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XB-ART-41932
Dev Cell August 17, 2010; 19 (2): 220-31.

beta-Catenin primes organizer gene expression by recruiting a histone H3 arginine 8 methyltransferase, Prmt2.



Abstract
An emerging concept in development is that transcriptional poising presets patterns of gene expression in a manner that reflects a cell''s developmental potential. However, it is not known how certain loci are specified in the embryo to establish poised chromatin architecture as the developmental program unfolds. We find that, in the context of transcriptional quiescence prior to the midblastula transition in Xenopus, dorsal specification by the Wnt/beta-catenin pathway is temporally uncoupled from the onset of dorsal target gene expression, and that beta-catenin establishes poised chromatin architecture at target promoters. beta-catenin recruits the arginine methyltransferase Prmt2 to target promoters, thereby establishing asymmetrically dimethylated H3 arginine 8 (R8). Recruitment of Prmt2 to beta-catenin target genes is necessary and sufficient to establish the dorsal developmental program, indicating that Prmt2-mediated histone H3(R8) methylation plays a critical role downstream of beta-catenin in establishing poised chromatin architecture and marking key organizer genes for later expression.

PubMed ID: 20708585
PMC ID: PMC2923644
Article link: Dev Cell
Grant support: [+]
Genes referenced: ctnnb1 eef1a2 h3-3a lef1 myc myl2 nodal3.1 nodal3.2 nodal5 nodal5.2 nodal5.4 nodal6 odc1 prmt2 sia1
Morpholinos: ctnnb1 MO1 prmt2 MO1


Article Images: [+] show captions
References [+] :
Akkers, A hierarchy of H3K4me3 and H3K27me3 acquisition in spatial gene regulation in Xenopus embryos. 2009, Pubmed, Xenbase


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