Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
2004 Jan 01;242:886-98. doi: 10.1128/MCB.24.2.886-898.2004.
Show Gene links
Show Anatomy links
Drosophila doubletime mutations which either shorten or lengthen the period of circadian rhythms decrease the protein kinase activity of casein kinase I.
Preuss F
,
Fan JY
,
Kalive M
,
Bao S
,
Schuenemann E
,
Bjes ES
,
Price JL
.
???displayArticle.abstract???
In both mammals and fruit flies, casein kinase I has been shown to regulate the circadian phosphorylation of the period protein (PER). This phosphorylation regulates the timing of PER's nuclear accumulation and decline, and it is necessary for the generation of circadian rhythms. In Drosophila melanogaster, mutations affecting a casein kinase I (CKI) ortholog called doubletime (dbt) can produce short or long periods. The effects of both a short-period (dbt(S)) and long-period (dbt(L)) mutation on DBT expression and biochemistry were analyzed. Immunoblot analysis of DBT in fly heads showed that both the dbt(S) and dbt(L) mutants express DBT at constant levels throughout the day. Glutathione S-transferase pull-down assays and coimmunoprecipitation of DBT and PER showed that wild-type DBT, DBT(S), and DBT(L) proteins can bind to PER equivalently and that these interactions are mediated by the evolutionarily conserved N-terminal part of DBT. However, both the dbt(S) and dbt(L) mutations reduced the CKI-7-sensitive kinase activity of an orthologous Xenopus laevis CKIdelta expressed in Escherichia coli. Moreover, expression of DBT in Drosophila S2 cells produced a CKI-7-sensitive kinase activity which was reduced by both the dbt(S) and dbt(L) mutations. Thus, lowered enzyme activity is associated with both short-period and long-period phenotypes.
Akashi,
Control of intracellular dynamics of mammalian period proteins by casein kinase I epsilon (CKIepsilon) and CKIdelta in cultured cells.
2002, Pubmed
Akashi,
Control of intracellular dynamics of mammalian period proteins by casein kinase I epsilon (CKIepsilon) and CKIdelta in cultured cells.
2002,
Pubmed
Akten,
A role for CK2 in the Drosophila circadian oscillator.
2003,
Pubmed
Allada,
A mutant Drosophila homolog of mammalian Clock disrupts circadian rhythms and transcription of period and timeless.
1998,
Pubmed
Allada,
Stopping time: the genetics of fly and mouse circadian clocks.
2001,
Pubmed
Bae,
Circadian regulation of a Drosophila homolog of the mammalian Clock gene: PER and TIM function as positive regulators.
1998,
Pubmed
Bao,
The Drosophila double-timeS mutation delays the nuclear accumulation of period protein and affects the feedback regulation of period mRNA.
2001,
Pubmed
Blau,
Cycling vrille expression is required for a functional Drosophila clock.
1999,
Pubmed
Camacho,
Human casein kinase Idelta phosphorylation of human circadian clock proteins period 1 and 2.
2001,
Pubmed
Cegielska,
Autoinhibition of casein kinase I epsilon (CKI epsilon) is relieved by protein phosphatases and limited proteolysis.
1998,
Pubmed
Ceriani,
Light-dependent sequestration of TIMELESS by CRYPTOCHROME.
1999,
Pubmed
Chang,
A novel C-terminal domain of drosophila PERIOD inhibits dCLOCK:CYCLE-mediated transcription.
2003,
Pubmed
Chijiwa,
A newly synthesized selective casein kinase I inhibitor, N-(2-aminoethyl)-5-chloroisoquinoline-8-sulfonamide, and affinity purification of casein kinase I from bovine testis.
1989,
Pubmed
Claridge-Chang,
Circadian regulation of gene expression systems in the Drosophila head.
2001,
Pubmed
Curtin,
Temporally regulated nuclear entry of the Drosophila period protein contributes to the circadian clock.
