of histone H2AX during vertebrate development have not been elucidated. Here, we provide evidence showing that Xenopus histone H2AX (XH2AX) has a role in the anterior neural plate for eye field formation during Xenopus embryogenesis. A loss-of-function study clearly demonstrated a critical role of XH2AX in anterior neural specification. Through a differentiation assay with Xenopus animal cap embryonic stem cells, we confirmed that XH2AX is required for the activin-induced anterior neural specification of the ectoderm. Furthermore, we found that Chk1 is an essential kinase to phosphorylate histone XH2AX at Thr(16), which is involved in the biological function of this histone. Taken together, our findings reveal that XH2AX has a specific role in anterior neural formation of Xenopus, which is mediated through phosphorylation of XH2AX at Thr(16) by Chk1.

PubMed ID: 20639511
PMC ID: PMC2937984
Article link: J Biol Chem
Grant support: [+]
Genes referenced: actl6a chek1 chek2 h2ac21 h2ax h2axl h4c4 hoxb9 myc ncam1 otx2 pax6 rax sox3 tubb2b
Morpholinos: chek1 MO1 hist2h2ab MO1


Article Images: [+] show captions
References [+] :
Ariizumi, Isolation and differentiation of Xenopus animal cap cells. 2009, Pubmed, Xenbase


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