1995,
Pubmed
Cyran,
vrille, Pdp1, and dClock form a second feedback loop in the Drosophila circadian clock.
2003,
Pubmed
Darlington,
The period E-box is sufficient to drive circadian oscillation of transcription in vivo.
2000,
Pubmed
Darlington,
Closing the circadian loop: CLOCK-induced transcription of its own inhibitors per and tim.
1998,
Pubmed
Edery,
Temporal phosphorylation of the Drosophila period protein.
1994,
Pubmed
Egan,
An extraretinally expressed insect cryptochrome with similarity to the blue light photoreceptors of mammals and plants.
1999,
Pubmed
Eide,
The circadian regulatory proteins BMAL1 and cryptochromes are substrates of casein kinase Iepsilon.
2002,
Pubmed
Emery,
CRY, a Drosophila clock and light-regulated cryptochrome, is a major contributor to circadian rhythm resetting and photosensitivity.
1998,
Pubmed
Gekakis,
Isolation of timeless by PER protein interaction: defective interaction between timeless protein and long-period mutant PERL.
1995,
Pubmed
Glossop,
Interlocked feedback loops within the Drosophila circadian oscillator.
1999,
Pubmed
Glossop,
VRILLE feeds back to control circadian transcription of Clock in the Drosophila circadian oscillator.
2003,
Pubmed
Graves,
Role of COOH-terminal phosphorylation in the regulation of casein kinase I delta.
1995,
Pubmed
Hao,
A circadian enhancer mediates PER-dependent mRNA cycling in Drosophila melanogaster.
1997,
Pubmed
Hardin,
Feedback of the Drosophila period gene product on circadian cycling of its messenger RNA levels.
1990,
Pubmed
Hayasaka,
In vivo disruption of Xenopus CLOCK in the retinal photoreceptor cells abolishes circadian melatonin rhythmicity without affecting its production levels.
2002,
Pubmed
,
Xenbase
Hunter-Ensor,
Regulation of the Drosophila protein timeless suggests a mechanism for resetting the circadian clock by light.
1996,
Pubmed
Keesler,
Phosphorylation and destabilization of human period I clock protein by human casein kinase I epsilon.
2000,
Pubmed
Kloss,
The Drosophila clock gene double-time encodes a protein closely related to human casein kinase Iepsilon.
1998,
Pubmed
Kloss,
Phosphorylation of period is influenced by cycling physical associations of double-time, period, and timeless in the Drosophila clock.
2001,
Pubmed
Ko,
Role for Slimb in the degradation of Drosophila Period protein phosphorylated by Doubletime.
2002,
Pubmed
Konopka,
Clock mutants of Drosophila melanogaster.
1971,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lee,
PER and TIM inhibit the DNA binding activity of a Drosophila CLOCK-CYC/dBMAL1 heterodimer without disrupting formation of the heterodimer: a basis for circadian transcription.
1999,
Pubmed
Lee,
Posttranslational mechanisms regulate the mammalian circadian clock.
2001,
Pubmed
Leloup,
A model for circadian rhythms in Drosophila incorporating the formation of a complex between the PER and TIM proteins.
1998,
Pubmed
Lin,
Photic signaling by cryptochrome in the Drosophila circadian system.
2001,
Pubmed
Lin,
Influence of the period-dependent circadian clock on diurnal, circadian, and aperiodic gene expression in Drosophila melanogaster.
2002,
Pubmed
Lin,
A role for casein kinase 2alpha in the Drosophila circadian clock.
,
Pubmed
Lowrey,
Positional syntenic cloning and functional characterization of the mammalian circadian mutation tau.
2000,
Pubmed
Lyons,
Specific sequences outside the E-box are required for proper per expression and behavioral rescue.
2000,
Pubmed
Martinek,
A role for the segment polarity gene shaggy/GSK-3 in the Drosophila circadian clock.
2001,
Pubmed
McDonald,
Microarray analysis and organization of circadian gene expression in Drosophila.
2001,
Pubmed
McNeil,
A molecular rhythm mediating circadian clock output in Drosophila.
1998,
Pubmed
Myers,
Light-induced degradation of TIMELESS and entrainment of the Drosophila circadian clock.
1996,
Pubmed
Price,
double-time is a novel Drosophila clock gene that regulates PERIOD protein accumulation.
1998,
Pubmed
Price,
Suppression of PERIOD protein abundance and circadian cycling by the Drosophila clock mutation timeless.
1995,
Pubmed
Renn,
A pdf neuropeptide gene mutation and ablation of PDF neurons each cause severe abnormalities of behavioral circadian rhythms in Drosophila.
1999,
Pubmed
Rothenfluh,
Short-period mutations of per affect a double-time-dependent step in the Drosophila circadian clock.
2000,
Pubmed
Rouyer,
A new gene encoding a putative transcription factor regulated by the Drosophila circadian clock.
1997,
Pubmed
Rutila,
CYCLE is a second bHLH-PAS clock protein essential for circadian rhythmicity and transcription of Drosophila period and timeless.
1998,
Pubmed
Rutter,
Regulation of clock and NPAS2 DNA binding by the redox state of NAD cofactors.
2001,
Pubmed
Saez,
Regulation of nuclear entry of the Drosophila clock proteins period and timeless.
1996,
Pubmed
Sarov-Blat,
The Drosophila takeout gene is a novel molecular link between circadian rhythms and feeding behavior.
2000,
Pubmed
Schotland,
Altered entrainment and feedback loop function effected by a mutant period protein.
2000,
Pubmed
Sehgal,
Loss of circadian behavioral rhythms and per RNA oscillations in the Drosophila mutant timeless.
1994,
Pubmed
Sehgal,
Rhythmic expression of timeless: a basis for promoting circadian cycles in period gene autoregulation.
1995,
Pubmed
Shafer,
Sequential nuclear accumulation of the clock proteins period and timeless in the pacemaker neurons of Drosophila melanogaster.
2002,
Pubmed
Sidote,
Differential effects of light and heat on the Drosophila circadian clock proteins PER and TIM.
1998,
Pubmed
Stanewsky,
The cryb mutation identifies cryptochrome as a circadian photoreceptor in Drosophila.
1998,
Pubmed
Stanewsky,
Multiple circadian-regulated elements contribute to cycling period gene expression in Drosophila.
1997,
Pubmed
Suri,
Two novel doubletime mutants alter circadian properties and eliminate the delay between RNA and protein in Drosophila.
2000,
Pubmed
Takano,
Cloning and characterization of rat casein kinase 1epsilon.
2000,
Pubmed
Toh,
An hPer2 phosphorylation site mutation in familial advanced sleep phase syndrome.
2001,
Pubmed
Ueda,
Genome-wide transcriptional orchestration of circadian rhythms in Drosophila.
2002,
Pubmed
Vielhaber,
Nuclear entry of the circadian regulator mPER1 is controlled by mammalian casein kinase I epsilon.
2000,
Pubmed
Vosshall,
Block in nuclear localization of period protein by a second clock mutation, timeless.
1994,
Pubmed
Wang,
Regulation of the cycling of timeless (tim) RNA.
2001,
Pubmed
Williams,
Molecular components of the circadian system in Drosophila.
2001,
Pubmed
Williams,
A circadian output in Drosophila mediated by neurofibromatosis-1 and Ras/MAPK.
2001,
Pubmed
Young,
Time zones: a comparative genetics of circadian clocks.
2001,
Pubmed
Zeng,
A light-entrainment mechanism for the Drosophila circadian clock.
1996,
Pubmed
Zeng,
Constitutive overexpression of the Drosophila period protein inhibits period mRNA cycling.
1994,
Pubmed
Zerr,
Circadian fluctuations of period protein immunoreactivity in the CNS and the visual system of Drosophila.
1990,
Pubmed